Şerefoğlu, Ege CanKolbaşı, BircanBülbül, Muhammet VolkanKarabulut, SedaÇakıcı, ÇağrıGündoğdu Özdemir, Reyhan ZeynepKeskin, İlknur2024-10-092024-10-092024Şerefoğlu, E. C., Kolbaşı, B., Bülbül, M. V., Karabulut, S., Çakıcı, Ç., Gündoğdu Özdemir, R. Z., & Keskin, İ. (2024). Therapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model. The World Journal of Men's Health. https://doi.org/10.5534/wjmh.2400592287-42082287-4690https://doi.org/10.5534/wjmh.240059https://hdl.handle.net/20.500.13055/818Purpose: This study aimed to examine the therapeutic effects of injection of conditioned medium of adipose-derived mesen chymal stem cells (ADMSC-CM) in a surgically created varicocele model in comparison with varicocelectomy. Materials and Methods: Twenty-eight male Wistar Albino rats were randomly divided into four groups: sham group, varico cele group, varicocelectomy group, and ADMSC-CM injection group. Sperm parameters were analyzed in samples taken from the epididymis after treatment. Malondialdehyde and superoxide dismutase (SOD) levels in blood samples were exam ined by biochemical analysis. The testicular tissues were stained with hematoxylin-eosin for histological examination (John sen’s Score). Additionally, Western Blot analyzes were performed to detect Claudin-11 levels, the functional protein of the blood-testis barrier, in testicular tissues. Results: Varicocelectomy and ADMSC-CM treatments significantly improved mean sperm parameters (concentration, pro gressive motility, motility, normal sperm morphology) (p≤0.05 for all). Both treatment groups had increased SOD levels along with a decrease in malondialdehyde levels (p≤0.05 for all). No significant difference was observed between the ADMSC CM group and the varicocelectomy group in preserving normal testicular histology according to Johnsen’s Score (p=0.114). Levels of Claudin-11 were significantly higher in the varicocelectomy and ADMSC-CM groups compared to the varicocele group (p≤0.05 for all). Conclusions: The therapeutic effects of ADMSC-CM in varicocele model may involve secretion of anti-inflammatory and re generative factors from ADMSC. ADMSC-CM injection appears to be a promising new strategy in the treatment of varicoceleninfo:eu-repo/semantics/openAccessClaudinsCulture MediaConditionedMesenchymal Stem CellsInfertilityVaricoceleArticle10.5534/wjmh.240059Q1WOS: 001321200800001PMID: 39344116Q1