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Utilization and fertility preservation outcomes in women undergoing embryo cryopreservation before breast cancer treatment: A meta-analysis
(Elsevier, 2025) Turan, Volkan; Öktem, Özgür; Bang, Heejung; Oktay, Kutluk Han
Our aim was to assess fertility preservation (the proportion of women who had at least 1 live birth) and utilization rates (the proportion of women who utilized their cryopreserved embryos) rates among women who cryopreserved their embryos before breast cancer treatments. PubMed and Cochrane library database were searched until December 2024. We included all studies that reported pregnancy outcomes, the number of women who returned for frozen embryo transfer, and the number of women who preserved their fertility. The primary outcome measures were the utilization and fertility preservation rates. Secondary outcome measures were implantation, clinical pregnancy, and live birth rates. Of the 12 studies, 9 met the criteria, encompassing >2126 women with breast cancer who cryopreserved their embryos for fertility preservation. In 9 studies that reported the total number of attempts, 424 women underwent 863 embryo transfers. Based on those studies, the clinical pregnancy and live birth rates were 50% (95% CI: 35-65, I 2: 80%) and 33% (95% CI: 22-46, I 2: 76%), respectively. The utilization and fertility preservation rates were 18% (95% CI: 9-32, I 2: 95%) and 39% (95% CI: 29-51, I 2: 48%), respectively, all from random-effects models. Fertility preservation success with embryos cryopreserved before breast cancer treatments seems to be promising. However, the utilization rate of cryopreserved embryos is low. Additional studies with larger sample size and longer follow up are required to evaluate the long-term utility rates.
Child and adolescent psychiatry consultations during the COVID-19 pandemic
(Springer Nature Link, 2025) Vatansever Pınar, Zeynep; Çimen, İrem Damla; Küçük, Elif; Köle, Mehmet Tolga; Kandemir, İbrahim
This study aimed to identify the biopsychosocial stressors influencing child and adolescent mental health during periods when facetoface education was suspended and to evaluate the psychological sequelae of school closures and related restrictions. We conducted a retrospective observational study at Kartal Dr. Lütfi Kırdar City Hospital, a tertiary care center in İstanbul, reviewing all patients referred to the child and adolescent consultation-liaison psychiatry (CLP) unit between March 2020 and March 2022. We assessed associations between psychiatric diagnoses and health-related stressors, school closure status, age, and sex using both frequentist and Bayesian methods. During the study period, 264,013 pediatric admissions were recorded, of whom 270 (0.10%) required psychiatric consultation. The proportions of suicide attempts and anxiety diagnoses did not differ between periods of open and closed schools (BF10 = 0.21 and 0.138, respectively; moderate evidence for the null). Multivariate analysis showed that the odds of suicide attempts were higher in patients exposed to family or schoolrelated stressors (OR = 6.63, 95% CI 2.72–16.19), in females (OR = 8.10, 95% CI 4.16–15.77), and with increasing age (OR = 1.32 per year, 95% CI 1.16–1.50). Female sex (OR = 4.17, 95% CI 2.03–8.55) and older age (OR = 1.30 per year, 95% CI 1.12–1.50) were also associated with depression. Anxiety was more likely in those facing isolation or healthrelated stressors (OR = 3.91, 95% CI 1.66–9.22). These findings highlight the differential impact of stressor type on internalizing symptoms and may inform resource allocation and crisisresponse planning in child mental health services.
Comprehensive classification of variations of the anterior part of the circle of willis in fresh cadavers anterior communicating artery
(Galenos Publishing House, 2025) Nteli Chatzioglou, Gkionoul; Nas, Emine; Kale, Ayşin; Aktaş, Kardelen; Coşkun, Osman; Gayretli, Özcan
Objective: The goal of our study is to evaluate and classify the variations of the anterior communicating artery (AComA) on fresh cadavers from the Türkiye population. Methods: In this study, 182 fresh cadavers were analysed and classified according to the number, shape and course of the AcomA. Results: In our study, typical AcomA was the most common with a rate of 86 (47.25%), while variations of the AcomA were found in the remaining 96 (52.75%) cases. Among these variations, in 11.46% (11/96) of cases, AcomA variations were identified as distal and proximal duplications according to the number of branches they represented; 68.75% (66/96) of cases were identified by their shape (X-shaped, single/double fenestration, hypoplasia, or aplasia); and, in 19 cases, it was characterized by course (median artery or oblique course). The rate of variations was 65% (26/40) in females and 49.29% (70/142) in males. In our study, the X-shaped and single fenestration variations were recorded as the most common. Conclusions: The results of the study are important for cerebrovascular surgery and radiological interventions. It emphasises the importance of recognising and considering variations. The study will contribute to the understanding of cerebrovascular diseases and the development of treatment strategies.
Psychological impact of the February 6 earthquake on displaced individuals with proximal loss residing in temporary shelters
(Routledge, 2025) Filazoğlu Çokluk, Gülşen; Bal, Fatih; Savaş, Esra
The present study examines the psychological effects of the earthquake that occurred in Turkey on 6 February 2023 on individuals residing in temporary shelters who have experienced losses in their immediate surroundings. The objective of the present research is to elucidate the relationships between post-traumatic stress disorder (PTSD), depression, anxiety, and the subjective impact of the traumatic event. Nine months following the disaster, a survey was conducted among 923 adults aged 25 to 60 residing in temporary shelters in Antakya. Participants completed a series of validated and reliable measurement tools, including the DSM-5 Post-Traumatic Stress Disorder Checklist (PCL-5), the Beck Anxiety Inventory (BAI), the Beck Depression Inventory (BDI), and the Impact of Events Scale (IES). The findings indicate significant and positive correlations between PTSD and all other variables, including the impact of the event, depression and anxiety. The multiple regression analysis revealed that these three variables significantly predicted PTSD symptoms and collectively explained 41% of the variance in PTSD levels. This study emphasizes the cumulative psychological impact of forced displacement and close losses on individuals and underscores the pressing need for culturally sensitive and trauma-informed mental health services in post-disaster intervention processes. The findings contribute to the understanding of trauma dynamics in post-disaster communities and guide the development of targeted mental health policies and psychosocial support programs.
Predicting risk of lymph node metastasis in papillary thyroid cancer using miRNAs and clinicopathological features
(Springer Nature Link, 2025) Ergün, Penbe Gül; Aydoğan, Çağatay; Ceviz, Ayşe Begüm; Candan, Gonca; Batur, Şebnem; Yılmaz Aydoğan, Hülya; Öztürk, Oğuz; Öztürk, Tülin
This study investigates potential prognostic values in models created with clinico pathological features and expression profles of miRNAs known for their critical roles in papillary thyroid carcinoma (PTC) for lymph node metastasis (LNM) in PTC cases. Forty-seven lymph node-metastatic PTC patients and 46 non-metastatic patients were included. Using RT-PCR, miR-21, miR-146b, miR-221, and miR-222 expression was analyzed in carcinoma tissues and metastatic lymph nodes of the same PTC patients and carcinoma tissues of non-metastatic PTC patients. MiR 146b (p<0.001) and miR-221 (p=0.009) were overexpressed in metastatic PTCs compared to non-metastatic PTCs. The miRNA expression profles were similar in the lymph nodes and primary cancer of metastatic PTCs. In the presence of larger tumors (>1 cm) in metastatic PTCs, miR-146b and miR221 were overexpressed in the tumor tissue (p=0.036), while miR-222 was overexpressed in metastatic lymph nodes (p=0.035). miR-146b was also upregulated in lack of peritumoral lympho cyte infltration (p=0.006). In conclusion, our fndings suggest that the overexpres sion of miR-146b and miR-221 in PTC tissues may be associated with lymph node metastasis and a poorer prognosis. Furthermore, the presence of larger tumors and increased levels of intratumoral fbrosis in non-metastatic PTC patients could indi cate a poor prognosis.
Antihypertensive treatment of a patient with normal blood pressure: Case report and call for paying attention
(Wolters Kluwer, 2025) Atmaca, Hasan; Erol, Mustafa Kemal; Yetkin, Ertan
The current 2024 European Society of Cardiology (ESC) guideline for the management of elevated blood pressure and hypertension defines blood pressure less than 115/65 mmHg by ambulatory blood pressure monitoring (ABPM) as nonelevated, blood pressures in-between 115–129 and 65–79 mmHg as elevated blood pressure, and hypertension as ≥130/80 mmHg. There, might be patients seeking medical attention for the symptoms, apparently nonspecific but suggestive of hypertension with optimal, or not elevated, or elevated blood pressure values. A female patient with complaints of headache and dizziness lasting for 2 months has been evaluated in cardiology outpatient clinic and assessed by ABPM. It has been told that she had previously blood pressure of 90–100/50–60 mmHg and was suffering from headache when systolic blood pressure exceeds 110 mmHg. Her 24-h ABPM revealed systolic and diastolic blood pressure as 106/63 mmHg showing nighttime decrease compared with daytime pressures (98/59 mmHg and 108/68 mmHg, respectively). Thereafter, she was instructed to keep continuing the life-style modification and given to beta-blocker (bisoprolol 5 mg) as an antihypertensive treatment. At the end of the 2 weeks of follow-up period, she was headache-free and was feeling comfortable and well with a mean home blood pressure of 98/56 mmHg. We have presented prosperous antihypertensive treatment of a female patient suffering from headache and dizziness with a numerically normal or nonelevated blood pressure. In the presence of symptoms and having not elevated or elevated blood pressure levels, patients’ history on previous measure of blood pressure might facilitate our decision-making process.
Comprehensive analysis of brewed tea: Boron content, total antioxidant and oxidant capacity, oxidative stress, and ph value
(Springer Nature Link, 2025) Cihan, Aslıhan; Özel Korlu, Armağan Begüm; Tüzüner, Burcin Alev; Yarat, Ayşen
Tea is widely recognized as the most consumed beverage in the world after water. In recent years, the connection between tea and health has gained growing attention as a signifcant research topic. In this study, we evaluated the relationship between tea and health by analyzing the boron content, total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), and pH levels of teas commonly consumed in Türkiye. A total of 42 samples were created from various tea brands and diferent tea types that are widely consumed in Türkiye. The determination of boron in pulverized and brewed tea samples was carried out using the carminic acid method. Additionally, boron transfer was determined in brewed teas. Brewed tea samples were also examined in terms of pH, TAC, TOC, and OSI. It was determined that all teas contained an average of 11.73 mg/L boron, the amount of boron in domestic teas was lower than in imported teas. It was determined that boron transfer into brewed tea was approximately 30%. The pH values ranged between 4–5. No diferences were detected in terms of TAC, TOC, and OSI. Our research shows that tea consumption is safe in terms of boron levels and helps meet daily needs. The OSI of teas is less than 1, so their antioxidant efects can be considered quite good. However, it should be noted that excessive tea consumption can lead to the demineralization of tooth enamel due to low pH levels.
In-depth analysis of the demographic landscape and clinical outcomes of assisted reproductive technologies in Türkiye: A comprehensive survey for the years 2020 and 2021
(Galenos Publishing House, 2025) Benlioğlu, Can; Aydın, Yunus; Bostancı, Mehmet Sühha; Bozdağ, Gürkan; Bulgurcuoğlu, Sibel; Demir, Ahmet; Dilbaz, Serdar; Dirican, Enver Kerem; Turan, Volkan; Balaban, Başak
Objective: To investigate patient characteristics and outcomes of assisted reproductive technology (ART) cycles conducted in Türkiye during the coronavirus disease-2019 (COVID-19) pandemic (2020-2021). Material and methods: ART centers in Türkiye were invited to participate in this survey. The questionnaire focused on patient demographics and performance outcomes and was sent to center directors via anonymous Qualtrics™ links. Results: The survey was sent to 167 centers and data were collected from 15 centers in 2020 and 24 centers in 2021. The clinical pregnancy rate from intrauterine insemination (IUI) procedures remained similar, with 15.1% in 2020 (1,245 IUI cycles) and 14.5% in 2021 (2,023 IUI cycles), while successful delivery rates were 12.5% and 11.5%, respectively. For ART treatments, the clinical pregnancy rate from fresh embryo transfers increased from 45.1% in 2020 (3,119 transfers) to 50.4% in 2021 (8832 transfers), with similar live birth rates, 34.2% vs. 34.4%. Frozen embryo transfers resulted in clinical pregnancy rates of 47.8% in 2020 (2,498 transfers) and 51.9% in 2021 (12,015 transfers), with live birth rates rising from 39.8% to 42.7%. Preimplantation genetic testing cycles resulted in clinical pregnancy rates of 64.2% in 2020 (271 transfers) and 60.8% in 2021 (2,102 transfers), with live birth rates of 53.5% and 48.2%, respectively. Regarding techniques for fertility preservation in females, 11 prepubertal and 61 postpubertal ovarian tissue cryopreservation procedures were reported, alongside 1,346 cycles performed within the same period. In males, 144 post-pubertal testicular tissue, 871 epididymal and 2,480 ejaculated sperm cryopreservations were reported. During the two years, six ovarian tissue transplantations followed by ART were performed, with 96 women using cryopreserved oocytes. In addition, 40 testicular tissue, 298 epididymal, and 238 ejaculated sperm samples were used for ART purposes. Conclusion: This survey provides a comprehensive overview of ART practices in Türkiye for 2020 and 2021, establishing a long-term, nationallevel analysis while highlighting the challenges posed by the COVID-19 pandemic. The longitudinal analysis established a foundation for future annual reports and offers critical insights into emerging trends over these two years.
CYB5R4 gene methylation as a potential epigenetic marker for ovarian cancer
(SAGE Publications, 2025) Gönç, Ayşegül; Şükrüoğlu Erdoğan, Özge; Kılıç Erciyas, Seda; Çelik Demirbaş, Betül; Dinç, Ahmet; Pasin, Özge; Saip, Pınar; Yazıcı, Hülya; Tunçer, Şeref Buğra
Background: Ovarian cancer (OC) is a significant health problem often diagnosed at an advanced stage due to the lack of early symptoms and effective screening methods. This study aimed to explore the role of CYB5R4 gene methylation as a potential biomarker for ovarian cancer. Methods: DNA isolation was performed in the blood samples of 387 ovarian cancer patients, 50 individuals with benign ovarian diseases, and 100 healthy controls. The CYB5R4 gene methylation status was evaluated using the Methyl-Specific Restriction Enzymes (MSREs) technique and methylation levels were compared between the groups. Results: Ovarian cancer patients exhibited the highest mean methylation percentage (9.45%) and median (6.23%), followed by healthy controls with a mean of 9.14% and a median value of 4.47%. Statistical analysis showed significant differences in methylation levels (P=.041), suggesting that CYB5R4 methylation may be associated with ovarian cancer progression. Conclusion: The CYB5R4 gene methylation may serve as a potential biomarker for ovarian cancer, particularly in distinguishing between malignant and benign conditions. Further research is needed to validate these findings and explore the clinical utility of CYB5R4 methylation in ovarian cancer management.
Anatomy based computer assisted evaluation for aesthetic new navel formation
(Springer Nature Link, 2025) Gövsa, Figen; Nteli Chatzioglou, Gkionoul; Biçer, Ahmet; Gökmen, Gökhan
Background This study aimed to determine the typical position and shape of the umbilicus among young individuals to guide the repositioning of the new umbilicus during abdominoplasty. Methods Standardized photographs of 63 young volunteers were analyzed to evaluate parameters such as the vertical and horizontal positions, length, ratio, and shape of the umbilicus. The study also established the mathematical relationships between the umbilicus and nearby anatomical structures like the xiphoid process, pubic symphysis, anterior superior iliac spine, iliac crests, and body mass index (BMI) to identify the ideal geometric shapes and positions. In two patients with abdominal skin sagging, abdominoplasty was performed with a new umbilicus designed according to these anatomical measurements. Results The umbilici were categorized by shape: vertical oval with superior hooding (29%), round (27%), vertical lozenge (21%), and protruded (11%). Ratios between anatomical points were measured, such as the ratio of the distance between the xiphoid process and the upper edge of the umbilicus to the distance between the lower edge of the umbilicus and the sym physis pubis, which was 1.64±0.40. Post-operative evaluation emphasized achieving a xiphoid-umbilicus/pubic symphysis ratio of 1.6:1 for the new umbilicus, with an aesthetically ideal ratio considered to be 1:1. A strong correlation was found between BMI and all umbilical ratios. Conclusions The most aesthetically pleasing navel position aligns with the golden ratio between xiphoid and umbilicus compared to the umbilicus and abdominal crease. Weight control is also crucial in achieving optimal belly aesthetics.
An analytical method for the determination of pentachloroaniline and pentachlorobenzene in ginseng tea samples by gas chromatography–mass spectrometry after liquid phase microextraction
(Elsevier, 2025) Erarpat Bodur, Sezin; Serbest, Hakan; Bakırdere, Sezgin
This study describes a sensitive and accurate analytical method for the determination of pentachloroaniline (PCA) and pentachlorobenzene (PCB) at trace levels in ginseng tea samples. For this purpose, spraying based fine droplet formation liquid phase microextraction (SFDF-LPME) method was implemented to extract/preconcentrate the target analytes before their separation and detection by gas chromatography-mass spectrometry (GC-MS). Herein, a lab-made spraying system was used to distribute the extraction solvent throughout the aqueous sample solution. Limit of detection/quantitation (LOD/LOQ) values for PCA and PCB were found as 0.24/0.80 and 0.26/0.85 μg/kg, respectively. When LOD values of GC-MS and SFDF-LPME-GC-MS methods were compared to each other, enhancement in detection power values for PCA and PCB were 285 and 226.9 folds, respectively. Percent recovery results for PCA and PCB were calculated as 92-124 % and 86-129 %, respectively. Green evaluation and practicability of developed SFDF-LPME-GC-MS method was done by Eco-scale (89), AGREEprep (0.39) and BAGI (62.5) tools.
From surgery to sports career: The long-term fate of athletes with discoid meniscus
(Lippincott Williams and Wilkins Ltd., 2025) Erden, Tunay; Ağır, Muzaffer; Ali, Jotyar; Toker, Berkin; Taşer, Ömer
Objective: This study aimed to evaluate the long-term effectiveness of different surgical techniques for discoid meniscus in athletes. Design: This retrospective cohort study included 44 athletes (51 knees) who underwent arthroscopic surgery for symptomatic discoid meniscus between 1996 and 2019. Setting: The study was conducted at a tertiary-level sports medicine center. Patients: Athletes aged 15 to 35 years with symptomatic discoid meniscus confirmed by magnetic resonance imaging and requiring surgical treatment were included. Exclusion criteria were cartilage injuries (Outerbridge grades 3-4), concomitant ligament injuries requiring surgery, osteochondritis dissecans, and prior knee surgery. Interventions: Patients underwent arthroscopic partial, subtotal, or total meniscectomy, with or without meniscus repair. Main outcome measures: Functional outcomes were assessed using Visual Analog Scale (VAS), International Knee Documentation Committee (IKDC), and Lysholm scores. Osteoarthritis progression was evaluated using the Kellgren-Lawrence classification. Return to sports time and total career duration were also recorded and compared between the surgical groups. Results: The mean follow-up was 13.5 ± 6.2 years (range: 5-27 years). At the final follow-up, 72% of the meniscus-preserving group (partial meniscectomy and repair) had KL grade 0 osteoarthritis, whereas 38% of the subtotal/total meniscectomy group developed KL grades 3 to 4 osteoarthritis (P < 0.001). All groups showed significant postoperative functional improvements (P < 0.001), but career duration and return to play time were significantly longer in the meniscus-preserving group (P = 0.004, P < 0.001, respectively). Conclusions: Subtotal/total meniscectomy significantly accelerates osteoarthritis progression. Meniscus-preserving techniques provide better long-term outcomes.
Osseous anatomy of the distal radius: A morphometric analysis of external groove and bony ridge
(Springer Nature Link, 2025) Ok, Fatma; Çelikgün, Beyza; Nteli Chatzioglou, Gkionoul; Ertaş, Ahmet; Coşkun, Osman
Purpose: This study aimed to investigate the presence and morphological and morphometric characteristics of the distal radius's external groove (EG) and bony ridge, which play a potential role in the pathogenesis and treatment difficulties of De Quervain's tenosynovitis. Methods: The osseous anatomy of the distal radius was analyzed in detail on a total of 103 dry bone specimens (49 left and 54 right) obtained from the Department of Anatomy, Istanbul University, Turkiye. The frequency and morphology of the EG were analyzed. The EGs were categorized as Type 1, Type 2, and Type 3 with two EGs, one EG with depth, and an additional EG without depth, respectively. Finally, flat areas without clear groove boundaries were classified as Type 4. Results: The analyses showed variations in the morphology and dimensions of the EG and bony ridge. A total of 110 EGs were detected in 103 radii examined. Type 1, Type 2, Type 3 and Type 4 EGs were reported in 30 bones (30%), 38 bones (38%), 12 bones (12%) and 23 bones (23%), respectively. The depth of the EG of Type 1 was 0.26 ± 0.23 mm and 0.22 ± 0.16 mm on the lateral and medial sides, respectively. Also, the depth of the Type 2 was calculated as 0.49 ± 0.37 mm. Conclusion: The osseous variations of the distal radius are critical anatomical factors contributing to the pathogenesis of De Quervain's tenosynovitis and difficulties in the treatment process. It is essential to recognize these variations to improve diagnostic accuracy and optimize therapeutic interventions.
Variations in the course of the external branch of the superior laryngeal nerve and their importance in surgical procedures: An experimental cadaveric study
(The Polish Otolaryngology, 2023) Nteli Chatzioglou, Gkionoul; Sarı, Elif; Gayretli, Özcan; Coşkun, Osman; Öztürk, Adnan; Gürses, İlke Ali
Introduction: Injury of the external branch of the superior laryngeal nerve can cause a hoarse or weak voice due to the functional loss (dysergia) of the cricothyroid muscle. Defining the anatomical variations of the external branch of the superior laryngeal nerve and estimating their frequency are crucial for surgical interventions. Aim: To reveal the topography of the external branch in the Anatolian population, to prevent injury of it during the surgical intervention in the anterior neck region. Material and methods: 26 bilateral hemilarynges (4 females, 22 males) were dissected. The morphometric and morphological features of the external branch were examined. The obtained results were compared statistically, left and right. Results: Landmarks such as the thyroid gland and laryngeal prominence were determined for the detection of the external branch. The variations of the course of the external branch and the points of piercing the cricothyroid muscle or inferior pharyn geal constrictor muscle were evaluated. Discussion: Although safe approaches have been described for nerve protection during neck surgeries, injuries may occur during preliminary surgery as the mentioned nerve is thinner and more superficial than other branches of the vagus nerve. However, it can be detected more easily and safely by knowing the defined anatomical landmarks and morphological varia tions of the external branch. Conclusion: The anatomical variations described can be a safe and important guide in surgeries of the anterior neck region.
Fluid biomarkers in atypical Parkinsonism: Current state and future perspectives
(Springer Nature Link, 2025) Bougea, Anastasia; Colosimo, Carlo; Falup‑Pecurariu, Cristian; Palermo, Giovanni; Değirmenci, Yıldız
Diagnosing Atypical Parkinsonian Syndromes (APS) may be challenging due to overlapping clinical features of Parkinson’s disease (PD), and the lack of pathognomonic diagnostic tests. Fluid biomarkers can be useful tools that make it easier to identify and track diferent APS. Objectives: this narrative review aim to update the current state of fuid biomarker research in APS and their potential implications in clinical practice. A comprehensive literature search was conducted in PubMed and Scopus using the following terms: “Aβ42 amyloid beta with 42 amino acids’’, “ alpha-synuclein’’, “Atypical Parkinso nian Syndromes’’, “corticobasaldegeneration’’, “C reactive protein’’, “cerebrospinal fuid’’, “dementia with Lewy bodies’’, “multiple system atrophy’’, “neuroflament light, oligomericαsyn, phosphorylated α –syn’’, “tau phosphorylated at threonine 181’’, “progressive supranuclear palsy’’, “Seeding Amplifcation Assay’’, “t-tau; total tau”. The lack of high-afnity α-syn antibodies and ligands may contribute to α-syn’s low efcacy as a diagnostic biomarker of APS. Cerebrospinal fuid (CSF) biomarkers refecting Alzheimer pathology, axonal damage (neuroflament light chain) add valuable diagnostic and prog nostic information in the neurochemical characterization of APS. Infammatoryand microRNAs markers need to be further validated before their clinical use. Seeding Amplifcation Assays (SAA), despite their high sensitivity and specifcity, are at this point used only as a research tool, and they are not quantitative or refective of disease severity. Biomarker research for early identifcation and prognosis of APS patients requires multicenter collaboration, validation, and AI-based diagnostics, despite immature biological classifcation systems.
Protective effects of boric acid on HBV-transgenic mice with chronic alcohol consumption: An experimental study
(Elsevier, 2025) Ergüven, Pelin; Tanrıkulu Küçük, Sevda; Şevgin, Kübra; Değirmencioğlu, Sevgin; Çetinalp, Pınar; Aksu, Soner; Gün Atak, Palmet; Söğüt, İbrahim
This study aimed to investigate the protective effect of boric acid supplementation against liver damage in chronic alcohol-dependent HBV transgenic mice. The HBV transgenic mice were divided into four groups: control (C), boric acid(B), alcohol(A), and alcohol + boric acid(A + B). Blood alcohol concentration (BAC), alanine aminotransferase (ALT), aspartate aminotransferase(AST), reactive oxygen species(ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (TAS), total oxidant capacity (TOS) levels, and oxidative stress index (OSI) were examined biochemically. H&E, PAS, Masson trichrome, and TUNEL staining were performed. Caspase 3, cytochrome c, and APAF-1 expression levels were determined by qRT-PCR. The alcohol group exhibited significantly higher levels of ROS, MDA, TOS, OSI, and mRNA expressions of Cytochrome c, caspase 3, and APAF-1, while TAS level and CAT activity were significantly lower compared to the boric acid group. Compared to the control group, the alcohol group exhibited significantly increased TOS, OSI, AST levels, APAF-1 mRNA expression, and the number of TUNEL-positive cells, along with a reduction in GPx activity (p < 0.05). However, in the alcohol + boric acid group, TOS and AST levels were significantly higher compared to the control group (p < 0.05), and TOS was higher compared to the boric acid group (p < 0.01). Among the boron-treated groups, only the TOS level was lower in the boric acid group compared to the alcohol + boric acid group (p < 0.01). Histopathological examination revealed reduced sinusoidal dilatation and connective tissue distribution in the boric acid-supplemented groups. These findings suggest that boric acid supplementation may mitigate oxidative damage and histopathological alterations associated with chronic alcohol consumption in HBV-transgenic mice.
Design and synthesis of thiosemicarbazides and 1,2,4-triazoles derived from ibuprofen as potential MetAP (type II) inhibitors
(Elsevier, 2025) Yılmaz, Özgür; Biliz, Yağmur; Ayan, Sümeyra; Çevik, Özge; Karahasanoğlu, Müfide; Çotuker, Reyhan; Mert Şahin, Naz Mina; Gökkaya, Kübra; Gülyüz, Sevgi; Yelekçi, Kemal; Küçükgüzel, Ş. Güniz
In the present study, a range of novel thiosemicarbazides 4a-i and 1,2,4-triazoles 5a-i derived from ibuprofen, were synthesized. Structural elucidation of these synthesized compounds was performed utilizing a variety of spectroscopic methods, including FTIR, 1 H NMR, 13C NMR and HR-MS. The synthesized compounds were tested for cytotoxicity in five different cancer cell lines (cervical cancer (HeLa), human breast cancer (MCF-7), human gastric adenocarcinoma (MKN-45), human metastatic prostate cancer (PC3) and human glioblastoma (U87)). The compounds were compared with healthy cells (NIH-3T3) and the most effective compounds were determined by means of the selectivity index. Thiosemicarbazides derived form ibuprofen 4i and 4d showed anticancer activity, while 1,2,4-triazoles derived form ibuprofen 5b, 5c, 5d, 5e, 5h, 5g showed anticancer activity in HeLa, MCF-7, MKN-45, PC3 and U87 cells. To test the stability of the protein-drug complexes all 18 compounds 4a-i and 5a-i were docked into the active site of the MetAP2 enzyme In general, computational inhibition constants values were correlated with the experimental values. The dynamic behavior of MetAP2-inhibitor complexes was analyzed using all atoms Molecular Dynamic (MD) simulations for 200 ns duration. MD revealed that the drugs bind in the active center of MetAP2 with stable RMSD and RMSF. In conclusion, in-silico results and in-vitro studies suggests that thiosemicarbazides and 1,2,4-triazoles derived from ibuprofen may be novel anticancer drug candidates for treating cervical, breast, prostate, gastric and glioblastoma. Compounds provided induction of apoptotic proteins in the cell by inhibiting MetAP2 enzyme. Furthermore, the potential antioxidant activities of the compounds were evaluated using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. Among the compounds tested, 4a, 4b, 4e, 4f, 4h, and 4i exhibited values closely resembling the DPPH activity of the standards.
Cytotoxic and anti-migratory effects of polyphenolic compounds on breast cancer cells by altering Jam-A, LFA-1, and VLA-4 gene expression
(Taylor & Francis, 2025) Özkara, Gülçin; Ceviz, Ayşe Begüm; Eronat, Allison Pinar; Pehlevan Karabıyık, Funda; Candan, Gonca; Özturk, Oğuz; Yılmaz Aydoğan, Hülya
This study represents the initial research of the effects of a com bination of the largest number (13) of different polyphenic sub stances (PFK5120), formulated based on the propolis content on cell viability, migration and expression of lymphocyte function-associated antigen-1 (LFA-1), very late antigen-4 (VLA-4) and junction adhesion molecule A (Jam-A) in breast cancer (BC) cells. PFK5120 negatively affected cell viability at a 5% concentra tion as compared with unexposed ones (p<0.001). Treatment with 20% PFK5120 for 48h down-regulated Jam-A in MCF-7 and MCF-10A, up-regulated LFA-1 in MCF-10A and MDA-MB-231, and down-regulated VLA-4 in MCF-10A and MDA-MB-231 (p<0.001). Furthermore, migration was found to be inhibited by PFK5120 at varying doses and times. Migration was completely inhibited by 35% PFK5120 treatment in MDA-MB-231, while even lower concen trations (10%) were effective in MCF-7. Current findings indicate that PFK5120 represents a valuable natural component of BC ther apy through its cytotoxic and anti-migratory effects.
The validity and reliability of the Turkish version of the patient’s knee implant performance (PKIP) questionnaire-preoperative and postoperative
(Wolters Kluwer Healt, 2025) Şahan, Nilay; Atasavun Uysal, Songül; Parmaksız, Ayhan; Aydın, Erbil
The Patient’s Knee Implant Performance (PKIP) Questionnaire is a short and easy-to-complete questionnaire developed to assess the performance of total knee arthroplasty (TKA) more comprehensively. The aim of this study was to investigate the validity and reliability of the Turkish version of the PIKP questionnaire before (PKIP PreOp) and after (PKIP PostOp) TKA. The study included 162 patients referred for TKA and 154 patients who had undergone the surgery at least 3 months prior. Cronbach alpha, intra class correlation coefficient, and item-total correlation values were calculated to assess the reliability of the PIKP questionnaire. Validity was determined using exploratory and confirmatory factor analysis. To determine parallel scale validity, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), The Short Form-36 (SF-36) Quality of Life Scale performance score were used. The mean age of the participants was 66.39 ± 7.65 years. Cronbach alpha and intra-class correlation coefficient values of the PKIP were acceptable (0.723 and 0.985, respectively). The item-total correlation values of each item of the PKIP was also acceptable (lowest ranged from 0.335 to 0.621). Confirmatory and exploratory factor analysis revealed that the both PKIP PreOp and PKIP PostOp studies had sufficient fit. The PKIP PreOp and PKIP PostOp was moderately to strongly correlated with the Western Ontario and McMaster Universities Osteoarthritis Index and Short Form-36 score (P < .001). Patients undergoing TKA had a significantly higher PKIP PostOp score than PKIP PreOp score. The Turkish version of the PKIP is valid, reliable, and sensitive to assess in performance in patients undergoing TKA.
Nanodelivery approaches of phytoactives for skin cancers: Current and future perspectives
(Bentham Science Publishers, 2025) Algın Yapar, Evren; Özdemir, Merve Nur; Durgun, Meltem Ezgi; Akbal Dağıstan, Özlem; Cavalu, Simona; Özsoy, Yıldız; Kartal, Murat
In recent years, there has been an increase in skin cancers due to external factors, especially environmental factors, and studies on treatment alternatives have gained importance. Nanomaterials are common, from sunscreen formulas to formulations designed to treat skin cancers at various stages. Using bioactives has multiple effects in treating skin cancers, which provides many advantages. In this regard, many phytochemicals gain importance with their antioxidant, anti-proliferative, anti-inflammatory, antiangiogenic, and analgesic effects. Their delivery with nanocarriers is on the agenda for phytochemicals to gain the targeted stability, effectiveness, and toxicity/safety properties. This review presents types of skin cancers, phytochemicals effective in skin cancers, and their nanocarrier-loaded studies from an up-to-date perspective.

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