İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
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Güncel Gönderiler
The multifaceted effects of rosmarinic acid on breast cancer, regulating autophagy and increasing apoptosis
(Taylor & Francis, 2026) Erol Kutucu, Deniz; Erkısa Genel, Merve; Üvez, Ayça; Armutak, Elif İlkay; Yılmazer, Nadim; Demirci, Huri; Soluk Tekkesin, Merva; Ulukaya, Engin; Gürel Gürevin, Ebru
Cancer remains a complex and formidable disease that necessitates the development of diverse therapeutic strategies, including the investigation of natural products as complementary agents. Rosmarinic acid (RA), a phenolic compound, has demonstrated promising anticancer activity across various malignancies. Combining RA with conventional chemotherapeutic agents may represent a novel therapeutic approach. In the present study, the efficacy of these combinations was evaluated in vitro through the assessment of cell viability, apoptosis, autophagy, and proliferation, and in vivo by examining their effects on tumor growth in an Ehrlich Ascites Carcinoma (EAC) model. Among the tested combinations, RA and Paclitaxel (PTX) exhibited enhanced cytotoxicity and synergistic activity compared with individual treatments, whereas other combinations demonstrated limited efficacy. Morphological and moleculer analyses indicated induction of apoptosis, evidenced by increased expression of FAS, FADD, and cleaved caspases detected by Western blotting. Moreover, the RA+PTX (Rosmarinic acid+Paclitaxel) combination was associated with impaired autophagic flux, as reflected by elevated LC3 and p62 levels. Although the combined treatment reduced tumor volume in vivo, its antitumor efficacy was comparable to that of RA monotherapy. Collectively, these findings indicate that while the RA+PTX combination enhanced cytotoxicity activity against triple-negative breast cancer in vitro, its therapeutic advantage in vivo requires further investigation.
The effect of phosphoric acid on the development of neural tube defects in chick embryos
(International Scientific Information, Inc., 2026) Yücel, Murat; Çetin, Eyüp; Canbaz, Halime Tuba; Karip, Betül Zehra; Demir, Emine; Kocaoğlu, Sarper; Demir, Hüseyin
Introduction: Neural tube defects (NTDs) are among the most common congenital malformations and arise from disruption of early neurulation. Phosphoric acid is a widely used food additive; however, its potential effects on early neu ral tube development have not previously been evaluated in experimental neurulation models. This proof-of concept study aimed to investigate the embryotoxic and teratogenic effects of phosphoric acid on neural tube development in a chick-embryo model of neurulation, at a single tested concentration. Material/Methods: Fertilized pathogen-free chicken eggs (n=30) were randomly allocated into 2 groups. Control embryos (n=15) received no injection, whereas embryos in the experimental group (n=15) were injected beneath the embryon ic disc with 0.25 mM phosphoric acid, at Hamburger-Hamilton stage 9. Embryos were incubated for 72 hours, after which survival was recorded and neural tube development was evaluated macroscopically and histopath ologically. Statistical comparisons were performed using Fisher’s exact test. Results: All control embryos survived (15/15, 100%) and exhibited normal neural tube closure. In the phosphoric acid–treat ed group, survival was significantly reduced (10/15, 66.7%; P=0.0421). Among surviving treated embryos, 80% (8/10) demonstrated NTDs, including cranial and caudal closure abnormalities (P<0.001). Histopathological ex amination confirmed incomplete neural fold closure, irregular notochord morphology, and disrupted somite or ganization in affected embryos. Conclusions: Phosphoric acid exposure at the tested concentration and developmental stage markedly reduced embryo sur vival and induced a high incidence of neural tube closure defects in a chick-embryo model. These findings pro vide the first experimental proof-of-concept evidence that phosphoric acid can directly disrupt early neurula tion in a vertebrate neurulation model. However, vehicle-controlled replication, dose–response analyses, and exposure-bridging studies are required to distinguish teratogenic specificity from general embryotoxicity and to assess potential relevance to human embryogenesis.
Evaluation of the wound healing potential of volatile oil-loaded nanofibers
(Thieme, 2026) Kuruldak, Ebru; Durgun Kılıç, Meltem Ezgi; Olcay, F. N.; Aydın, M.; Aydilek, N.; Çelik, Y. S.; Gülsoy Toplan, G.
Volatile oils possess antimicrobial and anti-inflammatory activities beneficial for wound healing. In time, nanotechnological approaches like nanofibers have been developed to enhance volatile oils’ stability, bioavailability and controlled release [1] Our study aims to develop nanofibers loaded with a mixture of volatile oils to utilize in wound healing. A volatile oil mixture was prepared according to the book of Demirezer et al. [2]. Four volatile oils were used namely; pelargonium, chamomile, immortelle and tea tree oils were used as volatile oils to prepare the mixture. Polyvinylpyrrolidone (PVP) 360 was used for volatile oil-loaded nanofiber production. Formulations with polymer concentration of 15% (w/v) and 1 ml of volatile oil mixture were electrospun. The experimental setup varied in voltage, flow rate and collector distance. GC-MS analyzes of the mixture of oil and nanofibers were conducted. While the microbroth dilution method was applied to both volatile oil and the mixture oil, disc diffusion method used for the nanofiber parts. Strains were Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 [3] [4]. The results of antimicrobial tests are given in Table 1. The nanofibers collected in the collector formed a circle shape with a diameter of 5 cm in the production where a homogeneous fiber throw occurred. Only 4-terpinol, neryl acetate, geraniol and citronellol are found in GC-MS results of nanofibers. When all results were evaluated jointly, mixture volatile oil loaded nanofibers can be promising for wound healing activity however additional experiments should be designed by changing the parameters.
Effect of plaque-disclosing agents on biofilm removal: Single-center randomized trial in fourth-year dental students
(BioMed Central, 2026) Ballı Akgöl, Beyza; Bayram, Merve; Üstün, Nilüfer; Aksaka, Nurcan
Background To assess whether plaque-disclosing agents (PDA) improve self-performed mechanical plaque removal and gingival health compared with standard instruction alone in dental students. Design/Setting Single-center, parallel-group randomized controlled trial (1:1), Istanbul Medipol University (Oct 2023–Jan 2024). Participants 124 fourth-year dental students (age 20–30 years) randomized to test (n=62) or control (n=62). Interventions Both groups received standardized oral-hygiene instruction (Modified Bass; interdental cleaning guidance). The test group additionally received PDA before brushing as a visual aid; all outcomes were measured after triple rinsing. Primary outcome (prespecified) Between-group difference in change (Δ) in Plaque Index (PI) from baseline to week 1. Secondary outcomes ΔPI baseline→post-brushing and baseline→month 1; ΔGingival Index (GI) baseline→week 1 and baseline→month 1. Masking Outcome assessor blinded at the time of measurement (after rinsing). Results All 124 randomized participants completed follow-up and were analyzed. Both groups showed short-term improvement (immediate reduction in PI post-brushing; GI improvement at week 1). The primary outcome showed no significant between-group difference in ΔPI from baseline to week 1. Secondary analyses similarly found no significant between-group differences in ΔPI or ΔGI at month 1. Conclusions In a population with high baseline oral-hygiene proficiency, adding PDA did not confer a measurable advantage over standardized instruction alone on PI or GI change. PDA may be more informative in lay populations with lower oral-health literacy. Trial registration ClinicalTrials.gov, NCT06080672, registered on October 12, 2023.
The effect of covid 19 pandemic on childhood obesity in Turkey
(BioMed Central, 2026) Akalın, Hilal; Kılıç, Ayşe; Özçetin, Mustafa; Yıldız, İsmail; Kandemir, İbrahim; Varkal, Muhammet Ali; Yıldız, Melek
Aim: We aimed to investigate the effect of the coronavirus disease 2019 (COVID-19) pandemic on the prevalence of obesity in childhood, laboratory parameters associated with obesity and children's lifestyle changes. Patients and Method: We included exogenous children with obesity and overweight between the ages of 6 and 17 who applied to the General Pediatric Polyclinic of Istanbul Faculty of Medicine between 2018 and 2021. We allocated the participants to two groups: those who applied before and after April 2020 (when the first patient was diagnosed with COVID-19 in Türkiye) and compared anthropometric measurements, biochemical values, and imaging results. We also subjected a survey regarding the demographic characteristics of the cases, nutritional behaviors, and physical activities. Results: Sex distribution did not alter during the pandemic. However, the prevalence of exogenous obesity doubled, morbidly obese people rate increased, fasting insulin, insulin resistance, and low-density lipoprotein cholesterol (LDL-C) levels increased, and triglyceride levels decreased during the COVID-19 pandemic. There was no significant difference between the two periods regarding Hemoglobin A1c, alanine aminotransferase, aspartate aminotransferase, total cholesterol, high density lipoprotein levels, blood pressures, and hepatosteatosis. Consumption of pastries, packaged foods, and desserts increased during the pandemic. Also, children's physical activity decreased whereas screen time and sleep time increased. Adolescents' Food Habits Checklist scores did not alter significantly. Conclusion: There were significant increase in consuming unhealthy foods, becoming physically inactive, and screen time during pandemic with insulin resistance, obesity and increased LDL-C.
