Evaluation of the wound healing potential of volatile oil-loaded nanofibers

Volatile oils possess antimicrobial and anti-inflammatory activities beneficial for wound healing. In time, nanotechnological approaches like nanofibers have been developed to enhance volatile oils’ stability, bioavailability and controlled release [1] Our study aims to develop nanofibers loaded with a mixture of volatile oils to utilize in wound healing. A volatile oil mixture was prepared according to the book of Demirezer et al. [2]. Four volatile oils were used namely; pelargonium, chamomile, immortelle and tea tree oils were used as volatile oils to prepare the mixture. Polyvinylpyrrolidone (PVP) 360 was used for volatile oil-loaded nanofiber production. Formulations with polymer concentration of 15% (w/v) and 1 ml of volatile oil mixture were electrospun. The experimental setup varied in voltage, flow rate and collector distance. GC-MS analyzes of the mixture of oil and nanofibers were conducted. While the microbroth dilution method was applied to both volatile oil and the mixture oil, disc diffusion method used for the nanofiber parts. Strains were Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 [3] [4]. The results of antimicrobial tests are given in Table 1. The nanofibers collected in the collector formed a circle shape with a diameter of 5 cm in the production where a homogeneous fiber throw occurred. Only 4-terpinol, neryl acetate, geraniol and citronellol are found in GC-MS results of nanofibers. When all results were evaluated jointly, mixture volatile oil loaded nanofibers can be promising for wound healing activity however additional experiments should be designed by changing the parameters.