İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
DSpace@İSTÜN, Üniversite mensupları tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
An update on fertility preservation from the asian society for fertility preservation
(Wiley, 2026) Turan, Volkan; Faizal Bin Ahmad, Mohd; Di, Wen; Gook, Debra; Kaul, Nalini; Kim, Seok Hyun; Ko, Jennifer; Chau Le, Thi Minh; Lee, Jung Ryeol; Öktem, Özgür
Fertility preservation (FP) is a rapidly expanding field in reproductive medicine with still limited data on outcomes. FP aims to protect the fertility of children, women, and men who face the potential risk of fertility loss for various medical conditions, including but not limited to cancer and its gonadotoxic treatment forms. Therefore, it is crucial to provide evidence-based recommendations to assist health professionals in discussing FP options. Our aim was to provide a guideline for multidiscipli nary medical staff in considering the availability of FP options and to help them decide whether to provide FP. The objective of any FP intervention is to minimize or eliminate primary disease burden and to ensure the maintenance or preserving reproductive health. Therefore, people who are at risk of losing fertility should be evaluated for and counseled about future reproductive risks. Embryo, oocyte, and ovarian tissue cryopreservation are the established FP options in adult females, with ovarian tissue cryopreservation the only option for prepubertal girls. A wide range of variables affect the choice of the FP strategy. These include age and ovarian reserve of women, the time available before the initiation of cancer treatment, puber tal status, and cancer type and stage. In males, sperm cryopreservation is a highly effective method in adolescent and adult males, while testicular tissue cryopreservation, which is experimental, is the only available option for prepubertal males. This review addressed the important clinical questions and provided answers for FP in females, males, and children according to the indications and availability of FP.
Complete healing after umbilical mesenchymal stem cell-derived exosome therapy for a refractory complex anal fistula
(Springer Nature Link, 2026) Arslan, Çiğdem; Erenler, İlknur; Karaöz, Erdal
Complex anal fistulas pose a persistent challenge due to high recurrence and the risk of continence impairment. While sphincter-sparing techniques exist, outcomes are variable in refractory disease. Mesenchymal stromal cell (MSC) therapies show promise but face logistical barriers, prompting interest in acellular exosome-based approaches. We report a 45-year-old woman with a complex anterior anal fistula refractory to multiple surgeries, including two endoanal advancement flaps, complicated by new-onset flatus incontinence. Following the second endoanal advancement flap, the patient's Cleveland Clinic Continence Score (CCIS) was 10, and the Quality of Life in Patients with Anal Fistula Questionnaire (QoLAF-Q) score was 51. A two-stage salvage strategy was undertaken: meticulous curettage and closure of the internal opening, followed by local administration of MSC-derived exosomes at baseline and three weeks. Significant wound reduction was observed, progressing to complete epithelialization within two weeks after the second exosome application. At six months after combined surgical intervention and exosome therapy, CCIS and QoLAF-Q scores improved to 0 and 28, respectively, indicating full restoration of continence and a marked improvement in quality of life. This case suggests that local exosome therapy, used as an adjunct to careful surgical preparation, may support healing and functional recovery in refractory complex anal fistulas. Controlled studies with standardized protocols are warranted to define efficacy, dosing, and durability.
Phenol for pilonidal sinus disease: A nationwide survey of practice patterns and safety gaps among surgeons in Türkiye
(BioMed Central, 2026) Ergüder, Ender; Yahya, Duha; Turan, Ersin; Şimşek, Gürcan; Bulut, Sezer; Arslan, Çiğdem
Background Phenol is widely used as a minimally invasive treatment for pilonidal sinus disease (PSD); however, its clinical application is characterized by substantial heterogeneity in indications, technique, dosing, and safety practices. Despite its routine use in outpatient settings, standardized safety frameworks addressing occupational exposure, environmental controls, and dose documentation are lacking. These gaps may have important implications for both patient outcomes and workplace safety. This nationwide survey aimed to characterize real-world phenol practice patterns among surgeons in Türkiye, with a particular focus on safety infrastructure, exposure control measures, and clinical decision-making. Methods A nationwide, internet-based, cross-sectional survey of surgeons managing PSD in Türkiye (July 8– August 15, 2025), aligned with CHERRIES. The 38-item instrument captured demographics; workload/experience; environmental controls (ventilation), institutional exposure-prevention policies, and personal protective equipment (PPE); patient-side protection strategies; phenol procurement, formulation/concentration, and dosing; treatment planning; and outcomes/perceptions. Descriptive statistics summarized item-level responses. Results A total of 132 surgeons provided responses. 81.1% evaluated more than 30 PSD patients annually, and 48.5% conducted more than 30 phenol procedures per year. Surgeons frequently procured phenol from external pharmacies (36.4%); crystalline phenol predominated (77.3%); 50.8% did not know the concentration (most reported 99.9%); and only 7.6% measured dose per procedure. The majority of surgeons performed 2 to 3 sessions (69.0%) at intervals of 2 to 3 weeks (38.6%). Key safety gaps were identified: only 34.8% reported dedicated ventilation, 53.0% reported none, and 62.1% had no institutional exposure-prevention policy. Routine mask use was reported by 53.8% (primarily surgical masks); additional PPE beyond mask and gloves was used by 22.0% (gowns 51.7%, eye protection 48.3% among those reporting). Patient-side skin protection was common (81.3%). Adverse events in patients were recorded at a rate of 20.5%, with skin burns being the most prevalent, while operator adverse effects occurred at a rate of 5.3%. Despite these gaps, perceived safety was relatively high (patients 83.3%, operators 68.9%), and 89.4% would personally choose phenol if affected. Conclusions Findings support a safety-first national framework specifying permissible formulations/concentrations, mandating dose documentation and skin-protection protocols, and implementing a core safety bundle (effectiveventilation, written exposure-control policies/checklists, and PPE including eye protection). Prospective exposure monitoring with standardized outcomes is warranted.
Synergistic cytotoxicity of bentonite–zeolite 4A nanocomposite in human melanoma cells
(Taylor & Francis, 2026) Duman, Nilay; Evcin, Atilla; Çelik, Sefa; Oraloğlu, Göktürk; Caner, Ayşe
The cytotoxic effects of bentonite and zeolite 4A nanoparticles (NPs), as well as their nanocomposite (NC), were investigated in human melanoma (G361) cells. Although both materials have demonstrated anticancer potential, their effects on melanoma remain insufficiently explored. This study aimed to evaluate the cellular responses induced by bentonite and zeolite 4A NPs, individually and in combination, in G361 cells. Methods: Physicochemical characterization was performed using X-ray diffraction (XRD), X-ray fluorescence (XRF), and scanning electron microscopy (SEM). IC₅₀ values were determined for bentonite NPs, zeolite 4A NPs, and the NC. Cell viability was assessed using the MTT assay, while apoptosis was evaluated through caspase-3 activity measured by ELISA. Results: All treatments exhibited dose-dependent cytotoxicity in melanoma cells. Notably, the NC demonstrated a strong synergistic interaction, enhancing cytotoxic effects and enabling reduced effective concentrations. Despite the pronounced cytotoxicity observed in the NC group, no significant increase in caspase-3 activity was detected compared to control. These findings suggest the involvement of caspase-independent cell death mechanisms, particularly necrotic or non-classical apoptotic processes. Conclusion: The bentonite/zeolite 4A nanocomposite exerts synergistic cytotoxic effects in melanoma cells. While the results highlight its therapeutic potential, further mechanistic studies are required to clarify the underlying pathways prior to clinical translation.
Aesthetic preformed paediatric crowns in primary incisors: A bibliometric study and critical analysis
(Springer Nature Link, 2026) Taşdemir, Tülin; Erbaş Ünverdi, Gizem; Çehreli, Zafer Cavit
Purpose The aim of this bibliometric study was to assess clinical research regarding preformed crowns used for the treat ment of primary anterior teeth. Methods A search was conducted in the Web of Science Core Collection (WoSCC) for publications on preformed crowns for primary anterior teeth, and was completed in a single day (August 8, 2025), identifying 379 articles. After filtering for original research and case report/series published in English, 40 relevant articles were selected for review. These publications were evaluated based on annual publication trends, study designs, journals, citation counts, authors, and keywords, using the ‘biblioshiny’ program in RStudio. Additionally, the review included critical analysis of articles with full texts to collect detailed information on study design, sample size, and clinical outcomes. Results The review of 40 articles over three decades showed a 4.57% annual increase in publication number on preformed crowns for primary anterior teeth, especially since 2012, with USA authors leading. The data reveal temporal trends in preveneered preformed metal crowns for anterior primary teeth, strip crown success, zirconia crowns (gingival health, qual ity of life and parental satisfaction comparisons), and preformed crowns for primary anterior teeth after pulpal treatment. Amongst them, the journal “Pediatric Dentistry” had published the most papers. Recent studies highlighted zirconia crowns’ high retention and gingival health benefits, whilst strip crowns showed lower durability. Conclusion For anterior primary teeth, that zirconia crowns offered high durability, aesthetics, and parental satisfaction. The heterogeneity of study designs underscores the necessity for more comprehensive research to inform paediatric dentistry practices.
