A novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma

Basit Öğe Kaydı
dc.authorid0000-0003-0933-881X
dc.authorid0000-0002-2547-6086
dc.authorid0000-0002-1871-017X
dc.contributor.authorEgeli Yılmaz, Derya
dc.contributor.authorTırıs, Gizem
dc.contributor.authorKepekçi Tekkeli, Şerife Evrim
dc.date.accessioned2026-06-06T12:17:25Z
dc.date.available2026-06-06T12:17:25Z
dc.date.issued2026
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractThis study introduces a combined HPLC and UV detection technique for the quantification of casticin in capsule and human plasma samples. The chromatographic separation was carried out utilizing a C18 column (150 mm × 4.6 mm × 5 μm) at a temperature of 25 ºC. Isocratic elution with a mobile phase comprising 60:40 v/v (methanol-0.05% formic acid) was employed. Flow rate was adjusted 1 mL/min. The analyte was determined at a wavelength of 258 nm, with a retention time of 14.7±0.01 min. The developed method underwent validation according to ICH criteria, covering specificity, linearity, precision, accuracy, detection and quantitation limits, as well as robustness. The linear range was determined to be 10-60 ng/mL for both capsule and spiked plasma specimens. The suggested technique was performed to the analysis of casticin in spiked human plasma and pharmaceutical preparations, yielding a recovery of 106.04% and demonstrating precision through intra-day and inter-day experiments with the highest relative standard deviation (RSD %) value of 4.94. Consequently, the technique was performed to the quantifying of human plasma specimens from in a patient taking medication containing casticin.
dc.identifier.citationEgeli Yılmaz, D., Tırıs, G., & Kepekçi Tekkeli, Ş. E. (2026). A novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. Journal of Research in Pharmacy, 30(3), pp. 826-834. https://doi.org/10.12991/jrespharm.1708765
dc.identifier.doi10.12991/jrespharm.1708765
dc.identifier.endpage834
dc.identifier.issn2630-6344
dc.identifier.issue3
dc.identifier.scopus2-s2.0-105039456606
dc.identifier.scopusqualityQ3
dc.identifier.startpage826
dc.identifier.urihttps://doi.org/10.12991/jrespharm.1708765
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1508
dc.identifier.volume30
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynak.otherESCI - Emerging Sources Citation Index
dc.institutionauthorKepekçi Tekkeli, Şerife Evrim
dc.institutionauthorid0000-0002-1871-017X
dc.language.isoen
dc.publisherMarmara University Press
dc.relation.ispartofJournal of Research in Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCasticin
dc.subjectHigh Performance Liquid Chromatography
dc.subjectValidation
dc.subjectHuman Plasma
dc.subjectCapsule
dc.titleA novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Kapalı Erişim
İsim:
Tam Metin / Full Text.pdf
Boyut:
393.95 KB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Kapalı Erişim
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Analitik Kimya Ana Bilim Dalı Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu