Bozkurt, Rabia NurFalsafi, Seid RezaŞahin, Selin2025-08-262025-08-262025Bozkurt, R. N., Falsafi, S. R., Şahin, S. (2025). Tailoring natural solvents: Deep eutectic approach for recovering bioactives from Prunus Spinosa L. Chemistry & Biodiversity, pp. 1-14. https://doi.org/10.1002/cbdv.2025018581612-18721612-1880https://doi.org/10.1002/cbdv.202501858https://hdl.handle.net/20.500.13055/1087In this study, several choline chloride (ChCl)-based deep eutectic solvent (DES) combinations were prepared at different molarratios (1/1, 1/2, and 2/1) using amine (urea), polyols (ethylene glycol and glycerol), and carboxylic acids (lactic acid, formic acid,propionic acid, and acetic acid). The performances of the DESs in the homogeniser-assisted extraction of Prunus spinosa L.fresh fruit were compared depending on the total phenolic content (TPC), total anthocyanin content (TAC), and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (antioxidant activity [AA]) yields. Based on the findings of Tukey’s test formultiple comparisons, ChCl/formic acid (2/1) provided the best extraction efficiency with statistically significant differences(p < 0.05). Fourier-transform infrared spectroscopy was used to confirm the formation of the proposed DES. Furthermore, theproposed DES also showed better performance compared to conventional aqueous ethanol (60%, v/v) and water. Then, theselected DES (ChCl/formic acid, 2/1) was employed for the optimization study using the Box-Behnken Design of the responsesurface methodology. The optimum process conditions for the maximum yields of TPC (52.34 mg-GAE/g-FF), TAC (2.09 mg-cyn-3-glu/g-FF), and AA (89.21%) were found to be 50% (v/v) water in the DES, 0.833 g of material, and 69.906 s extractiontime.eninfo:eu-repo/semantics/closedAccessCholine Chloride-Based SolventsDesign of Experiments (Doe)Green ExtractionNatural Solvent SystemsTukey’s TestArticle10.1002/cbdv.202501858114Q3PMID: 40839686Q2