Yazar "Seyhan, Mehmet Fatih" seçeneğine göre listele

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  • Küçük Resim
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    The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenol
    (Istanbul University, 2024) Çelik, İncilay; Seyhan, Mehmet Fatih; Ceviz, Ayşe Begüm; Aydoğan, Çağatay; Yılmaz Aydoğan, Hülya; Öztürk, Oğuz
    Background and Aims: Standard treatment regimens for fibrocystic breast disease (FBD) do not provide a permanent cure and have undesirable side effects. This study aims to investigate the therapeutic potential of different honey and propolis, as well as some important polyphenols, on breast epithelial cells (MCF-10A). Materials and Methods: The effects of five honey, two propolis extracts and seven polyphenol samples on the cell viability were assessed the WST-1 assay. Content analysis of the propolis samples was performed using high performance liquid chromatography (HPLC). Results: Chestnut and cedar honey had antiproliferative effects on MCF-10A cells at all doses (1-10 μg/mL), as well as pine honey at the highest dose. However, multifloral honey had no similar effect. Chinese propolis had significant antiproliferative effects on MCF-10A cells at doses of 50-250 μg/mL and on the human periodontal ligament (hPDL) control cells at a dose of 5 μg/mL. Türkiye propolis only had an antiproliferative effect on MCF-10A cells at the highest dose (p = 0.0013). Higher levels of ferulic acid, kaempferol, caffeic acid, pinocembrin and quercetin were detected in Türkiye propolis, while Chinese propolis was rich in pinostrobin. Ferulic acid, pinostrobin and galangin showed antiproliferative properties on MCF-10A cells (p < 0.0001), whereas the remaining four polyphenols had no significant effect on cell viability (p > 0.05). Conclusion: The findings of the study highlight the antiproliferative effects of pinostrobin, ferulic acid and galangin on MCF-10A cells and has also confirmed the antiproliferative effects of honey and propolis samples to be due to their polyphenolic properties. Therefore, this study suggests that polyphenolic substances may have both preventive and therapeutic potential in FBD.
  • Küçük Resim
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    Whole genome expression analysis identifies multiple targeted ıntegrative effects of polyphenol-rich propolis on HER-2-Positive breast cancer cell line
    (ACG Publications, 2024) Seyhan, Mehmet Fatih; Timirci Kahraman, Özlem; Eronat, Allison Pınar; Yılmaz, Eren; Ceviz, Ayşe Begüm; Kısakesen, Halil İbrahim; Öztürk, Tülin; Yılmaz-Aydoğan, Hülya; Öztürk, Oğuz
    Natural products have been focused by researchers due to their important anticarcinogenic characteristics in the treatment of cancer with the slightest side effects possible. Propolis is one of the most prominent candidates among these natural products in terms of its anticancer features. In this study we aim to research the effects of Anatolian propolis on ER/PR-, HER-2/neu+ human breast cancer cell line SK-BR-3 with intent to clarify the molecular mechanism propolis in HER+ breast cancers in overview of whole genomic expression for the first time via a microarray experiment. Afterwards, microarray data was validated via real time PCR with the selected genes. After performing bioinformatic analysis via GeneSpring Software and String analysis, a 50 µg/mL dose of propolis affected several pathways of HER-2 positive breast cancer cells including cell cycle, DNA repair and apoptosis especially at 48th hour exposure. In contrast, after exposure to 50 µg/mL dose of propolis, up-regulated genes were detected at diverse pathways such as immune response, cell migration regulation, organization of cell-cell adhesion, etc. For this reason, we proposed that polyphenol-rich propolis can be used in the treatment of HER-2 positive breast cancer with characteristics of less toxic than the current treatment methods.