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  • Kapalı Erişim
    Yayın
    A dual-drug nanocarrier strategy for ocular fungal infections: Micelles embedded in electrospun nanofibers
    (MDPI Publishing, 2026) Uzel, Egemen; Durgun, Meltem Ezgi; Aydilek, Neriman; Hacıoğlu, Mayram; Güngör, Sevgi; Özsoy, Yıldız
    Ocular fungal diseases are associated with severe infection and pain and, in advanced stages, can lead to vision loss. Current treatment options are limited to the topical ap plication of conventional drugs, and the bioavailability of these drugs is quite limited due to ocular barriers. In this study, a dual-drug nanodelivery system was developed to improve intraocular drug delivery by combining antifungal and anti-inflammatory therapies. Posaconazole (PSC), a broad-spectrum triazole antifungal agent, and dexke toprofen trometamol (DKP), a rapidly acting nonsteroidal anti-inflammatory drug, were co-loaded onto polymeric micelles and then incorporated into electrospun poly(vinyl alco hol)/poly(vinylpyrrolidone) (PVA/PVP) nanofiber intraocular implants. DSC, XRD, FTIR, and FESEM analyses showed that both APIs were successfully converted into nanofiber form without disrupting the micelle structure. Comparative studies with DKP solution and PSC commercial oral suspension (Noxafil® 40 mg/mL) showed that the produced micelle loaded nanofibers provided sustained release and significantly increased ex vivo ocular permeation and penetration. In vitro antifungal activity tests demonstrated efficacy against Candida albicans, and HET-CAM toxicity tests showed that the micelle-loaded nanofibers were non-irritating and suitable for ocular application. Overall, the micelle-loaded electro spun nanofiber ocular inserts developed in this study represent a promising platform for combined antifungal and anti-inflammatory ocular therapy.
  • Küçük Resim
    Yayın
    Preparation and in vitro characterisation of nanofibers for enhancing the water solubility of poorly soluble drugs
    (İstanbul University Press, 2025) Karamürsel, Elif; Uzel, Egemen; Aydilek, Neriman; Durgun, Meltem Ezgi; Özsoy, Yıldız
    Background and Aims: As the drug discoveries of the modern century have led to a rapid increase in the number of new drug candidates with low water solubility, nanofiber drug delivery systems have become a promising technology to increase the water solubility of drugs with a high surface-to-volume ratio. In this study, we aimed to prepare a nanofiber of a molecule with low water solubility and investigate its changing solubility properties. Methods: Three nanofiber dosage forms containing olanzapine (OLZ) active substance were developed by the electrospinning method using polyvinyl alcohol (PVA) polymer. Drug loading efficiency, zeta potential determination, electrical conductivity, rheology, field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analyses were performed to evaluate the in vitro characterisation of the formulations. The solubility profile of the optimised formulations in pH 7.4 phosphate buffer was evaluated. The stability of optimised formulations was evaluated in terms of physical properties (colour, shape, weight, diameter, and thickness) and drug amount for 35 days. Results: It was determined that the electrospinning property of the nanofiber preparation solution increased with the addition of ethanol to the polymer solvent medium. The active substance distribution in the nanofiber layer was more homogeneous in the N78 and N79 coded formulations with high zeta potential values compared to N69. Contrary to the homogeneous distribution problem, the loading efficiency of the N69-coded formulation containing chloroform (~29%) was higher than that of N79 (~9.8%). A 24-h solubility study in pH 7.4 phosphate buffer of the N78-coded formulation, which has an active ingredient loading efficiency of ~80.4%, confirmed the increased solubility of OLZ in water in the nanofiber drug delivery system. Conclusion: Further studies are needed to convert these model formulations into final drug products.