Yazar "Dikmen, Tayfun" seçeneğine göre listele

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    Characterization of bovine bone marrow derived mesenchymal stem cells and immunostaining of differentiated neurospheres
    (Hellenic Veterinary Medical Society, 2023) Dikmen, Tayfun; Özden Akkaya, Özlem; Nawaz, Shah; Erdoğan, Metin
    Stem cell differentiation has been a key element for the therapeutical applications for various disorders. The novel anti-inflammatory and angiogenic findings and potential applications for both autologous and allogenic studies have emphasized about the usage of mesenchymal stem cells in the clinical trials. The neurosphere differentia-tion studies and their role for peripheral nerve injuries have gained momentum recently. Following study emphasized on the isolation of stem cells from bovine bone marrow and characterization of their mesenchymal potential by PCR followed by their differentiation into mesodermal lineages and into neurospheres under specific induction media. The generated neurospheres were further investigated for their potential expressions of neuroprogenitor markers Sox2, Nestin and neurofilament protein β-III Tubulin by immunofluorescence staining. The results of following study high-light the unique potential of bone marrow derived mesenchymal stem cells for translational research and regenerative therapy in nervous tissue injuries.
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    Characterizing the amniotic fluid-derived stem cells and optimizing the passage number for targeted applications
    (Faculty Of Veterinary Science, University Of Agriculture, 2024) Dikmen, Tayfun; Erdoǧan, Metin; Altunbaş, Korhan
    Mesenchymal stem cells are considered potent sources that can be used for tissue regeneration and treatment of various diseases and syndromes. Among the mesenchymal stem cells, amniotic fluid-derived stem cells come forward as they possess some pluripotent properties and there are no serious ethical concerns upon their derivation. Therefore, gaining a better understanding of the nature of amniotic fluid stem cells is important. Although it is known that stem cells show slightly different characteristics between passages, scientists often consider only cell numbers and the proliferation pattern of the cells when deciding the passage to use in their studies. In this study, it was aimed to characterize rat amniotic fluid-derived stem cells for their mesenchymal and pluripotent features and make inter-passage comparisons by real-time qPCR to reveal their distinctions between different passages, and eventually help decide the appropriate passage numbers to be used in future research. The outcomes of the study showed that using rat amniotic fluid-derived stem cells at P5 could be beneficial for mesodermal differentiation studies and using them in earlier passages may be more favorable for the studies requiring better ectodermal differentiation properties.
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    Histological staining technics for identifying various structures for histological assessment of bone and cartilage healing
    (ICBH, 2023) Dikmen, Tayfun
    Bone and cartilage regeneration are challenging topics which the scientists are constantly looking for new approaches to eliminate current limitations of the traditional therapeutic methods which are being used. Cellular therapy and use of biomaterials for that purpose is providing encouraging results, especially in cases like osteoarthritis and bone healing. In such studies, the histological assessment of the healing of corresponding tissues are vital to determine to see if the developed method is indeed resulted with positive outcomes or not. Each tissue has some different distinctive structures that must be investigated during the healing process. There are several histological staining technics being used for the evaluation of the healing of bone and cartilage tissues by identifying the structures of interest. Among them, hematoxylin eosin staining comes forward as the most applied technic. However, it is not suitable to identify each and every important histological structure, and some specific staining technics are necessary to identify such structures like new bone formation areas, mineralized bone areas, glycosaminoglycan aggregates, collagen fiber formation, etc; regarding the aim of the research. In this study, bone and articular cartilage samples were taken and stained with Alcian blue staining, Picro-sirius red staining, Masson-Goldner’s trichrome staining, Movat’s pentachrome staining, von Kossa staining technics to identify different structures in bone and cartilage. The outcomes might help researchers to determine the appropriate staining technics to be used in their studies to fully demonstrate their findings.
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    Sex specific knee joint soft tissue mineralization with fibrillin-1 mutation in male tight skin mice
    (Forum Multimedia Publishing, 2025) Keenan, Craig; Wang, Xia; Dikmen, Tayfun; Wen, Y.; Ramos-Mucci, Lorenzo; Shorter, Emily; Abraham, David; Bou-Gharios, George; Poulet, Blandine
    Articular soft tissue mineralization and ossification are clear pathological signs of osteoarthritis (OA) joints. However their molecular and cellular aetiologies remain largely unknown. Transforming growth factor beta (TGF-β) family members are known contributors to both pathological ossification and osteoarthritis development. In this study, we used a fibrillin-1 (Fbn1) mutant mouse, the tight skin (TSK) mouse, to define the detrimental effects of abnormal Fbn1 in TSK mice and known high TGF-β activity in joint pathology such as articular soft tissue mineralization and ossification. Methods: Knee joints of male and female TSK and wild-type (WT) littermates were analysed by micro-computed tomography (micro-CT) imaging and histology for articular soft tissue pathologies, as well as OA severity. Both aged (10, 26, 35 and 52 weeks) and following in vivo non-invasive repetitive joint overloading were used. Results: We find that male TSK mice develop spontaneous soft tissue ossification from 26 weeks of age, followed by increased osteoarthritis at 1 year-old. In addition, knee joint overloading induced ligament and meniscal mineralisation and ossification in both WT and TSK male mice, but were significantly more severe in TSK knees, including ossification of the patella ligament and synovial lining. In contrast, female TSK knees did not develop more severe soft tissue mineralisation compared to littermate WT mice in neither aged nor overloaded knees. Conclusions: We conclude that Fbn1 mutation, and possibly overactive TGF-β activity in TSK mice, induce articular soft tissue ossification and osteoarthritis in a sex-specific manner. Further studies are needed to confirm the specific signalling involved and the relative protection from female mice from such pathologies.