Arıkan, GürkanTuran, VolkanKürek Eken, MeryemGöksoy, Hasan SamiDoğusan, Zeynep2023-01-222023-01-222023Arıkan, G., Turan, V., Kürek Eken, M., Göksoy, H. S. & Doğusan, Z. (2023). Autologous bone marrow-derived nucleated cell (aBMNC) transplantation improves endometrial function in patients with refractory Asherman's syndrome or with thin and dysfunctional endometrium. Journal of Assisted Reproduction and Genetics, 40(5), pp. 1163-1171. https://doi.org/10.1007/s10815-023-02727-w1058-0468https://doi.org/10.1007/s10815-023-02727-whttps://hdl.handle.net/20.500.13055/377Purpose: The purpose was to evaluate the effect of intrauterine injection of aBMNC on the endometrial function in patients with refractory Asherman's syndrome (AS) and/or thin and dysfunctional endometrium (TE). Study design: This is a prospective, experimental, non-controlled study MATERIAL AND METHODS: The study was carried out between December 2018 and December 2020 on 20 patients, who were of age < 45 years and had oligo/amenorrhea and primary infertility due to refractory AS and/or TE. One hundred ml BM was extracted. aBMNC cells were separated according to generic volume reduction protocol by using the Cell Separation System SEPAX S-100 table top centrifuge system. We have evaluated CD34+, mononuclear cell (MNC), and total nucleated cell (TNC) counts. The transplantation aBMNC was performed by two intrauterine injections at an interval of one week, transvaginally into the endometrial-myometrial junction by an ovum aspiration needle. Midcyclic endometrial thickness (ET) and gestations after transplantation were evaluated. Results: The mean TNC, MNC, and CD34+ cells were 11.55 ± 4.7 × 108, 3.85 ± 2.01 × 108, and 7.00 ± 2.88 × 106 at first injection, respectively, and 6.85 ± 2.67 × 108, 2.04 ± 1.11 × 108, and 3.44 ± 1.31 × 106 at second injection, respectively. The maximum posttransplantation ET was significantly higher than the maximum pretransplantation ET: 2.97 ± 0.48 vs. 5.76 ± 1.19 (mean ± standard deviation, p < 0.01). Twelve patients had frozen-thaw embryo transfers after the study. In 42% (n = 5 of 12) of the patients, pregnancy was achieved. One of the five patients delivered a healthy baby at term. Conclusions: Autologous BMNC transplantation may contribute to endometrial function in patients with AS and/or TE.eninfo:eu-repo/semantics/closedAccessAsherman’s SyndromeAutologous Bone MarrowEndometriumStem CellsThin EndometriumTotal Nucleated CellsArticle10.1007/s10815-023-02727-w40511631171Q2WOS:0009179741000012-s2.0-85146584085PMID: 36662355Q1