Özkara, GülçinCeviz, Ayşe BegümEronat, Allison PinarPehlevan Karabıyık, FundaCandan, GoncaÖzturk, OğuzYılmaz Aydoğan, Hülya2025-05-072025-05-072025Özkara, G., Ceviz, A. B., Eronat, A. P., Pehlevan Karabıyık, F., Candan, G., Özturk, O., & Yılmaz Aydoğan, H. (2025). Cytotoxic and anti-migratory effects of polyphenolic compounds on breast cancer cells by altering Jam-A, LFA-1, and VLA-4 gene expression. Natural Product Research, pp. 1-12. https://doi.org/10.1080/14786419.2025.24946291478-64191478-6427https://doi.org/10.1080/14786419.2025.2494629https://hdl.handle.net/20.500.13055/977This study represents the initial research of the effects of a com bination of the largest number (13) of different polyphenic sub stances (PFK5120), formulated based on the propolis content on cell viability, migration and expression of lymphocyte function-associated antigen-1 (LFA-1), very late antigen-4 (VLA-4) and junction adhesion molecule A (Jam-A) in breast cancer (BC) cells. PFK5120 negatively affected cell viability at a 5% concentra tion as compared with unexposed ones (p<0.001). Treatment with 20% PFK5120 for 48h down-regulated Jam-A in MCF-7 and MCF-10A, up-regulated LFA-1 in MCF-10A and MDA-MB-231, and down-regulated VLA-4 in MCF-10A and MDA-MB-231 (p<0.001). Furthermore, migration was found to be inhibited by PFK5120 at varying doses and times. Migration was completely inhibited by 35% PFK5120 treatment in MDA-MB-231, while even lower concen trations (10%) were effective in MCF-7. Current findings indicate that PFK5120 represents a valuable natural component of BC ther apy through its cytotoxic and anti-migratory effects.eninfo:eu-repo/semantics/openAccessPolyphenolsBreast CancerCell MigrationJam-ALFA-1VLA-4Article10.1080/14786419.2025.2494629112Q3Q1