Emet, AbdulsametÖzdemir, ErdiUçkan Çetinkaya, DuyguKılıç, EmineHashemihesar, RaminYürüker, Ali Celalettin SinanTurhan, Egemen2021-12-072021-12-072021Emet, A., Özdemir, E., Çetinkaya, D. U., Kılıç, E., Hashemihesar, R., Yürüker, A. C. S., & Turhan, E. (18 Nov 2021). Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model. Applied Sciences, 11(22), pp. 1-10. https://doi.org/10.3390/app112210900https://doi.org/10.3390/app112210900https://hdl.handle.net/20.500.13055/69The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (n = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (p = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (p = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.eninfo:eu-repo/semantics/openAccessSegmental bone defectPRPOmentumScaffoldMesenchymal stem cellArticle10.3390/app1122109001122110Q2WOS:0007247433000012-s2.0-85119825642Q2