İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
DSpace@İSTÜN, Üniversite mensupları tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Selective effect of atmospheric non-thermal plasma on resin cement shear bond strength to glass ceramics
(BioMed Central, 2026) Mustafaoğlu, Emine; Ercan, Utku Kürşat; Özdemir, Gizem Dilara; Bağış, Bora
Statement of problem The effectiveness of non-thermal plasma (NTP) in improving the bond strength of different resin cements to different glass ceramics under various surface treatment protocols remains unclear. Moreover, the influence of specific plasma devices and the material-dependent response of ceramic–resin cement systems to NTP have not been sufficiently investigated. Purpose The purpose of this in vitro study was to evaluate the effect of NTP on the shear bond strength of different resin cements to different glass ceramics by comparatively assessing multiple surface treatment protocols. Materials and methods A total of 240 specimens of each glass ceramic group (IPS e.max CAD (E), Vitamark II (V)) were divided into 24 groups according to the combination of 6 surface treatments ((control), hydrofluoric acid (HF)+ceramic primer (Pr), Pr, HF+plasma (Pl) + Pr, Pl + Pr, and Pl) and two different resin cements (Single Bond+RelyX Ultimate (R) and ZenitCem (Z)). NTP was applied with an AC microsecond pulse power supply (5 kHz, 20 kV, 90 s). Specimens were cemented to composite cylinders. After storage in an oven, SBS was measured. The results were analyzed using three-way ANOVA, post-hoc tests, and targeted pairwise comparisons, with the significance level set at α = 0.05. The effectiveness of NTP is material- and cement-dependent and should be considered as a selective surface modification strategy rather than a universal alternative to conventional surface treatments. Results The V-Pl-Z group showed significantly higher SBS values than the V-Z group, and these values were within the clinically acceptable range (p < 0.05). In the V groups treated with HF + Pr and HF + Pl+Pr, R demonstrated higher bond strength values than Z. HF surface treatment increased the SBS values for all groups (p < 0.05). Conclusions The effectiveness of NTP is material- and cement-dependent and should be considered as a selective surface modification strategy rather than a universal alternative to conventional surface treatments Clinical significance This study demonstrates that atmospheric non-thermal plasma does not universally enhance ceramic–resin cement bonding. Its effectiveness depends on the ceramic substrate, resin cement type, and surface treatment protocol. Clinicians should therefore apply plasma selectively.
Integrating green chemistry principles into the fabrication of MIP electrochemical sensors for heavy metal monitoring
(Taylor & Francis, 2026) Dorreh, Setareh; Kaya, Beril Sena; Aydemir, Zeynep; Al Faysal, Abdullah; Gölcü, Ayşegül
Heavy metal contamination remains a critical global environmental issue due to the persistence, bioaccumulation, and toxicity of metal ions such as Pb2+, Cd2+, Hg2+, and As³+. Although conventional analytical techniques provide high sensitivity and accuracy, they often rely on energy-intensive instrumentation, hazardous reagents, and generate considerable chemical waste, raising concerns regarding their environmental sustainability. In this context, molecularly imprinted polymer (MIP)-based electrochemical sensors have emerged as promising alternatives, offering high selectivity, operational simplicity, and compatibility with miniaturized and in situ analysis. This review critically examines the integration of Green Analytical Chemistry (GAC) principles into the design and fabrication of MIP-based electrochemical sensors for heavy metal monitoring. Particular attention is given to material selection, polymerization strategies, template removal approaches, and electrode modification techniques, with emphasis on their environmental implications. The applicability of quantitative greenness assessment tools, including the Analytical Eco-Scale, GAPI, AGREE, and AGREEMIP, is discussed in the context of sensor development workflows, highlighting both their strengths and current limitations in addressing fabrication stages, nanomaterial synthesis, and end-of-life considerations. By identifying methodological bottlenecks, particularly solvent-intensive template removal and limited reusability, this review outlines practical directions for advancing more sustainable sensor platforms. Overall, the work provides a critical framework for aligning analytical performance with environmental responsibility in next-generation MIP-based electrochemical sensing systems.
Fluoxetine attenuates hepatic ischemia–reperfusion injury through antioxidant, anti‑inflammatory and anti‑apoptotic mechanisms
(Springer Nature Link, 2026) Işık, Musab; Şahin, Gülderen; Kervancıoğlu, Gülnaz; Güner, İbrahim; Yaman, Muhittin Onur; Yelmen, Nermin
Hepatic ischemia–reperfusion injury is a major clinical problem associated with liver surgery, trauma, and transplantation, characterized by oxidative stress, inflammation, microcirculatory dysfunction, and apoptosis. Fluoxetine, a selective sero tonin reuptake inhibitor, has been reported to exert antioxidant, anti-inflammatory, and antiapoptotic effects. The present study aimed to investigate the potential protective effects of fluoxetine against experimental hepatic ischemia–reperfusion injury in rats. Male Wistar rats were randomly assigned to three groups: control, ischemia–reperfusion and fluoxetine-treated ischemia–reperfusion (n = 7 each). Hepatic ischemia–reperfusion injury was induced by infrarenal abdominal aortic cross clamping for 60 min followed by 120 min of reperfusion. Fluoxetine (20 mg/kg/day, intraperitoneal) was administered for three consecutive days prior to ischemia. Oxidative stress markers, antioxidant parameters, inflammatory and anti-inflamma tory cytokines, apoptotic markers, and specific tissue injury biomarkers were measured in liver homogenates using ELISA. Histopathological alterations were evaluated by light microscopy. Ischemia–reperfusion significantly increased oxidant markers, inflammatory cytokines, NF-κB activation, apoptotic indices, liver enzyme levels, and histological damage, while reducing antioxidant capacity. Fluoxetine treatment markedly restored antioxidant defenses, suppressed oxidative stress, inflammation, apoptosis, and microcirculatory injury, and significantly improved histopathological findings compared with the untreated ischemia–reperfusion group. Fluoxetine exerts a protective effect against hepatic ischemia–reperfusion injury through antioxidant, anti-inflammatory and antiapoptotic mechanisms, suggesting its potential as a therapeutic agent in conditions associated with hepatic ischemia–reperfusion.
Inhibition of c-jun n-terminal kinase attenuates diabetic testicular damage via endoplasmic reticulum stress reduction
(Universidad de la Frontera, 2026) Bayram, Şinasi; Ersoy, Onur; Deveci, Engin; Kızılay, Gülnur
The underlying causes of many diabetes-related complications are well known. However, the reasons for the complication related to male reproductive health remain unclear. Hyperglycemia disrupts the balance between oxidants and antioxidants, causing damage to cells, especially ER stress. ER stress triggered by the proteins accumulating in the ER lumen causes apoptosis by activating various pathways. c-Jun N-terminal kinase (JNK) is a key protein in systemic diseases like diabetes, and SP600125 is a widely used JNK inhibitor. This study focuses on whether JNK inhibition by SP600125 prevents diabetic testicular damage by reducing ER stress. In our study, animals were divided into three groups: Control group, the diabetes group, and the JNK inhibition group. Blood glucose level, body and testicular weights, and seminiferous tubule diameters were measured. Seminiferous tubules were evaluated by the Johnsen score in Hematoxyline and Eosin stained sections. Protein expressions of caspase 3, phospho (p)-JNK, caspase 12, and CHOP were evaluated. The Inhibitor group had significantly decreased active caspase-3, (p)-JNK, caspase-12, CHOP values, and blood glucose levels, increased body and testicular weights, seminiferous tubule diameter, and Johnsen score values compared to the diabetes group. JNK inhibition significantly ameliorated the histopathological damage in testicular tissue by preventing diabetes-induced ER stress and apoptosis.
Parental awareness, knowledge, and hesitancy toward HPV vaccination for adolescents: A cross-sectional study
(Sociedad Argentina de Pediatria, 2026) Güven, Deniz; Köle, Mehmet Tolga; Örün, Emel; Çaylan, Nilgün; Küçük, Elif; Kandemir, İbrahim; Öztürk, Seval; Kondolot, Meda
Background. Human papillomavirus (HPV) is a prevalent sexually transmitted infection and major cause of cervical and anogenital cancers. This study aimed to assess parental knowledge, vaccine hesitancy, and health beliefs concerning HPV infection and vaccination. Methods. This cross-sectional study was conducted using convenience sampling among parents of adolescents aged 9–18 attending outpatient clinics at a tertiary hospital. The study collected sociodemographic characteristics and used the Health Belief Model Scale for HPV Infection and Vaccination to assess health beliefs, in order to identify factors associated with parental perceptions and vaccination attitudes. Results. A total of 320 parents participated in the study; 82.8% were mothers. Self-reported HPV infection was low (1.9%), and only 3.4% of parents and 2.8% of their children had been vaccinated. Awareness about HPV infection was 75%, and that of the HPV vaccine was 67.2%. However, only 30% of parents were willing to vaccinate their children, while 47.5% were uncertain. The most commonly reported barriers were lack of awareness (33.9%) and concerns about side effects (26.4%). Higher parental education, family income, and prior HPV knowledge were positively associated with both awareness and willingness to vaccinate children, whereas having multiple adolescents was associated with increased vaccine hesitancy. Conclusion. Parental awareness of HPV infection and vaccination was 75% and 67.2%, respectively, while vaccination rates among adolescents were very low. Higher parental education, income, and prior HPV knowledge were associated with favorable perceptions of vaccination. Addressing knowledge gaps and reducing perceived barriers through educational interventions may help increase HPV vaccination uptake among adolescents.
