İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
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Güncel Gönderiler
The impact of culture conditions on mitochondrial dynamics in different cell types
(Bursa Uludağ Üniversitesi, 2025) Köm, Doğa Buse; Çataltepe, Sude Naz; Budak, Gülden; Dal Yöntem, Fulya
Aim: Mitochondria continuously undergo fusion and fission processes to maintain their structural integrity and function and mitochondrial dynamics can vary between cells representing various pathologies. Culture conditions such as seeding density, passage number, and incubation time, which are commonly manipulated in cell culture studies, may influence mitochondrial morphology. The observation that the same cell line can exhibit both fusion and fission dominant phenotypes suggests that culture conditions play a role in shaping mitochondrial dynamics. This study aims to investigate the effects of in vitro culture conditions on mitochondrial dynamics. Material and Method: A549 (human lung cancer cells), BEAS-2B (human bronchial epithelial cells), MCF7 (human breast cancer cells), A375 (human malignant melanoma cells), and RPE1 (human retinal pigment epithelial cells) were incubated at early (passage 10–12) and late (passage 20–22) passages under varying seeding densities and durations (24, 48, and 72 hours). Mitochondria were stained with MitoTracker Green, and morphological analysis was performed using fluorescence microscopy. Mitochondrial fragmentation was assessed using ImageJ Fiji software, and statistical analyses were conducted using GraphPad software. Results: In all cell lines, the culture condition of 100,000 cells for 24 hours was used as the control group, and mitochondrial fragmentation was observed to vary depending on cell type, incubation duration, and cell density. In A549 cells, fragmentation at 48 hours with 100,000 cells was found to be five times higher compared to 72 hours with 80,000 cells. In MCF-7 cells, fragmentation at 72 hours with 80,000 cells was approximately 1.6 times higher than at 48 hours with 170,000 cells. In BEAS-2B and RPE1 cells, no significant changes in mitochondrial dynamics were observed in response to incubation time or cell density. Conclusion: Prolonged culture duration increases mitochondrial fusion in A549 cells, whereas both incubation time and cell density notably affect mitochondrial fission in MCF7 cells. In contrast, mitochondrial dynamics in BEAS-2B and RPE1 cells are not significantly influenced by incubation time or cell density. These findings suggest that cell type–specific culture conditions can have distinct impacts on mitochondrial morphology.
Branching patterns and variations of the anterior choroidal artery: A detailed cadaveric morphometric analysis
(Springer Nature Link, 2025) Kaçur, İdil; Nteli Chatzioglou, Gkionoul; Nas, Emine; Gayretli, Özcan
The aim of our study was to investigate the morphometric and morphological characteristics of the anterior choroidal artery (AChA) and its branches. Also, the branching patterns and cortical termination sites of the AChA were examined by detailed morphometric analysis. For this purpose, 62 fresh cadavers (124 hemispheres) were included in the study. AChA diameter, length, and distances of branches to the AChA origin were measured using ImageJ software. The distribution of the branches according to arterial origin and their distances to the target regions was quantitatively defined. Although a total of 594 branches originating from AchA were observed, only 587 of these branches reached or terminated in the regions observed. Accordingly, 220/587 were found to go to the optic tract, 214/587 to the cerebral peduncle, 130/587 to the uncus, and 23/587 to the anterior perforated substance. Our study provides novel morphometric ratios that map the branching architecture of the AChA, confirming that branches originate predominantly from its midpoint (overall ratio:0.45). We found that 73.07% of AChA branches supply the optic tract and cerebral peduncle, highlighting their central role, while a minimal proportion (3.87%) serve the anterior perforated substance. These data elucidate the topo graphical relationships that are crucial for understanding pathologies like Moyamoya disease and proximal aneurysms. The established ratios offer invaluable benchmarks for anatomical navigation, potentially reducing risks in neurosurgical procedures involving the AChA territory.
Sustainable human resource management: A proposal of comprehensive model
(Emerald Insight, 2025) Yalırsu, Hazal Yaren; Tüzüner, Vala Lale
The significance of human resource management (HRM), which positively impacts the organization’s strategical development and sustainability, has progressively increased and has undergone a transformation throughout time. It is important to highlight that whereas literature emphasizes the role and goals of human resources for fostering sustainability, there is no widely accepted approach. Aside from considering the organization’s competitive advantages, it also takes into consideration social and environmental aspects while considering all its stakeholders. In doing so, it must also ensure its financial sustainability. Despite the existence of several fundamental methods, the absence of a comprehensive approach that encompasses every component leads to the ongoing discussions in the literature on sustainable HRM. Furthermore, the sustainable human resources literature lacks to address the impact of institutional pressures, resulting in an insufficient approach. In this study, many core approaches and alternative perspectives in literature have been evaluated from a comprehensive perspective, leading to the development of a recommended approach which institutional pressures were added.
Chia seeds-based gel electrolyte for sustainable and stable zinc ion batteries
(ESC-IS, 2025) Uzunoğlu, Gülşah Yaman
Aqueous zinc batteries represent a promising option for energy storage and conversion technologies in the "post-lithium" era, owing to their elevated energy density, enhanced safety, and affordability. Rechargeable aqueous zinc ion batteries (RZBs) are recognized as promising alternatives to lithium-ion batteries due to their cost-effectiveness, safe-and-sustainable by-design, and scalability. Nonetheless, their performance is hindered by inadequate cyclability, which is attributed to dendrite formation and the hydrogen evolution reaction (HER) occurring at the zinc anode [1].
Labia majora lifting technique with polydioxanone threads
(Oxford University Press, 2025) Ağlamış, Savaş Özgür; Akkaya, Selver Kübra; Şahin, Elif; Şahin, Hanifi
Background: Polydioxanone (PDO) thread is a synthetic absorbable surgical suture used for rejuvenation and lifting. Aim: The aim of this study is to use PDO threads for rejuvenation and enlargement effect in patients with labium majus hypotrophy. Methods: Twenty-one patients with labia majora hypotrophy were included in the study. Conventionally, surgery, fat filling or hyaluronic acid filling is used for labia majora rejuvenation. In this study, a different technique, the PDO thread suspension technique, was applied. For PDO thread, Hyundae Meditech Co.Ltd’s Secret Line Up product containing 50 mm screw thread with 30 G-38 mm needle tip was used. It was planned to use 10 PDO threads for right and left labia majora. After a total of 20 needles were inserted, the needles were removed one by one and the PDO threads remained in the subcutaneous superficial layer and the procedure was terminated 5 min later. Preoperative and postoperative the Female Genital Self-Image Scale (FGSIS) scores of the patients were compared. Outcomes: The overall FGSIS total score demonstrated a significant increase following the intervention. Results: The FGSIS total mean score in the preoperative period was increased in the postoperative period. This difference was statistically significant. Moreover, the mean score calculated for each parameter of FGSIS in the preoperative period increased significantly in the postoperative period. Clinical Implications: These findings indicate a favorable safety profile for the use of PDO threads in this clinical context. Strengths and Limitations: The strength of the study is to introduce a minimally invasive and effective method for labia majora lifting, on the other hand, the small number of patients in the study, limitation of the study. Conclusion: We would like to point out that in this study, we evaluated labium majus rejuvenation from the same perspective, based on the shaping and enlargement of genital appearance and its positive effect on self-confidence and increase in sexual functions. Unlike many labium majus rejuvenation procedures, this less invasive procedure has achieved similar results. In this context, it is a preferable alternative to surgery.
