İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
DSpace@İSTÜN, Üniversite mensupları tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Effect of plaque-disclosing agents on biofilm removal: Single-center randomized trial in fourth-year dental students
(BioMed Central, 2026) Ballı Akgöl, Beyza; Bayram, Merve; Üstün, Nilüfer; Aksaka, Nurcan
Background To assess whether plaque-disclosing agents (PDA) improve self-performed mechanical plaque removal and gingival health compared with standard instruction alone in dental students. Design/Setting Single-center, parallel-group randomized controlled trial (1:1), Istanbul Medipol University (Oct 2023–Jan 2024). Participants 124 fourth-year dental students (age 20–30 years) randomized to test (n=62) or control (n=62). Interventions Both groups received standardized oral-hygiene instruction (Modified Bass; interdental cleaning guidance). The test group additionally received PDA before brushing as a visual aid; all outcomes were measured after triple rinsing. Primary outcome (prespecified) Between-group difference in change (Δ) in Plaque Index (PI) from baseline to week 1. Secondary outcomes ΔPI baseline→post-brushing and baseline→month 1; ΔGingival Index (GI) baseline→week 1 and baseline→month 1. Masking Outcome assessor blinded at the time of measurement (after rinsing). Results All 124 randomized participants completed follow-up and were analyzed. Both groups showed short-term improvement (immediate reduction in PI post-brushing; GI improvement at week 1). The primary outcome showed no significant between-group difference in ΔPI from baseline to week 1. Secondary analyses similarly found no significant between-group differences in ΔPI or ΔGI at month 1. Conclusions In a population with high baseline oral-hygiene proficiency, adding PDA did not confer a measurable advantage over standardized instruction alone on PI or GI change. PDA may be more informative in lay populations with lower oral-health literacy. Trial registration ClinicalTrials.gov, NCT06080672, registered on October 12, 2023.
The effect of covid 19 pandemic on childhood obesity in Turkey
(BioMed Central, 2026) Akalın, Hilal; Kılıç, Ayşe; Özçetin, Mustafa; Yıldız, İsmail; Kandemir, İbrahim; Varkal, Muhammet Ali; Yıldız, Melek
Aim: We aimed to investigate the effect of the coronavirus disease 2019 (COVID-19) pandemic on the prevalence of obesity in childhood, laboratory parameters associated with obesity and children's lifestyle changes. Patients and Method: We included exogenous children with obesity and overweight between the ages of 6 and 17 who applied to the General Pediatric Polyclinic of Istanbul Faculty of Medicine between 2018 and 2021. We allocated the participants to two groups: those who applied before and after April 2020 (when the first patient was diagnosed with COVID-19 in Türkiye) and compared anthropometric measurements, biochemical values, and imaging results. We also subjected a survey regarding the demographic characteristics of the cases, nutritional behaviors, and physical activities. Results: Sex distribution did not alter during the pandemic. However, the prevalence of exogenous obesity doubled, morbidly obese people rate increased, fasting insulin, insulin resistance, and low-density lipoprotein cholesterol (LDL-C) levels increased, and triglyceride levels decreased during the COVID-19 pandemic. There was no significant difference between the two periods regarding Hemoglobin A1c, alanine aminotransferase, aspartate aminotransferase, total cholesterol, high density lipoprotein levels, blood pressures, and hepatosteatosis. Consumption of pastries, packaged foods, and desserts increased during the pandemic. Also, children's physical activity decreased whereas screen time and sleep time increased. Adolescents' Food Habits Checklist scores did not alter significantly. Conclusion: There were significant increase in consuming unhealthy foods, becoming physically inactive, and screen time during pandemic with insulin resistance, obesity and increased LDL-C.
Is LCP1 rs7324845gene variant related to obesity or NAFLD in adolescents
(Springer Nature, 2025) Yetim Şahin, Aylin; Sayın, Sevde Hasanoğlu; Kandemir, İbrahim; Oyacı, Yasemin; Kılıç, Ayşe; Pehlivan, Sacide
European Journal of Human Genetics
Determination of guaifenesin in spiked human breast milk: HPLC-UV method development, validation, and uncertainty evaluation
(Taras Shevchenko National University of Kyiv, 2025) Ceylan, Burhan; Önal, Cem; Önal, Armağan
A simple, sensitive, and reliable isocratic reversed-phase high-performance liquid chromatography (HPLC UV) method was developed, validated, and applied for the quantitative determination of guaifenesin in spiked human breast milk. Chromatographic separation was achieved on a C18 column (150 x 4.6 mm, 5 µm) using a mobile phase composed of methanol and water (50:50, v/v), where the aqueous phase was acidified with orthophosphoric acid (pH=3.2). The flow rate was 0.8 mL min-1, and detection was performed at 230 nm. The method exhibited excellent linearity over the concentration range of 5.0-30.0 ng mL-1 with a correlation coefficient of r2=0.9999. Liquid-liquid extraction (LLE) was used for sample preparation and resulted in a mean relative recovery of 98.82% with an absolute recovery of 99.52%, while effectively minimizing matrix interferences associated with breast milk. Method validation was performed in accordance with European Medicines Agency (EMA) bioanalytical guidelines, including assessments of selectivity, accuracy, precision, sensitivity, robustness, and stability. The method demonstrated strong reproducibility, did not require an internal standard, and provided a short analysis time suitable for routine application. This study presents the first simple, cost-effective, and sensitive HPLC-UV method for the determination of guaifenesin in human breast milk, offering a valuable analytical tool for evaluating drug safety during lactation.
The impact of conventional chemotherapy regimens and targeted drugs on ovarian function in breast cancer patients
(Springer Nature Link, 2026) Hasköylü, Şeyma; Şahin, Şevval Berfin; Altıntaş, Alara; Yıldız, Şule; Bildik, Gamze; Benlioğlu, Can; Turan, Volkan; Kim, Samuel; Öktem, Özgür
Breast cancer is the most common malignancy among women, affecting nearly 1.5 million individuals worldwide every year. While survival rates improve, many reproductive-age survivors confront significant long-term consequences, particu larly diminished ovarian reserve, infertility, and premature ovarian insufficiency due to the gonadotoxic effects of chemo therapy. Postponement of pregnancy for five years or more after treatment exacerbates the decline in fertility due to ongo ing ovarian aging and depletion of residual ovarian reserve. Women carrying BRCA1/2 mutations may already exhibit reduced ovarian reserve and are more vulnerable to gonadal damage, possibly due to impaired DNA repair mechanisms associated with these mutations. The contribution of other breast cancer susceptibility genes (e.g., ATM, CHEK2, PALB2, BARD1, RAD51C, RAD51D, and TP53) to chemotherapy-induced gonadotoxicity remains unclear. Although animal data shows depletion of primordial follicle pool and granulosa cells dysfunction, the ovarian effects of the poly(ADP-ribose) polymerase (PARP) inhibitors in women with and without BRCA mutation are not clear. Immune-check point inhibi tors (ICIs) causes immune-mediated destruction of the primordial follicle pool and reduction in ovarian reserve. Cyclin dependent kinase inhibitors appear to be less toxic than ICIs. In this narrative review of the current literature we aimed to provide a comprehensive overview of the molecular mechanisms underlying ovarian toxicity associated with conventional chemotherapy and targeted therapies in breast cancer treatment.
