Erol Bozkurt, AyşeSüleymanoğlu, MedihaCinek, TuğçeKarali, NilgünBallıkaya, Sedatİyikesici, Mehmet SalihKuruca, Serap2026-08-012026-08-012026Erol Bozkurt, A., Süleymanoğlu, M., Cinek, T., Karali, N., Ballıkaya, S., İyikesici, M. S., & Kuruca, S. (2026). The effect of radiofrequency exposure on the cytotoxic activity of sunitinib and a novel sunitinib-class compound 4, in colorectal cancer cells. International Journal of Molecular Sciences, 27(13), pp. 1-16. https://doi.org/10.3390/ijms271359531422-0067https://doi.org/10.3390/ijms27135953https://hdl.handle.net/20.500.13055/1574Achieving optimal therapeutic efficacy in colorectal cancer (CRC) remains challenging, highlighting the need for novel treatment strategies. This study evaluated whether radiofre quency (RF) hyperthermia enhances the anticancer effects of sunitinib malate and a newly synthesized tyrosine kinase (TK) inhibitor (compound 4). HCT116 CRC cells and BEAS-2B normal epithelial cells were treated with increasing concentrations of both agents in the presence or absence of RF exposure using a mobile RF device. Cell viability and death were assessed by MTT assay and flow cytometry (Annexin V/PI). RF exposure was associated with reduced viability and increased cell death in HCT116 cells (p < 0.05). Flow cytometric analysis confirmed that RF exposure may contribute to the observed reduction in viability. Notably, the findings suggest a possible beneficial contribution of RF hyperthermia to the observed cytotoxic effects, particularly near the IC50 concentration. In contrast, BEAS-2B cells maintained high viability with no significant changes (p > 0.05). These findings sug gest that RF exposure may contribute to the observed cytotoxic responses in CRC cells while exerting limited effects on non-malignant cells, suggesting a promising strategy for targeted therapy.eninfo:eu-repo/semantics/openAccessColorectal CancerRadiofrequency HyperthermiaCell ViabilityTyrosine Kinase Inhibitor2-IndolinoneSunitinibArticle10.3390/ijms271359532713116Q1WOS:0018185835000012-s2.0-105045016000PMID: 42450219Q1