İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
DSpace@İSTÜN, Üniversite mensupları tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
A green electrochemical sensor based on molecular imprinting for etoposide detection in environmental matrices
(Elsevier, 2026) Al Faysal, Abdullah; Kaya, Beril Sena; Dorreh, Setareh; Erdoğan, Taner; Gölcü, Ayşegül
A semisynthetic form of podophyllotoxin, etoposide (ETO), is frequently used to manage multiple types of cancer, including lung, testicular, bladder, prostate, and gastric malignancies. Having been utilized in clinical settings for over twenty years, it is one of the most frequently prescribed anticancer agents globally. The primary cytotoxic mechanism of ETO involves the inhibition of topoisomerase II. In the present work, an innovative electrochemical detection platform utilizing MIP was successfully established to enable both highly selective and exceptionally sensitive determination of ETO in pharmaceutical injection forms and environmental specimens. An ETO-specific MIP sensor was fabricated through a photopolymerization process and immobilized onto a GCE, where AMPS served as the active monomer, and EGDMA functioned as the cross-linking agent. This study marks the first instance of a MIP-based electrochemical sensor designed explicitly for ETO identification. The AMPS ETO@MIP/GCE sensor was subjected to electrochemical and morphological assessments through FTIR, SEM, CV, and EIS. An indirect measurement approach was employed using a 5.0 mM potassium ferricyanide/ferro cyanide system to ascertain the analytical detection range from 1.0 to 10.0 pM. The sensor demonstrated excellent sensitivity, reproducibility, and selectivity, enabling effective discrimination of ETO from structurally similar compounds while retaining reliable performance in complex matrices such as soil and tap water. Results from validation experiments in pharmaceutical matrices indicated superior recovery, supporting the sensor's practical effectiveness and stability. To further investigate the experimental results and better understand the nature of template–monomer interactions, a series of DFT calculations was performed. Binding energies were evaluated for ETO–AMPS complexes across varying template-to-monomer ratios.
Group-based CBTgrief for complicated grief: Cultural implementation of a manualized protocol in Türkiye
(Wiley, 2026) Savaş, Esra
Group-based CBTgrief for complicated grief: Cultural implementation of a manualized protocol in Türkiye
Anthropometric analysis of nasal widening following bimaxillary orthognathic surgery
(Wolters Kluwer, 2026) Öztürk Muhtar, Merve; Yey Özkeskin, Sabahat Zeynep; Küçükçakır, Osman; Keskin Yalçın, Başak; Cansız, Erol
Orthognathic surgery aims to improve jaw function and aesthetics, but these procedures can also cause undesired changes in the nasal region, a crucial component of facial ap pearance. An alar cinch suture has been proposed to mitigate such nasal widening. This study aimed to evaluate changes in the interalar distance (distance between the right and left alar curvature points) and alar base distance (distance between the right and left alar base points) in patients who underwent bi maxillary orthognathic surgery with an alar cinch suture. This retrospective study included patients who underwent bimaxil lary orthognathic surgery at the Istanbul University Faculty of Dentistry Department of Oral and Maxillofacial Surgery from 2012 to 2018. Interalar and alar base distances were measured on standardized frontal photographs taken preoperatively and at 6 months postoperatively. Statistical evaluation was per formed using nonparametric methods, including the Wilcoxon signed-rank test applied for preoperative and postoperative comparisons and Spearman rho used to assess correlations, with the significance level set at P < 0.05. The interalar distance increased significantly from preoperative to postoperative measurements (median value: 8.04%; mean change: 7.33 ± 5.38%; P = 0.001). Similarly, the alar base distance showed a significant increase from preoperative to post operative measurements (median value: 11.90%, mean change: 12.49 ± 9.57%; P = 0.001). No statistically significant correla tion was found between the percentage changes in interalar and alar base distances (Spearman ρ = 0.201, P = 0.359). A tendency toward nasal soft tissue widening was noted after Le Fort I advancement, despite alar cinch suture application.
Predictors of re-biopsy in percutaneous musculoskeletal tumor biopsies: A single-center retrospective cohort study
(SAGE Publications, 2026) Yücesan, Ali; Arıkan, Yavuz; Doğukan, Fatih Mert; Mutlu, İlhan Nahit; Toy, Serdar; Atan, Tamer; Özer, Devrim
Purpose: This study aimed to evaluate the diagnostic success of percutaneous biopsies performed for suspected mus culoskeletal tumors and to identify clinical, anatomical, and procedural factors associated with the need for repeat biopsy (re-biopsy). The secondary objective was to assess the impact of multidisciplinary team (MDT) evaluation on reducing unnecessary re-biopsy procedures. Methods: A total of 565 patients (331 bone, 234 soft tissue lesions) who underwent percutaneous biopsy for suspected musculoskeletal tumors between 2020 and 2024 were retrospectively analyzed. Diagnostic success was defined as a biopsy yielding a definitive histopathological diagnosis sufficient to guide treatment planning without additional tissue sampling. Predictive factors including lesion size, depth, location, and performing specialty were evaluated using univariate and multivariate logistic regression analyses. Results: The initial diagnostic yield was 65.5%. Multivariate analysis revealed that lesion width <2 cm (OR = 2.01; 95% CI, 1.19–3.39; p = 0.009) and biopsy performed by an interventional radiologist (compared to an orthopedic surgeon) (OR = 5.25; 95% CI, 2.83–9.74; p < 0.001) were independent predictors of re-biopsy recommendation. Among 72 patients recommended for re-biopsy, MDT evaluation averted the need for a second procedure in 38 cases (52.8%). Conclusion: Smaller lesion size (<2 cm) and the medical specialty performing the biopsy were independent predictors of re-biopsy recommendation, with the latter likely reflecting systematic differences in case complexity and procedural environment rather than operator skill. Structured MDT evaluation significantly reduced unnecessary repeat procedures.
Thermoresponsive sol–gel system incorporating oleuropein-rich olive leaf extract for enhanced wound healing and antibiofilm activity
(MDPI Publishing, 2026) Alparslan, Levent; Özdemir, Samet; Karacan, Burak; Torkay, Gülşah; Bal Öztürk, Ayça; Tutar, Ömer Faruk; Özcan Bülbül, Ece; Sardaş, Semra; Kala, Zübeyde Merve; Özalp, Yıldız
Oleuropein, the principal secoiridoid phenolic compound of olive leaves (Olea europaea L.), is recognized for its broad-spectrum antimicrobial, antibiofilm, antioxidant, and tissue regenerative properties. However, its effective local therapeutic application remains chal lenging due to rapid clearance from the site of administration and limited residence time. In this study, an oleuropein-rich aqueous olive leaf extract was incorporated into a ther moresponsive sol–gel delivery system designed for localized application. The formulation was engineered to remain in a low-viscosity sol state at room temperature and to undergo a temperature-triggered sol-to-gel transition near physiological temperature (~33 ◦C), en abling in situ gel formation. Oleuropein content was quantified using a validated HPLC method, and the formulation was characterized with respect to physicochemical parame ters, thermoreversible gelation behavior, particle size distribution, mechanical properties, and spreadability. Biological performance was evaluated through in vitro cytocompati bility (MTT assay), fibroblast migration (scratch assay), and collagen deposition (Sirius Red staining) in L929 fibroblasts, as well as antibiofilm activity against representative Gram-positive and Gram-negative bacterial strains. The developed sol–gel system demon strated stable physicochemical characteristics, rapid and reversible thermogelation, suitable mechanical and spreading properties, concentration-dependent inhibition of biofilm for mation, and acceptable cytocompatibility within the tested concentration range. Notably, the formulation supported fibroblast viability and collagen-associated responses at opti mized concentrations. Overall, the results indicate that the proposed thermoresponsive sol–gel formulation represents a promising strategy for the localized delivery of oleuropein rich olive leaf extract, combining physicochemical stability with dual wound-healing and antibiofilm functionality.
