Evaluation of the effects of different remineralisation agents on initial enamel lesions by scanning electron microscope and energy-distributed X-ray analysis

Abstract

Background: This study compared the ability of four remineralization agents to restore demineralised enamel lesions in permanent human incisors. Methods: In total, 40 teeth were included in the study. Two samples were obtained from each tooth. The 80 samples were divided into 10 groups (n = 8 per group). The right halves of the teeth were controls solely subjected to demineralisation and stored in artificial saliva for the duration of the experiment. The left halves of the teeth were remineralised using a 5% NaF-containing fluoride varnish (GC MI varnish; GC Corp.), a toothpaste containing casein phosphopeptide and amorphous calcium (GC tooth mousse; GC Corp.), a water-soluble remineralisation paste containing fluoride, hydroxyapatite, xylitol (Remin Pro; Voco), and ROCS remineralising gel containing Ca, magnesium(Mg) and phosphate (P). After 21 days of remineralisation, scanning electron microscopy and energy-dispersive X-ray (EDX) assessments were performed, and the results were analysed using one-way analysis of variance. Results: EDX analysis showed that the ROCS medical remineralising gel had the lowest Ca/P ratio among the groups (P = .04). Compared with the control groups, the Remin Pro and ROCS medical remineralising gel treatments produced significantly higher Ca/P ratios (P = .014 and P = .025). These results were statistically significant, and P-value <.05 was accepted for statistical significance. Conclusions: The group treated with a fluoride-free, xylitol-containing ROCS medical remineralising gel had the lowest Ca/P ratio. Compared with the control groups, Remin Pro and ROCS remineralising gel appeared effective for repairing initial enamel lesions.