Yazar "Aydemir, Zeynep" seçeneğine göre listele
Listeleniyor 1 - 3 / 3
Sayfa Başına Sonuç
Sıralama seçenekleri
Yayın Electrophoretic approaches for investigating plant-derived compound interactions with dna and proteins(Wiley, 2026) Aydemir, Zeynep; Kaya, Beril Sena; Bakırcı, Meysera; Al Faysal, Abdullah; Kalaycıoğlu, Zeynep; Gölcü, AyşegülPlant-derived compounds are increasingly recognized as promising therapeutic agents due to their broad biological activities and structural diversity, making their interactions with DNA and proteins central to understanding mechanisms of action and guiding drug discovery. Electrophoretic techniques probe these interactions by tracking changes in electrophoretic mobility, conformation, complex formation, and biopolymer integrity. This review surveys electrophoretic strategies for characterizing plant-derived compound-DNA and -protein interactions, covering fundamental DNA-binding modes (intercalation, groove binding, electrostatic association, cleavage), representative phytochemicals and extracts, and analytical challenges of complex plant matrices, including chemical heterogeneity, synergistic and antagonistic effects, and active-constituent identification. Particular attention is given to capillary electrophoresis-based approaches (affinity CE, CE, and pressure-assisted frontal analysis) and gel-based methods (agarose gel electrophoresis, native PAGE, SDS-PAGE). The literature shows capillary electrophoresis excels at quantifying binding affinity, stoichiometry, and specificity, whereas gel electrophoresis better visualizes ligand-induced changes in DNA topology and protein behavior. HSA and BSA dominate as model proteins, while advanced methods remain underapplied to chemically complex extracts, gaps pointing toward broader protein targets and natural-product systems. Integrating mass spectrometry, advanced detection, and data-driven approaches could further strengthen the mechanistic and quantitative reach of electrophoretic analysis in this field.Yayın Green MIP-based electrochemical sensing platform for environmental ivermectin analysis(American Chemical Society, 2026) Aydemir, Zeynep; Kaya, Beril Sena; Dorreh, Setareh; Al Faysal, Abdullah; Erdoğan, Taner; Gölcü, AyşegülIvermectin (IVM), a macrocyclic lactone derived from Streptomyces avermitilis, is widely recognized as a “wonder drug” for its broad-spectrum efficacy against internal and external parasites in human and veterinary medicine. Owing to its potent pharmacological activity, precise quantification of IVM is essential for therapeutic monitoring and dose optimization. In this study, we report the design of a novel electrochemical sensor based on molecularly imprinted polymer (MIP) technology, specifically tailored for the selective detection of IVM. The sensor was fabricated via an electropolymerization strategy employing methacrylic acid (MAA) as the functional monomer and aniline as the comonomer in phosphate-buffered saline (PBS, pH 7.0). To the best of our knowledge, this represents the first electropolymerization-based MIP sensor developed for IVM determination. The resulting MAA-IVM@MIP/GCE sensor was thoroughly characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Electrochemical detection was achieved through an indirect redox probe approach with 5.0 mM [Fe(CN)6] 3−/4−, providing a wide linear range (1 × 10−12 −1 × 10−11 M) and remarkably low limits of detection (LOD: 2.91 × 10−13 M) and quantification (LOQ: 9.71 × 10−13 M). The sensor demonstrated high sensitivity, reproducibility, and selectivity, clearly distinguishing IVM from structurally related compounds. It maintained strong analytical performance in pharmaceutical formulations, biological matrices, and environmental samples such as tap water and soil, showing minimal matrix interference. These results confirm the platform’s robustness and applicability. Density functional theory (DFT) calculations were performed to evaluate template−monomer interactions and determine the optimal template:monomer ratio for the MIP-based sensor. The results revealed that the 1:1 complex exhibited the most favorable binding characteristics, consistent with the experimental findings. In addition, the sensor fabrication strategy was designed in accordance with green analytical chemistry principles. The electropolymerization process was performed in aqueous phosphate-buffered saline under mild conditions without the use of excessive cross-linkers or hazardous reagents. The approach minimizes organic solvent consumption, reduces energy requirements, and enables sensor reusability, thereby contributing to a sustainable and environmentally responsible analytical platform. Overall, this cost-effective, scalable, and environmentally conscious electrochemical sensor provides a practical tool for reliable IVM monitoring and has strong potential for clinical diagnostics, pharmacokinetics, and pharmaceutical quality control.Yayın Integrating green chemistry principles into the fabrication of MIP electrochemical sensors for heavy metal monitoring(Taylor & Francis, 2026) Dorreh, Setareh; Kaya, Beril Sena; Aydemir, Zeynep; Al Faysal, Abdullah; Gölcü, AyşegülHeavy metal contamination remains a critical global environmental issue due to the persistence, bioaccumulation, and toxicity of metal ions such as Pb2+, Cd2+, Hg2+, and As³+. Although conventional analytical techniques provide high sensitivity and accuracy, they often rely on energy-intensive instrumentation, hazardous reagents, and generate considerable chemical waste, raising concerns regarding their environmental sustainability. In this context, molecularly imprinted polymer (MIP)-based electrochemical sensors have emerged as promising alternatives, offering high selectivity, operational simplicity, and compatibility with miniaturized and in situ analysis. This review critically examines the integration of Green Analytical Chemistry (GAC) principles into the design and fabrication of MIP-based electrochemical sensors for heavy metal monitoring. Particular attention is given to material selection, polymerization strategies, template removal approaches, and electrode modification techniques, with emphasis on their environmental implications. The applicability of quantitative greenness assessment tools, including the Analytical Eco-Scale, GAPI, AGREE, and AGREEMIP, is discussed in the context of sensor development workflows, highlighting both their strengths and current limitations in addressing fabrication stages, nanomaterial synthesis, and end-of-life considerations. By identifying methodological bottlenecks, particularly solvent-intensive template removal and limited reusability, this review outlines practical directions for advancing more sustainable sensor platforms. Overall, the work provides a critical framework for aligning analytical performance with environmental responsibility in next-generation MIP-based electrochemical sensing systems.
