Electrophoretic approaches for investigating plant-derived compound interactions with dna and proteins

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dc.authorid0009-0009-3405-3905
dc.authorid0009-0007-3387-3202
dc.authorid0009-0001-5000-7863
dc.authorid0000-0001-6151-074X
dc.authorid0000-0002-0967-0997
dc.authorid0000-0001-5228-1682
dc.contributor.authorAydemir, Zeynep
dc.contributor.authorKaya, Beril Sena
dc.contributor.authorBakırcı, Meysera
dc.contributor.authorAl Faysal, Abdullah
dc.contributor.authorKalaycıoğlu, Zeynep
dc.contributor.authorGölcü, Ayşegül
dc.date.accessioned2026-11-09T09:34:44Z
dc.date.available2026-11-09T09:34:44Z
dc.date.issued2026
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractPlant-derived compounds are increasingly recognized as promising therapeutic agents due to their broad biological activities and structural diversity, making their interactions with DNA and proteins central to understanding mechanisms of action and guiding drug discovery. Electrophoretic techniques probe these interactions by tracking changes in electrophoretic mobility, conformation, complex formation, and biopolymer integrity. This review surveys electrophoretic strategies for characterizing plant-derived compound-DNA and -protein interactions, covering fundamental DNA-binding modes (intercalation, groove binding, electrostatic association, cleavage), representative phytochemicals and extracts, and analytical challenges of complex plant matrices, including chemical heterogeneity, synergistic and antagonistic effects, and active-constituent identification. Particular attention is given to capillary electrophoresis-based approaches (affinity CE, CE, and pressure-assisted frontal analysis) and gel-based methods (agarose gel electrophoresis, native PAGE, SDS-PAGE). The literature shows capillary electrophoresis excels at quantifying binding affinity, stoichiometry, and specificity, whereas gel electrophoresis better visualizes ligand-induced changes in DNA topology and protein behavior. HSA and BSA dominate as model proteins, while advanced methods remain underapplied to chemically complex extracts, gaps pointing toward broader protein targets and natural-product systems. Integrating mass spectrometry, advanced detection, and data-driven approaches could further strengthen the mechanistic and quantitative reach of electrophoretic analysis in this field.
dc.identifier.citationAydemir, Z., Kaya, B. S., Bakırcı, M., Al Faysal, A., Kalaycıoğlu, Z., & Gölcü, A. (2026). Electrophoretic approaches for investigating plant-derived compound interactions with dna and proteins. Electrophoresis, pp. 1-23. https://doi.org/10.1002/elps.70163
dc.identifier.doi10.1002/elps.70163
dc.identifier.endpage23
dc.identifier.issn1522-2683
dc.identifier.issn0173-0835
dc.identifier.pmidPMID: 42811817
dc.identifier.scopusqualityQ2
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1002/elps.70163
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1701
dc.identifier.wosWOS:001888379100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorKaya, Beril Sena
dc.institutionauthorid0009-0007-3387-3202
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofElectrophoresis
dc.relation.publicationcategoryDiğer
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgarose Gel Electrophoresis
dc.subjectCapillary Electrophoresis
dc.subjectDNA Interactions
dc.subjectPlant-Derived Compounds
dc.subjectProtein–Ligand Interactions
dc.titleElectrophoretic approaches for investigating plant-derived compound interactions with dna and proteins
dc.typeReview Article
dspace.entity.typePublication

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