Histological staining technics for identifying various structures for histological assessment of bone and cartilage healing
Yükleniyor...
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ICBH
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bone and cartilage regeneration are challenging topics which the scientists are constantly looking for new approaches to eliminate current limitations of the traditional therapeutic methods which are being used. Cellular therapy and use of biomaterials for that purpose is providing encouraging results, especially in cases like osteoarthritis and bone healing. In such studies, the histological assessment of the healing of corresponding tissues are vital to determine to see if the developed method is indeed resulted with positive outcomes or not. Each tissue has some different distinctive structures that must be investigated during the healing process. There are several histological staining technics being used for the evaluation of the healing of bone and cartilage tissues by identifying the structures of interest. Among them, hematoxylin eosin staining comes forward as the most applied technic. However, it is not suitable to identify each and every important histological structure, and some specific staining technics are necessary to identify such structures like new bone formation areas, mineralized bone areas, glycosaminoglycan aggregates, collagen fiber formation, etc; regarding the aim of the research. In this study, bone and articular cartilage samples were taken and stained with Alcian blue staining, Picro-sirius red staining, Masson-Goldner’s trichrome staining, Movat’s pentachrome staining, von Kossa staining technics to identify different structures in bone and cartilage. The outcomes might help researchers to determine the appropriate staining technics to be used in their studies to fully demonstrate their findings.
Açıklama
Anahtar Kelimeler
Histological Assessment, Histological Staining, Bone Healing, Cartilage Healing
Kaynak
International Congress on Biological and Health Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Dikmen, T. (2023). Histological staining technics for identifying various structures for histological assessment of bone and cartilage healing. International congress on biological and health sciences, pp. 105. ICBH
