Yazar "Tuncel, Ali" seçeneğine göre listele

Listeleniyor 1 - 1 / 1
  • Kapalı Erişim
    Yayın
    Thermo, pH, and ionic-strength-responsive MPEG–PEI copolymer: A reversible soluble–insoluble support promotes glucoamylase immobilization and sustainable catalysis
    (Elsevier, 2026) Haykır, Nazife Işık; Kosaoğlu, Hacim; Tuncel, Ali; Çelebi, Serdar Suut
    Glucoamylase (GA) plays a crucial role in the saccharification of starch and other related oligo saccharides in the food and fermentation industries. The construction of an immobilized GA using a reversibly soluble/insoluble methoxy polyethylene glycol–polyethyleneimine (MPEG–PEI) copolymer synthesized via carbodiimide chemistry is presented in this work. Various MPEG:GA ratios were assessed to optimize enzyme loading and catalytic activity. Immobilized GA retained up to 12.6% of its theoretical activity but demonstrated excellent operational stability and reusability. Substrate-assisted immobilization, using maltose and maltodextrin to protect GA's active site during synthesis of the carrier, significantly improved enzyme activity—up to a 3.1- fold increase compared to the unprotected system. While maltodextrin yielded higher initial activity, maltose with a lower initial activity offered better long-term stability, maintaining 70% of its initial activity even after 11 reuse cycles. Kinetic analysis using the Lambert-W function revealed increased Km for the immobilized enzyme, indicating decreased substrate affinity due to a possible conformational change on the immobilized form of GA. While a minor decrease in conversion efficiency was observed under high initial substrate concentrations in practice, the reversibly soluble/insoluble MPEG–PEI copolymer exhibited effective thermo-responsive char acteristics, enabling the sustained and recyclable use of GA in starch hydrolysis and offering considerable potential for other future biotechnological applications.