A novel HPLC method for selexipag in human plasma and application to a prototype pharmacokinetic study
dc.authorid | 0000-0002-1871-017X | en_US |
dc.authorid | 0000-0002-5840-7386 | en_US |
dc.authorscopusid | 49964600400 | en_US |
dc.authorscopusid | 6701367993 | en_US |
dc.authorwosid | A-3672-2016 | en_US |
dc.authorwosid | FZV-5400-2022 | en_US |
dc.contributor.author | Ceylan, Burhan | |
dc.contributor.author | Tırıs, Gizem | |
dc.contributor.author | Kepekçi Tekkeli, Şerife Evrim | |
dc.contributor.author | Önal, Cem | |
dc.contributor.author | Önal, Armağan | |
dc.date.accessioned | 2023-12-02T11:44:58Z | |
dc.date.available | 2023-12-02T11:44:58Z | |
dc.date.issued | 2023 | en_US |
dc.department | Fakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı | en_US |
dc.description.abstract | A novel simple and cost effective HPLC technique was presented for the quantification of selexipag (SLP) in human plasma sample and the technique’s applicability to a pharmacokinetic investigation. Chromatographic separation was achieved with C18 (5 μm 3 4.6 mm 3 150 mm) column, at 30 8C with isocratic elution, mobile phase composed of solution A (acetonitrile), and solution B (0.5% formic acid) (65:35 v/v) at flow rate 1.2 mL min 1 . The linearity range is 10–150 ng mL 1 . As sample preparation step human plasma was precipitated with acetonitrile and the detection was provided at 300 nm. The retention time is 8.20 ± 0.02 min. LOD is found to be 3.3 ng mL 1 for drug. The method was applied to the analysis of SLP in human plasma with good recovery as 97.83%. Validation of the studied methods was carried out according to EMA guideline. The new method applied on a prototype pharmacokinetic study by administration of 800 μg SLP to a healthy volunteer and parameters like AUC0–24, AUC0–∞, Cmax, tmax, and t1/2 were assessed. | en_US |
dc.identifier.citation | Ceylan, B., Tırıs, G., Kepekçi Tekkeli, Ş. E., Önal, C., & Önal, A. (2023). A novel HPLC method for selexipag in human plasma and application to a prototype pharmacokinetic study. Acta Chromatographica, 36(3), pp. 273-278. https://doi.org/10.1556/1326.2023.01167 | en_US |
dc.identifier.doi | 10.1556/1326.2023.01167 | en_US |
dc.identifier.endpage | 278 | en_US |
dc.identifier.issn | 1233-2356 | |
dc.identifier.issn | 2083-5736 | |
dc.identifier.issue | 3 | en_US |
dc.identifier.scopus | 2-s2.0-85179043351 | en_US |
dc.identifier.scopusquality | Q3 | en_US |
dc.identifier.startpage | 273 | en_US |
dc.identifier.uri | https://doi.org/10.1556/1326.2023.01167 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13055/591 | |
dc.identifier.volume | 36 | en_US |
dc.identifier.wos | WOS:001308771100011 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak.other | SCI-E - Science Citation Index Expanded | en_US |
dc.institutionauthor | Kepekçi Tekkeli, Şerife Evrim | |
dc.institutionauthor | Önal, Cem | |
dc.language.iso | en | en_US |
dc.publisher | Akademiai Kiado | en_US |
dc.relation.ispartof | Acta Chromatographica | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Selexipag | en_US |
dc.subject | HPLC-UV | en_US |
dc.subject | Pharmacokinetics | en_US |
dc.subject | Human Plasma | en_US |
dc.subject | Pulmonary Arterial Hypertension | en_US |
dc.title | A novel HPLC method for selexipag in human plasma and application to a prototype pharmacokinetic study | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication |
Dosyalar
Orijinal paket
1 - 1 / 1
Yükleniyor...
- İsim:
- A novel HPLC method for selexipag in human plasma and application to a prototype pharmacokinetic study.pdf
- Boyut:
- 594.54 KB
- Biçim:
- Adobe Portable Document Format
- Açıklama:
- Tam Metin / Full Text
Lisans paketi
1 - 1 / 1
Kapalı Erişim
- İsim:
- license.txt
- Boyut:
- 1.44 KB
- Biçim:
- Item-specific license agreed upon to submission
- Açıklama: