İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
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Güncel Gönderiler
Biocompatibility of printed, milled, and conventionally fabricated indirect resin-based restorative materials: An in vitro comparative study
(Springer Nature Link, 2026) Atay, Ayşe; Ayvalıoğlu Şamiloğlu, Demet Çağıl; Şişmanoğlu, Soner; Yıldırım, Zafer; Üşümez, Aslıhan
The increasing use of digitally fabricated resin-based materials for definitive dental restorations has raised concerns regarding their biological performance and biocompatibility. This in vitro study evaluated the biocompatibility and surface characteristics of different indirect resin-based restorative materials used in definitive dental restorations. A total of 144 disc-shaped specimens (diameter 15 mm, height 2 mm) were fabricated using printed, milled, and conventional resins (48 specimens per material), with 12 specimens allocated to each analysis (n=12). Specimens were sterilized using ultraviolet irradiation. Culture medium extracts were obtained on days 1, 3, and 7 and evaluated using L929 mouse fibroblast cells. Cell viability was assessed using the XTT assay, apoptosis by Annexin V-FITC/PI staining, and interleukin-6 (IL-6) release using a Mouse IL-6 ELISA kit. Cell viability, apoptosis, and IL-6 levels were analyzed using two-way ANOVA; surface roughness was analyzed using one-way ANOVA, followed by Tukey’s HSD post hoc tests (p<0.05). Significant differences were observed among the manufacturing groups across incubation periods in terms of cell viability, apoptosis rate, and IL-6 release (p<0.05). The milled-resin group showed the highest cell viability on day 7, whereas the conventional resin group showed the lowest (p<0.05). On days 3 and 7, the conventional resin group exhibited the highest rates of apoptosis and IL-6 release (p<0.05). Both printed and milled-resin groups showed no significant impact on fibroblast cell responses, whereas exposure to eluates from the conventional resin group resulted in altered cell viability, IL-6 levels, and apoptosis, suggesting potential cytotoxicity relevant to material selection for definitive prosthodontic restorations.
Selective effect of atmospheric non-thermal plasma on resin cement shear bond strength to glass ceramics
(BioMed Central, 2026) Mustafaoğlu, Emine; Ercan, Utku Kürşat; Özdemir, Gizem Dilara; Bağış, Bora
Statement of problem The effectiveness of non-thermal plasma (NTP) in improving the bond strength of different resin cements to different glass ceramics under various surface treatment protocols remains unclear. Moreover, the influence of specific plasma devices and the material-dependent response of ceramic–resin cement systems to NTP have not been sufficiently investigated. Purpose The purpose of this in vitro study was to evaluate the effect of NTP on the shear bond strength of different resin cements to different glass ceramics by comparatively assessing multiple surface treatment protocols. Materials and methods A total of 240 specimens of each glass ceramic group (IPS e.max CAD (E), Vitamark II (V)) were divided into 24 groups according to the combination of 6 surface treatments ((control), hydrofluoric acid (HF)+ceramic primer (Pr), Pr, HF+plasma (Pl) + Pr, Pl + Pr, and Pl) and two different resin cements (Single Bond+RelyX Ultimate (R) and ZenitCem (Z)). NTP was applied with an AC microsecond pulse power supply (5 kHz, 20 kV, 90 s). Specimens were cemented to composite cylinders. After storage in an oven, SBS was measured. The results were analyzed using three-way ANOVA, post-hoc tests, and targeted pairwise comparisons, with the significance level set at α = 0.05. The effectiveness of NTP is material- and cement-dependent and should be considered as a selective surface modification strategy rather than a universal alternative to conventional surface treatments. Results The V-Pl-Z group showed significantly higher SBS values than the V-Z group, and these values were within the clinically acceptable range (p < 0.05). In the V groups treated with HF + Pr and HF + Pl+Pr, R demonstrated higher bond strength values than Z. HF surface treatment increased the SBS values for all groups (p < 0.05). Conclusions The effectiveness of NTP is material- and cement-dependent and should be considered as a selective surface modification strategy rather than a universal alternative to conventional surface treatments Clinical significance This study demonstrates that atmospheric non-thermal plasma does not universally enhance ceramic–resin cement bonding. Its effectiveness depends on the ceramic substrate, resin cement type, and surface treatment protocol. Clinicians should therefore apply plasma selectively.
Integrating green chemistry principles into the fabrication of MIP electrochemical sensors for heavy metal monitoring
(Taylor & Francis, 2026) Dorreh, Setareh; Kaya, Beril Sena; Aydemir, Zeynep; Al Faysal, Abdullah; Gölcü, Ayşegül
Heavy metal contamination remains a critical global environmental issue due to the persistence, bioaccumulation, and toxicity of metal ions such as Pb2+, Cd2+, Hg2+, and As³+. Although conventional analytical techniques provide high sensitivity and accuracy, they often rely on energy-intensive instrumentation, hazardous reagents, and generate considerable chemical waste, raising concerns regarding their environmental sustainability. In this context, molecularly imprinted polymer (MIP)-based electrochemical sensors have emerged as promising alternatives, offering high selectivity, operational simplicity, and compatibility with miniaturized and in situ analysis. This review critically examines the integration of Green Analytical Chemistry (GAC) principles into the design and fabrication of MIP-based electrochemical sensors for heavy metal monitoring. Particular attention is given to material selection, polymerization strategies, template removal approaches, and electrode modification techniques, with emphasis on their environmental implications. The applicability of quantitative greenness assessment tools, including the Analytical Eco-Scale, GAPI, AGREE, and AGREEMIP, is discussed in the context of sensor development workflows, highlighting both their strengths and current limitations in addressing fabrication stages, nanomaterial synthesis, and end-of-life considerations. By identifying methodological bottlenecks, particularly solvent-intensive template removal and limited reusability, this review outlines practical directions for advancing more sustainable sensor platforms. Overall, the work provides a critical framework for aligning analytical performance with environmental responsibility in next-generation MIP-based electrochemical sensing systems.
Fluoxetine attenuates hepatic ischemia–reperfusion injury through antioxidant, anti‑inflammatory and anti‑apoptotic mechanisms
(Springer Nature Link, 2026) Işık, Musab; Şahin, Gülderen; Kervancıoğlu, Gülnaz; Güner, İbrahim; Yaman, Muhittin Onur; Yelmen, Nermin
Hepatic ischemia–reperfusion injury is a major clinical problem associated with liver surgery, trauma, and transplantation, characterized by oxidative stress, inflammation, microcirculatory dysfunction, and apoptosis. Fluoxetine, a selective sero tonin reuptake inhibitor, has been reported to exert antioxidant, anti-inflammatory, and antiapoptotic effects. The present study aimed to investigate the potential protective effects of fluoxetine against experimental hepatic ischemia–reperfusion injury in rats. Male Wistar rats were randomly assigned to three groups: control, ischemia–reperfusion and fluoxetine-treated ischemia–reperfusion (n = 7 each). Hepatic ischemia–reperfusion injury was induced by infrarenal abdominal aortic cross clamping for 60 min followed by 120 min of reperfusion. Fluoxetine (20 mg/kg/day, intraperitoneal) was administered for three consecutive days prior to ischemia. Oxidative stress markers, antioxidant parameters, inflammatory and anti-inflamma tory cytokines, apoptotic markers, and specific tissue injury biomarkers were measured in liver homogenates using ELISA. Histopathological alterations were evaluated by light microscopy. Ischemia–reperfusion significantly increased oxidant markers, inflammatory cytokines, NF-κB activation, apoptotic indices, liver enzyme levels, and histological damage, while reducing antioxidant capacity. Fluoxetine treatment markedly restored antioxidant defenses, suppressed oxidative stress, inflammation, apoptosis, and microcirculatory injury, and significantly improved histopathological findings compared with the untreated ischemia–reperfusion group. Fluoxetine exerts a protective effect against hepatic ischemia–reperfusion injury through antioxidant, anti-inflammatory and antiapoptotic mechanisms, suggesting its potential as a therapeutic agent in conditions associated with hepatic ischemia–reperfusion.
Inhibition of c-jun n-terminal kinase attenuates diabetic testicular damage via endoplasmic reticulum stress reduction
(Universidad de la Frontera, 2026) Bayram, Şinasi; Ersoy, Onur; Deveci, Engin; Kızılay, Gülnur
The underlying causes of many diabetes-related complications are well known. However, the reasons for the complication related to male reproductive health remain unclear. Hyperglycemia disrupts the balance between oxidants and antioxidants, causing damage to cells, especially ER stress. ER stress triggered by the proteins accumulating in the ER lumen causes apoptosis by activating various pathways. c-Jun N-terminal kinase (JNK) is a key protein in systemic diseases like diabetes, and SP600125 is a widely used JNK inhibitor. This study focuses on whether JNK inhibition by SP600125 prevents diabetic testicular damage by reducing ER stress. In our study, animals were divided into three groups: Control group, the diabetes group, and the JNK inhibition group. Blood glucose level, body and testicular weights, and seminiferous tubule diameters were measured. Seminiferous tubules were evaluated by the Johnsen score in Hematoxyline and Eosin stained sections. Protein expressions of caspase 3, phospho (p)-JNK, caspase 12, and CHOP were evaluated. The Inhibitor group had significantly decreased active caspase-3, (p)-JNK, caspase-12, CHOP values, and blood glucose levels, increased body and testicular weights, seminiferous tubule diameter, and Johnsen score values compared to the diabetes group. JNK inhibition significantly ameliorated the histopathological damage in testicular tissue by preventing diabetes-induced ER stress and apoptosis.
