An UPLC method for the determination of sorafenib in human plasma by fluorimetric detection with pre-column derivatization and application to a pharmacokinetic study

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dc.authorid0000-0002-5840-7386en_US
dc.authorscopusid6701367993en_US
dc.authorwosidFZV-5400-2022en_US
dc.contributor.authorTırıs, Gizem
dc.contributor.authorKepekçi Tekkeli, Şerife Evrim
dc.contributor.authorÖnal, Cem
dc.contributor.authorCeylan, Burhan
dc.contributor.authorÖnal, Armağan
dc.date.accessioned2022-08-29T12:32:33Z
dc.date.available2022-08-29T12:32:33Z
dc.date.issued2022en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalıen_US
dc.description.abstractThis research presents a new, sensitive and selective UPLC method with fluorometric detection for the determination of sorafenib in human plasma and application of the method to a pharmacokinetic study. Sorafenib was precolumn derivatized with 7-chloro-4-nitrobenzofurazan (NBD-Cl) and the separation of the fluorescent derivative was performed with a C18 column (50 mm x 2.1 mm, 1.7 µm) at 40ºC using a mobile phase composed of acetonitrile - 0.1% trifluoroacetic acid in water (60:40, v/v) by isocratic elution with flow rate of 0.5 mL min−1 . The injection volume was 7 µL. The method depends on the measurement of the derivative using fluorescence detection (λex = 398 nm, λem = 425 nm). The retention time of sorafenib was 3.10 ± 0.02 min. The novel method was validated in accordance with ICH criteria by studying on the parameters such as specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 0.25-10 µg mL−1 with the correlation coefficient of 0.9995. Limit of detection and quantitation were found to be 0.075 and 0.25 µg mL−1 , respectively. Intraday and interday RSD values were less than 5.48%. The plasma concentration–time profile and pharmacokinetic parameters such as AUC0–t , AUC0–∞, Cmax, tmax, t1/2 were measured according to the assays. The proposed method is feasible to investigate the bioequivalence and bioavailability and routine analysis of the drug in plasma.en_US
dc.identifier.citationTırıs, G., Kepekçi Tekkeli, Ş. E., Önal, C., Ceylan, B., & Önal, A. (2022). An UPLC method for the determination of sorafenib in human plasma by fluorimetric detection with pre-column derivatization and application to a pharmacokinetic study. Revue Roumaine de Chimie, 67(3), 217–224. https://doi.org/10.33224/rrch.2022.67.3.10en_US
dc.identifier.doi10.33224/rrch.2022.67.3.10en_US
dc.identifier.endpage224en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85136794323en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage217en_US
dc.identifier.urihttps://doi.org/10.33224/rrch.2022.67.3.10
dc.identifier.urihttps://hdl.handle.net/20.500.13055/258
dc.identifier.volume67en_US
dc.identifier.wosWOS:000863060300011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorÖnal, Cem
dc.language.isoenen_US
dc.publisherEditura Academiei Romaneen_US
dc.relation.ispartofRevue Roumaine de Chimieen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAn UPLC method for the determination of sorafenib in human plasma by fluorimetric detection with pre-column derivatization and application to a pharmacokinetic studyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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