Temizkan Özdamar, Kevser2026-06-112026-06-112026Temizkan Özdamar, K. (2026). Controlled bio-catalytic synthesis of ampyrone–vanillin macromolecules: Deciphering the transition from nano-oligomers to polymers and the limits of enzymatic stability. Inorganic Chemistry Communications, 190, pp. 1-14. https://doi.org/10.1016/j.inoche.2026.1168591387-70031879-0259https://doi.org/10.1016/j.inoche.2026.116859https://hdl.handle.net/20.500.13055/1511https://doi.org/10.1016/j.inoche.2026.116859This study reports the time-controlled biocatalytic synthesis of nanostructured macromolecules derived from heteroaromatic ampyrone and bio-based vanillin. By optimizing the reaction duration (2, 4, and 6 h) with Horseradish Peroxidase (HRP), stable nano-oligomers (NSO-1, NSO-2) and a robust polymer (nano-pores polymer/NP-P) were successfully isolated. The novelty of this work lies in the precise temporal mapping of the HRP-catalyzed process, identifying a 6 h ‘stability window’ that maximizes molecular growth (Mw = 5743 g/ mol, n ≈ 14) before the onset of enzymatic chain scission. Characterization revealed a significant reduction in the optical band gap from 2.38 eV to 1.49 eV and a morphological transition from macrocrystalline rods to nano porous matrices. These results demonstrate that temporal control in enzymatic synthesis enables fine-tuning of optoelectronic and thermal properties, positioning these sustainable hybrids as viable candidates for organic photovoltaics and smart material technologies.eninfo:eu-repo/semantics/closedAccessBiocatalysisHRP-Mediated PolymerizationReaction KineticsAmpyrone-Vanillin HybridsThermal StabilityNano-ArchitecturesArticle10.1016/j.inoche.2026.116859190114Q1WOS:0017833416000012-s2.0-105039766029Q1