Bolat, Zeynep Büşraİşlek, ZeynepŞahin, FikrettinÜçışık, Mehmet Hikmet2023-01-082023-01-082023Bolat, Z. B., İşlek, Z., Şahin, F. & Üçışık, M. H. (2023). Delivery of curcumin within emulsome nanoparticles enhances the anti‑cancer activity in androgen‑dependent prostate cancer cell. Molecular Biology Reports, 50(3), pp. 2531-2543. https://doi.org/10.1007/s11033-022-08208-00301-48511573-4978https://doi.org/10.1007/s11033-022-08208-0https://hdl.handle.net/20.500.13055/366Background Curcumin, a dietary polyphenol isolated from turmeric, is a potent phytochemical possessing intrinsic anticancer activities against various cancer types including prostate cancer. However, low water solubility and bioavailability of the compound are major challenges against its medical use. The objective of this study is to evaluate the therapeutic potential of curcumin-loaded emulsome nanoparticular system, i.e. CurcuEmulsomes, for the treatment of androgen dependent LNCaP prostate cancer cell line. Methods and results The antiproliferative efect of both free curcumin and CurcuEmulsome were investigated comparatively on LNCaP and PNT1A cells. Cell viability data indicates that the inhibition in proliferation of LNCaP cells becomes more efective when curcumin is provided with its emulsome formulation rather than its free form. Corresponding to a therapeutic index of 2.25, Half maximal inhibitory (IC50) and cytotoxic (CC50) concentrations of CurcuEmulsomes for LNCaP and PNT1A cells were estimated as 17.1 µM and 38.6 µM, respectively. The fuorescence signal of autofuorescence curcumin was preserved within the CurcuEmulsomes at 72 h after the treatment. Thus, CurcuEmulsomes prolonged biological activity of curcumin. Induced apoptotic cell death and stimulated cell cycle arrest at G2/M phase were attributed to antiproliferative activity of CurcuEmulsomes. Treatment of LNCaP cells with CurcuEmulsomes increased expression of caspase-3 signifcantly by 11.76-fold, whereas decreased cyclin D1, Bcl-2 and AR expression levels signifcantly by of 0.18, 0.06 and 0.46-fold, respectively. Conclusions Presented safety and anticancer activity of CurcuEmulsomes on LNCaP cell line highlights the potential of CurcuEmulsomes to beneft intrinsic anticancer activities of curcumin in androgen dependent prostate cancer therapyeninfo:eu-repo/semantics/closedAccessAndrogen Dependent Prostate CancerCurcuminEmulsomeAnti-CancerPhytochemicalArticle10.1007/s11033-022-08208-050325312543Q4WOS:0009123775000032-s2.0-85145867298PMID: 36607480Q3