Pharmacokinetic analysis of febuxostat in human serum by UFLC based on fluorimetric derivatization

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dc.authorid0000-0002-5840-7386en_US
dc.authorid0000-0002-1871-017Xen_US
dc.authorscopusid49964600400en_US
dc.authorscopusid6701367993en_US
dc.authorwosidA-3672-2016en_US
dc.authorwosidFZV-5400-2022en_US
dc.contributor.authorBinay, Emrullah
dc.contributor.authorÖnal, Cem
dc.contributor.authorÖnal, Armağan
dc.contributor.authorKepekçi Tekkeli, Şerife Evrim
dc.date.accessioned2024-02-28T11:30:51Z
dc.date.available2024-02-28T11:30:51Z
dc.date.issued2024en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalıen_US
dc.description.abstractAn ultra fast liquid chromatographic (UFLC) method using fluorimetric detection for the quantitation of Febuxostat (FEB) in human serum was developed by precolumn derivatization with 4- bromomethyl-7-methoxy coumarin (BrMmC). The derivatization was catalyzed with dibenzo-18-crown-6-ether to produce a fluorescent derivative which was determined through fluorescence measurement with excitation at 320 nm and emission at 395 nm wavelengths. A C18 column with 100 cm  4.6 mm, 3 µm particule size was used for the UFLC separation procedure. A mixture of acetonitrile: methanol: 0.05 M aqueous ammonium acetate (pH 5.0) (40:40:20, v/v/v) was used as mobile phase. During the procedure, the flow rate was maintained at 0.5 mL/min. A calibration curve was then plotted as 0.005–3.0 µg/mL with a detection limit of 0.0022 µg/mL and quantification limit of 0.0073 µg/mL. The mean recovery to reveal the accuracy and relative standard deviation (RSD) that indicates the precision of the method were found out to be 85.20 % and less than 3.52 %, respectively. The utility of the new method has been demonstrated by measuring the pharmacokinetics of FEB by administration of 80 mg tablets to a healthy female volunteer, aged 42.en_US
dc.identifier.citationBinay, E., Önal, C., Önal, A., & Kepekçi Tekkeli, Ş. E. (2023). Pharmacokinetic analysis of febuxostat in human serum by UFLC based on fluorimetric derivatization. Revue Roumaine de Chimie, 68(10–12), pp. 527–532. https://doi.org/10.33224/rrch.2023.68.10-12.04en_US
dc.identifier.doi10.33224/rrch.2023.68.10-12.04en_US
dc.identifier.endpage532en_US
dc.identifier.issn0035-3930
dc.identifier.issue10-12en_US
dc.identifier.scopus2-s2.0-85185126291en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage527en_US
dc.identifier.urihttps://doi.org/10.33224/rrch.2023.68.10-12.04
dc.identifier.urihttps://hdl.handle.net/20.500.13055/658
dc.identifier.volume68en_US
dc.identifier.wosWOS:001266621800004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorÖnal, Cem
dc.institutionauthorKepekçi Tekkeli, Şerife Evrim
dc.language.isoenen_US
dc.publisherEditions de l'Academie Republique Populaireen_US
dc.relation.ispartofRevue Roumaine de Chimieen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject4-Bromomethyl-7-Methoxy Coumarinen_US
dc.subjectFebuxostaten_US
dc.subjectFluorescence Detectionen_US
dc.subjectHuman Serumen_US
dc.subjectUltra Fast Liquid Chromatographyen_US
dc.titlePharmacokinetic analysis of febuxostat in human serum by UFLC based on fluorimetric derivatizationen_US
dc.typeArticleen_US
dspace.entity.typePublication

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