Binay, EmrullahÖnal, CemÖnal, ArmağanKepekçi Tekkeli, Şerife Evrim2024-02-282024-02-282024Binay, E., Önal, C., Önal, A., & Kepekçi Tekkeli, Ş. E. (2023). Pharmacokinetic analysis of febuxostat in human serum by UFLC based on fluorimetric derivatization. Revue Roumaine de Chimie, 68(10–12), pp. 527–532. https://doi.org/10.33224/rrch.2023.68.10-12.040035-3930https://doi.org/10.33224/rrch.2023.68.10-12.04https://hdl.handle.net/20.500.13055/658An ultra fast liquid chromatographic (UFLC) method using fluorimetric detection for the quantitation of Febuxostat (FEB) in human serum was developed by precolumn derivatization with 4- bromomethyl-7-methoxy coumarin (BrMmC). The derivatization was catalyzed with dibenzo-18-crown-6-ether to produce a fluorescent derivative which was determined through fluorescence measurement with excitation at 320 nm and emission at 395 nm wavelengths. A C18 column with 100 cm  4.6 mm, 3 µm particule size was used for the UFLC separation procedure. A mixture of acetonitrile: methanol: 0.05 M aqueous ammonium acetate (pH 5.0) (40:40:20, v/v/v) was used as mobile phase. During the procedure, the flow rate was maintained at 0.5 mL/min. A calibration curve was then plotted as 0.005–3.0 µg/mL with a detection limit of 0.0022 µg/mL and quantification limit of 0.0073 µg/mL. The mean recovery to reveal the accuracy and relative standard deviation (RSD) that indicates the precision of the method were found out to be 85.20 % and less than 3.52 %, respectively. The utility of the new method has been demonstrated by measuring the pharmacokinetics of FEB by administration of 80 mg tablets to a healthy female volunteer, aged 42.eninfo:eu-repo/semantics/openAccess4-Bromomethyl-7-Methoxy CoumarinFebuxostatFluorescence DetectionHuman SerumUltra Fast Liquid ChromatographyArticle10.33224/rrch.2023.68.10-12.046810-12527532Q4WOS:0012666218000042-s2.0-85185126291Q4