Melekoğlu, AbdullahCeviz, Ayşe BegümEronat, Allison PınarÖztürk, TülinPehlevan, FundaYılmaz-Aydoğan, HülyaÖztürk, Oğuz2023-12-212023-12-212024Melekoğlu, A., Ceviz, A. B., Eronat, A. P., Öztürk, T., Pehlevan, F., Yılmaz-Aydoğan, H., & Öztürk, O. (2024). Synergistic and dose-dependent effects of pinostrobin, pinocembrin and pinobanksin on different breast cancer cell lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 30(1), pp. 23-30. https://doi.org/10.9775/kvfd.2023.301871309-2251https://doi.org/10.9775/kvfd.2023.30187https://hdl.handle.net/20.500.13055/603In this study, the cytotoxic and apoptotic effects of three phenolic compounds highly found in the poplar type propolis; pinostrobin (PS), pinocembrin (PC) and pinobanksin (PB), were investigated individually and in combination on hormon-positive (MCF-7) and triple negative (MDA-MB-231) breast cancer cell lines and fibrocystic breast epithelium (MCF-10A) as control. Assessment of cytotoxicity and apoptosis were performed with WST-1 and Annexin V-7AAD assays, respectively. All statistical analyses including the two-way ANOVA and multiple-t-test were performed using GraphPad Prism software. Individually, PB (P<0.0001), PS (P<0.0001), and PC (P<0.05) demonstrated potent cytotoxic effects at moderate to high doses and late time intervals on MCF-7. PB and PS have been found to have a significant proliferative effect at low doses (P<0.0001), however, this effect disappeared in higher doses in this cell line. Dual combinations of PB+PC and PB+PS were toxic on MCF-10A, however, dual combination of PS+PC and the triple combination (PB+PS+PC) showed no cytotoxicity until high doses at late time intervals (P>0.05). On MCF-7, the triple combination induced cytotoxic/apoptotic effects even with the 25% dose and 50% dose on MDA-MB-231(p<0.0001). Our findings clearly showed that different combinations of these phenolic substances can have synergistic cytotoxic effects and even hormetic effects in non-tumorogenic cells.eninfo:eu-repo/semantics/openAccessBreast Cancer Cell LineCytotoxicityPinobanksinPinocembrinPinostrobinPropolisArticle10.9775/kvfd.2023.301873012330Q3WOS:0011273103000012-s2.0-85179667174Q3