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https://hdl.handle.net/20.500.13055/33

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Inactivation of Nosema spp. with zinc phthalacyonine
(Elsevier, 2024) Gök Yurttaş, Asiye; Çınar, Kamil; Khan, Zaeema; Elgün, Tuğba; Mayack, Christopher
Most honey bee pathogens, such as Vairimorpha (Nosema), cannot be rapidly and definitively diagnosed in a natural setting, consequently there is typically the spread of these diseases through shared and re-use of beekeeping equipment. Furthermore, there are no viable treatment options available for Nosema spores to aid in managing the spread of this bee disease. We therefore aimed to develop a new method using novel Zinc Phthalacyonine (ZnPc) as a photosensitizer for the photodynamic inactivation of Nosema spores that could be used for the decontamination of beekeeping equipment. Nosema spores were propagated for in vitro testing using four caged Apis mellifera honey bees. The ZnPc treatment was characterized, encapsulated with a liposome, and then used as either a 10 or 100 µM treatment for the freshly harvested Nosema spores, for either a 30 and or 60-minute time period, under either light or dark conditions, in-vitro, in 96-well plates. In the dark treatment, after 30-min, the ZnPc 100 µM treatment, caused a 30 % Nosema mortality, while this increased to 80 % at the same concentration after the light treatment. The high rate of anti-spore effects, in a short period of time, supports the notion that this could be an effective treatment for managing honey bee Nosema infections in the future. Our results also suggest that the photo activation of the treatment could be applied in the field setting and this would increase the sterilization of beekeeping equipment against Nosema.
Stevioside improves brain oxidant-antioxidant status in overfed zebrafish
(Istanbul University Press, 2023) Dandin, Esra; Ünal, İsmail; Beler, Merih; Üstündağ, Ünsal Veli; Cansız, Derya; Ateş Kalkan, Perihan Seda; Emekli-Alturfan, Ebru
Objective: An excessive buildup of adipose tissue is a defining feature of overnutrition, and a significant fraction of the world’s population suffers from obesity. Overnutrition is associated with the deterioration of mitochondrial functions in the brain in the case of obesity. In this study, we evaluated the effects of stevioside (ST) which is a calorie-free, naturally occurring herbal sweetener made from Stevia rebaudiana (Bertoni) on the oxidant-antioxidant balance in the brain in cases of overnutrition. Accordingly, the effects of ST consumption on the oxidant-antioxidant balance in the brain was evaluated and determined in a case study of overfeeding adult zebrafish for 15 days. Materials and Methods: Zebrafish were placed in four groups; the control group (C); overfed group (OF); low-dose (1mg/L) ST treated OF group (OF+LDS); and the high-dose ST (5mg/L) treated OF group (OF+HDS). The levels of lipid peroxidation (LPO) were evaluated together with nitric oxide (NO) to determine the oxidant status. The antioxidant status from the activities of the superoxide dismutase (SOD) and glutathione S-transferase (GST) were determined in brain tissues. Results: The ST treatment decreased the increased LPO and NO levels in overfed zebrafish and increased SOD and GST activities in a dose-dependent manner. Conclusion: ST exerted an antioxidant effect on the possible damage mechanisms that could occur in the brain in case of overnutrition by decreasing oxidative stress and improving the antioxidant enzyme activities.
Evaluation of serum placenta-specific gene 8 protein, total antioxidant capacity, interleukin-10, interleukin-17A, interleukin-21 and interleukin-33 levels in Turkish women with gestational diabetes mellitus
(Elsevier, 2024) Çıracı, Enver; Elgün, Tuğba; Gök Yurttaş, Asiye; Çağın Kuzey, Hazel; Ekenoğlu Merdan, Yağmur; Toprak, Muhammed Sait; Tetik, Sermin
Purpose: Gestational diabetes mellitus (GDM) is defined as carbohydrate intolerance that begins or is diagnosed during pregnancy. Our study aimed to establish a correlation between proin flammatory and anti-inflammatory response in order to be able to develop treatment strategies and determine early diagnosis biomarkers in the sera of cases diagnosed with GDM. Moreover, we aimed to investigate interleukin (IL), placenta-specific gene 8 protein (PLAC8) and total antioxidant capacity (TAC) in patients with GDM. Methods: A total of 121 patients were included in the study. These were divided into four patient groups: pregnant and diagnosed with DM (P-GDM, n = 30); pregnant and not diagnosed with DM (P-NGDM, n = 32); non-pregnant diagnosed with DM (NP-DM, n = 29) and non-pregnant and not diagnosed with DM (NPNDM, n = 30). IL-10, IL-17A, IL-21, IL-33, PLAC8 and TAC determinations from patients were evaluated by ELISA (Enzyme-Linked ImmunoSorbent Assay) method. Results: IL-10 and IL-33 concentrations were found to be significantly higher in P-GDM and NP DM patient groups compared to P-NGDM and NP-NDM groups (p < 0.001). The PLAC8 level in the P GDM patient group (20.38 ± 5.37) was determined to be significantly higher than in the P-NGDM patient group (3.41 ± 2.17, p < 0.001). TAC in the P-NGDM and NP-NDM groups (12.42 ± 2.31 vs. 12.96 ± 3.78, p < 0.001) was determined to be significantly higher than in the P-GDM and NP-DM groups (4.8 ± 0.52 vs. 2.21 ± 0.71, p < 0.001). Discussion: The fact that the importance of PLAC8 level and TAC in the diagnosis and follow-up of GDM in pregnancy is demonstrated for the first time in this study shows that it is unique.
The protective role of vitamins C and E in steroid-induced femoral head osteonecrosis: An experimental study in rats
(Turkish Joint Diseases Foundation, 2024) Beytemur, Ozan; Daşçı, Mustafa Fatih; Gök Yurttaş, Asiye; Yaprak Bayrak, Büşra; Alagöz, Ender
Objectives: This study aimed to determine whether vitamin C (VC) and vitamin E (VE) can effectively protect the femoral head and reduce the risk of developing osteonecrosis in rats that have been treated with steroids.Materials and methods: The study was conducted on 30 young adult male Sprague-Dawley rats (mean weight: 356 +/- 18 g; range, 330 to 375 g), which were randomly assigned to one of five groups. The control group received saline solution, while the other groups were given lipopolysaccharide/methylprednisolone (LPS/MPS) to induce osteonecrosis. Three groups in which osteonecrosis was induced were also intraperitoneally administered either VC, VE, or both once a day for four weeks. Intracardiac blood samples were taken at the end of the fourth week for biochemical examination, and the rats were then sacrificed under general anesthesia. After sacrification, right femurs were removed for histopathological, immunohistochemical, and radiologic examinations.Results: The results showed that the mean trabecular number increased significantly in the VC+VE group. There was a substantial decrease observed in the mean trabecular separation within the LPS/MPS group compared to the control group, although trabecular separation decreased in all three vitamin groups compared to the LPS/MPS group. The surface area/bone volume was significantly increased in the VC+VE group compared to the LPS/MPS group. Histological, immunohistochemical, and radiological examinations showed that the administration of VC and VE significantly reduced oxidative stress, inflammation, and microvascular dysfunction in rats with steroid-induced femoral head osteonecrosis.Conclusion: This study suggests that VC, VE, and particularly VC+VE have a protective effect on the femoral head in rats with steroid-induced femoral head osteonecrosis. These findings may lead to new treatment options for patients.
Synergistic effects of ozone with doxorubicin on the proliferation, apoptosis and metastatic profile of luminal-B type human breast cancer cell line
(Elsevier, 2023) Karagülle, Onur Olgaç; Gök Yurttaş, Asiye
Objective Luminal-B type human breast cancer cell line (BT-474) to assess the synergistic effects of ozone applied after chemotherapeutic treatment with various dosages of doxorubicin, and compare the results with the effects on L929 fibroblast cell line. Methods Doxorubicin (1–50 M) was added to each cell lines and left to sit for 24 h at 37 °C. Then, as combination groups, half of the groups were incubated with 30 g/mL ozone for 25 min. Tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and matrix metalloproteinase-2 and − 9 (MMP-2 and MMP-9) levels were measured using the MTT test, flow cytometry, and immunocytochemistry, respectively. Results When compared to simply doxorubicin-applied cells without ozone treatment, each dose of doxorubicin + ozone treatment considerably boosted L929 viability but significantly decreased BT-474 viability. Additionally, the combination increased the apoptotic impact of doxorubicin on BT-474 but not L929 (P < 0.001). TGF-, MMP-2, and MMP-9 levels of L929 after combination were substantially higher than those of the other groups (P < 0.01). Doxorubicin's effect on BT-474's protein levels, which had significantly decreased in comparison to those of the other groups, was reversed by the combination treatment (P < 0.05). Conclusion Doxorubicin's anti-proliferative and apoptotic effects were enhanced by ozone treatment in BT-474 cells, but it also repaired and healed healthy fibroblast cells that had been harmed by the cytotoxicity of the chemotherapy drug. If doxorubicin and ozone treatment are coupled, BT-474 cells may develop resistance to it through expressions of TNF-α, TGF-β, MMP-2, and MMP-9.
Effect of Aza-BODIPY-photodynamic therapy on the expression of carcinoma-associated genes and cell death mode
(Elsevier, 2023) Elgün, Tuğba; Gök Yurttaş, Asiye; Çınar, Kamil; Özçelik, Şennur; Gül, Ahmet
Background: Breast cancer is the most common cancer affecting women worldwide.Photodynamic therapy(PDT) has now proven to be a promising form of cancer therapy due to its targeted and low cytotoxicity to healthy cells and tissues.PDT is a technique used to create cell death localized by light after application of a light-sensitive agent.Aza-BODIPY is a promising photosensitizer for use in PDT. Our results showed that aza-BODIPY-PDT induced apoptosis, probably through p53 and caspase3 in MCF-7 cells. Future studies should delineate the molecular mechanisms underlying aza-BODIPY-PDT-induced cell death for a better understanding of the signaling pathways modulated by the therapy so that this novel technology could be implemented in the clinic for treating breast cancer. Aim: In this study,we aimed to determine the change in the expression levels of 88 carcinoma-associated genes induced by aza-BODIPY-PDT were analyzed so as to understand the specific pathways that are modulated by aza-BODIPY-PDT. Material method: In this study,the molecular basis of the anti-cancer activity of aza-BODIPY-PDT was investigated.Induction of apoptosis and necrosis in MCF-7 breast cancer cells after treatment with aza- BODIPY derivative with phthalonitrile substituents (aza-BODIPY) followed by light exposure was evaluated by Annexin V 7- Aminoactinomycin D (7-AAD) flow cytometry. Results: Aza-BODIPY-PDT induced cell death in MCF-7 cells treated with aza-BODIPY-PDT; flow cytometry revealed that 28% of the cells died by apoptosis. Seven of the 88 carcinoma-associated genes that were assayed were differentially expressed -EGF, LEF1, WNT1, TCF7, and TGFBR2 were downregulated, and CASP3 and TP53 were upregulated - in cells subjected to aza-BODIPY-PDT.This made us think that the aza-BODIPY-PDT induced caspase 3 and p53-mediated apoptosis in MCF7 cells. Conclusion: In our study,it was determined that the application of aza-BODIPY-PDT to MCF7 cells had a negative effect on cell connectivity and cell cycle.The fact that the same effect was not observed in control cells and MCF7 cells in the dark field of aza-BODIPY indicates that aza-BODIPY has a strong phodynamic anticancer effect.
Glycosylated zinc(II) phthalocyanine photosensitizer: Synthesis, photophysical properties and in vitro photodynamic activity on breast cancer cell line
(Elsevier, 2024) Bilen, Başak Sezgi; Özçeşmeci, Mukaddes; Koçyiğit, Nilüfer; Elgün, Tuğba; Yurttaş, Asiye Gök; Hamuryudan, Esin
Breast cancer is a common type of cancer among women worldwide. Triple negative breast cancer is a highly aggressive subtype of breast cancer and accounts for approximately 24% of all identified breast cancer cases. In triple negative breast cancer, the therapeutic approach options are very limited. In this study, 4-[2′,3′,5′,6′-tetrafluoro-4′-(1,2:5,6-di-O-isopropylidene-α-d-glucofuranosyl)benzyloxy]phthalonitrile and its peripherally tetra substituted zinc phthalocyanine derivatives are reported for the first time. Photophysical and photochemical properties of the glycosylated zinc(II) phthalocyanine derivative were investigated for its suitability for use as a photosensitizer in photodynamic therapy. The fluorescence (ΦF: 0.089), singlet oxygen (Φd: 0.45) and photodegradation quantum yields (Φ∆: 6.07×10−5) of this compound were carried out in N,N-dimethylformamide. The in vitro photodynamic activities of this compound against MDA-MB-231 cells were also studied. Cytotoxicity analysis in this cell lines was performed by MTT (3-(4,5-dimethylthiazol-2-yl) method. Cytotoxicity test was applied on cell lines of MDA-MB-231 and HUVEC. The IC50 value is determined for glycosylated zinc(II) Pc derivative.
Vitamin B kompleksinin fotodinamik terapiyle kolon kanserine etkisi
(Süleyman Demirel Üniversitesi, 2023) Gök Yurttaş, Asiye; Çınar, Kamil
Amaç: Kolon kanserleri, hastanın yaşam kalitesini derinden etkileyen ve çeşitli komplikasyonlarla ilişkili kronik ölümcül bir hastalıktır. Vitamin B kompleksi, gıda takviyesi olarak alındığında olumlu anti-inflamatuar etkiler göstermektedir. Bu çalışma, fotodinamik terapiyle lipozomlu vitamin B kompleksinin kolon kanserlerinde (HCT-116 ve HT-29 hücre hattı) anti-kanser etkisini araştırmayı amaçlamıştır. Materyal- Metot: Çalışmanın birinci basamağını oluşturan Vitamin B kompleksi lipozom ile kapsüle edildi. Lipozomlu vitamin B kompleksi Zetasizer cihazı ile karakterize edildi. Lipozomlu vitamin B kompleksi MTT (3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür) testi ile HCT-116 ve HT-29 hücre hattında % canlılık oranlarına bakılmıştır. Aynı hücre hatlarında vitamin B kompleksinin fotodinamik terapi etkisiyle hangi ölüm yolaklarını seçtiğini göstermek için de akış sitometrisi kullanılarak analiz edilmiştir. Bulgular: Lipozomlu vitamin B kompleksinin boyutları 100 nm ile 200 nm aralığında değiştiği belirlendi. Lipozomlu vitamin B kompleksi (fotosensitizer) 7,5 J/cm2 ışık dozunda 0,25 mg/ mL'de karanlık deneyinde HCT-116 hücre canlılık yüzdesi %75 iken ışık deneyi sonrası bu oran %47’lere düşmüştür (****p<0.001). 0,5 mg/mL vitamin B kompleksi çözeltisi için yapılan karanlık deneyinde HT-29 hücre canlılık yüzdesi %70 iken ışık deneyi sonrası bu oran %35’lere düşmüştür (****p<0.001). Arzu edilen fotodinamik etkinlik bu sonuçlara daha yakındır. Hücre ölüm yolakları belirleme deneyinde lipozomlanmış vitamin B kompleksinin konsantrasyonu 1mg/mL olarak HCT-116 ve HT-29 hücrelerine uygulanmıştır. HCT-116 hücre hattında %40 oranında apoptoz görülürken HT-29 hücre hattında %60 oranında apoptoz görüldü. Sonuç: Pazarlanan ilaçları yeniden konumlandırmak, yeni antitümör ilaçları keşfetmek için yeni bir yaklaşım olarak ortaya çıkmıştır. Pazarlanan ilaçlar için yeni kullanım alanlarının geliştirilmesi, yeni anti-tümör ilaçları geliştirmenin etkili ve hızlı yolu olduğu kanıtlanmıştır. Verilerimiz in-vitro olarak bazı kolon kanser türlerinde vitamin B kompleksinin fotodinamik terapiyle kullanımının ümit vaat ettiğini savunmaktadır.
Exploring the molecular mechanism of linagliptin in osteosarcoma cell lines for anti-cancer activity
(Elsevier, 2023) Gök Yurttaş, Asiye; Daşçı, Mustafa Fatih
Background: Finding new applications for widely used current drugs is a fast and effective technique for discovering new anticancer chemicals. Osteosarcoma (OS), the most prevalent form of bone cancer, has several side effects that significantly lower patients' quality of life. This study aims to systematically examine the anti-cancer activity of linagliptin (LG) in the osteosarcoma cell line Saos-2. Methods: MTT assays and flow cytometry were used to assess cell viability and apoptosis, respectively. qPCR array experiments were carried out to determine target gene expressions and explain the molecular mechanism of LG's action. Results: Linagliptin treatment significantly decreased the viability of Saos-2 cells and hFOB1.19 cells (p < 0.001). The treatment also induced increased apoptotic effects in both Saos-2 cells (p < 0.001) and hFOB1.19 cells (p < 0.05). qPCR assays were conducted to assess cancer pathway analysis after applying specific quantities of LG to Saos-2 and hFOB1.19 cells. Conclusion: The findings of this study demonstrate that LG inhibits the proliferation of Saos-2 cells and induces cell death. LG supports cell death by suppressing the expression of specific genes involved in cancer pathways.
Determination of bisphenol a and phthalate levels in wastewater samples
(İstanbul Üniversitesi, 2022) Akçay, Mansur; Ateş Kalkan, Perihan Seda; Üstündağ, Ünsal Veli; Ünal, İsmail; Cansız, Derya; Emekli-Alturfan, Ebru; Alturfan, Ahmet Ata
Objective: The use of endocrine-disrupting chemicals (EDCs) such as bisphenol A (BPA) in plastics manufacturing, agriculture, livestock, and paint manufacturing increas daily. The water treated in wastewater treatment plants is used in many areas such as irrigation of parks and gardens, and reinforcement of underground water resources. However, whether the treatment process eliminates EDCs in wastewater is not exactly known, and determining this as well as the amounts of these chemicals in treated water are important in terms of protecting the environment and human health. The aim of the study was to determine BPA and phthalate concentrations in the influent and effluent flow samples obtained from wastewater treatment plants. Materials and Methods: BPA and phthalate concentrations were measured in influent and effluent flow samples using the enzyme- linked immunosorbent assay (ELISA) method. BPA and phthalate measurements were performed as competitive measurements of BPA and total phthalates in samples using specific monoclonal antibodies. Results: BPA and phthalate levels were measured respectively as 7.69 μg/L and 78.27 μg/L in the influent water samples and 3.17 μg/L and 25.56 μg/L in the effluent water samples. The concentration of BPA and phthalates in the effluent samples decreased significantly compared to the influent water samples. Conclusion: This study is believed to shed light on the importance of monitoring BPA and phthalate concentrations in wastewater treatment plants and inspections for detecting other EDCs in wastewater.
Determination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkey
(Elsevier, 2023) Elgün, Tuğba; Müşteri Oltulu, Yasemin; Yurttaş Gök, Asiye; Aygüz, Umut; Kılıç, Ulkan
Introduction: Choosing the right clinical approach for early and reliable diagnosis/screening is becoming more important day by day. The aim of the study was to determine the early RhD type with cff-DNA obtained from maternal plasma, especially in the light of recent developments. In this way, it is aimed to apply Rh Ig only to mothers who are determined to have RhD (+) fetuses and to prevent unnecessary further tests that may possess a risk for RhD (-) fetuses. Methods: Prediction of fetal gender and RH genotype was performed by using RT-qPCR method. With simultaneous amplification of sequences of SRY, DYS14 and RH genes (exon 7 and exon 10). Fetal gender and RhD were determined in 30 RHD (-) pregnant women with cfDNA. Results: As a result of genotyping, the gender of 67% (20/30) fetuses was determined as male; the gender of 33% (10/30) fetuses was determined as female in a sample group of 30 pregnancies. It was determined that the DYS14 100% (20/20) gene was more sensitive than the SRY 97% (18/20) gene in gender determination after examining prenatal and postnatal results. As a result of the analysis, the presence of 17% (5/30) RhD (-) fetuses and 83% (25/30) RhD (+) fetuses were determined which is 100% compatible with postnatal results. Discussion: Detecting fetal RhD gene in maternal plasma made an important contribution to its use in non-invasive prenatal screening. This study shows that unnecessary intervention and cost can be avoided with successful genotyping analysis performed with RT-qPCR.
Photodyanamic therapy in the cervical cancer
(Özgür Yayınları, 2023) Gök Yurttaş, Asiye; Gök Yurttaş, Asiye
Cancer is one of the most common and feared illnesses in modern society. Its prevalence has been gradually rising in recent years. The fourth most common malignancy in women worldwide is cervical cancer (CC). Surgery, chemotherapy, and radiotherapy are examples of conventional treatments, but they are invasive and have negative side effects. In addition, metastasis is observed in roughly 70% of individuals with late-stage CC due to limits and treatment resistance. In addition to treating primary CC, photodynamic therapy (PDT) is an alternate CC treatment strategy that has been clinically shown to reduce subsequent metastasis. Because PDT is a non-invasive focused treatment with less adverse effects and reduced resistance to dose repetitions, it is believed to be much more beneficial. This review study’s objective is to examine the studies on PDT’s efficacy in treating cervical cancer.
Novel targeted therapeutic approaches to cancers
(Özgür Yayınları, 2023) Gök Yurttaş, Asiye
Kanser, modern toplumun en yaygın ve korkulan hastalıklarından biridir. Son yıllarda insidansı giderek artmaktadır. Kanserin moleküler özelliklerinin anlaşılmaya başlamasıyla birlikte çalışmalar hedefe yönelik tedaviler üzerine yoğunlaşmıştır. Bu kitabın amacı ise kanserlere yeni hedefli terapötik yaklaşimlara ilişkin literatürü analiz etmektir. Bu kitap temel bilim, kanserlere özgü moleküler yaklaşımlar ve sinyal yolakları, hedefli ilaçlar, klinik araştırmalar ve psikoterapi gibi alanları kapsamaktadır. Kanser veya çoklu stres tepkileri birçok insan, hayvan ve bitki hastalığının altında yatmaktadır ve bu da kitap içeriğimizin önemli bir bileşenini oluşturmaktadır. Bu kitabın konu yelpazesi, kanserle ilişkili hastalıklar dünya çapında hızla ilerledikçe büyük bir güncelliğe sahip olacaktır.
Genetic deviation associated with photodynamic therapy in hela cell
(Elsevier, 2023) Gök Yurttaş, Asiye; Okat, Zehra; Elgün, Tuğba; Uçar Çifçi, Kezban; Sevim, Altuğ Mert
Photodynamic therapy (PDT) is a crucial method that is used in cancer treatment. Entering the program for PDT selection today gives a new direction. The main therapeutic effect is the production of singlet oxygen (1O2), which activates the photosensitizer (PS). Phthalocyanines for PDT applications are preferred because they produce high singlet oxygen with absorbers of about 600-700 nm. Aim It is aimed to analyze cancer cell pathways by flow cytometry analysis and cancer-related genes with q-PCR device by applying phthalocyanine L1ZnPC, which we use as photosensitizer in photodynamic therapy, in HELA cell line. In this study, we investigate the molecular basis of L1ZnPC's anti-cancer activity. Material method The cytotoxic effects of L1ZnPC, a phthalocyanine obtained from our previous study, in HELA cells were evaluated and it was determined that it led to a high rate of death as a result. The result of photodynamic therapy was analyzed using q-PCR. From the data received at the conclusion of this investigation, gene expression values were calculated, and expression levels were assessed using the 2−∆∆Ct method to examine the relative changes in these values. Cell death pathways were interpreted with the FLOW cytometer device. One-Way Analysis of Variance (ANOVA) and the Tukey-Kramer Multiple Comparison Test with Post-hoc Test were used for the statistical analysis. Conclusion In our study, it was observed that HELA cancer cells underwent apoptosis at a rate of 80% with drug application plus photodynamic therapy by flow cytometry method. According to q-PCR results, CT values of eight out of eighty-four genes were found to be significant and their association with cancer was evaluated. L1ZnPC is a new phthalocyanine used in this study and our findings should be supported by further studies. For this reason, different analyses are needed to be performed with this drug in different cancer cell lines. In conclusion, according to our results, this drug looks promising but still needs to be analyzed through new studies. It is necessary to examine in detail which signaling pathways they use and their mechanism of action. For this, additional experiments and animal studies are required.
Molecular mechanisms of heat shock proteins in distinct diseases
(Scientific Scholar, 2023) Yürekli, Nazlıcan; Tutar, Merve; Niyazov, Laziz; Sağır, Fatma; Açıkalın Coşkun, Kübra; Al, Mervenur; Uçar Çifçi, Kezban; Abay, Cansu; Gök Yurttaş, Asiye; Okat, Zehra; Tutar, Yusuf; Asea, Alexzander A. A.; Kaur, Punit
Heat shock proteins (HSPs) modulate the molecular mechanics of cells and cellular systems. Properly folded proteins are required for cellular processes and organisms use molecular mechanics to keep substrate proteins in their native state. Therefore, cellular compartments employ redundant isoforms of HSP to maintain substrate protein homeostasis and keep them functional. Hence, HSP folder function is critical for cell, HSPs are universally conserved. HSPs play essential roles in cellular signalling and in immune system besides their folder function. To this purpose, the roles of distinct HSP are described in this review to describe their molecular mechanisms in diseases.
Kolorektal kanserde kullanılan biyobelirteçler
(Kolorektal Cerrahi Hastalıklar, 2020) Gök Yurttaş, Asiye; Akbaş, Ahmet
Kolorektal kanser (KK), hem erkek hem de kadınlarda meydana gelen en yaygın ve ölümcül malignitelerden biri olarak kabul edilir. Yaygın prevalansı, KK için yeni tanısal ve prognostik biyobelirteçlere olan ihtiyacı göstermektedir. Aynı hastalık, farklı bireylerde çok farklı klinik seyir gösterebilmektedir. Bu sürecin önceden tahmin edilebilmesi, tedavi etkinliğinin belirlenebilmesi, istenmeyen yan etkilerin azaltılabilmesi, hasta için optimal tedavi seçeneğinin öngörülebilmesinde kullanılan biyobelirteçlerin önemi artmıştır. Burada, kolorektal karsinomların, taranma, tanı, izlem ve prognozunun belirlenmesinde kullanılan biyobelirteçler ele alınmış, günümüzden geleceğe doğru değişmekte olan biyobelirteç perspektifi çıkarılmaya çalışılmıştır.
P. aeruginosa bakterisinin vitamin B kompleksi ve kırmızı lazer kullanılarak inaktive edilmesi
(Süleyman Demirel Üniversitesi, 2022) Gök Yurttaş, Asiye; Çınar, Kamil
Son yıllarda anti-bakteriyel direnç olgusu, bakterilerin neden olduğu hastalıklarla mücadelede daha fazla sorun haline gelmiştir. Biz bu çalışmayla, açık yara ve yanıkların fırsatçı patojeni olan Pseudomonas aeruginosayı daha etkili bir yöntemle inaktive etmeyi ve fotodinamik inaktivasyon(PDI) gelişimine katkıda bulunmayı hedefledik. Pseudomonas aeruginosa bakterisini, vitamin B kompleksi ve lazer ile inaktive olduğunu spektrofotometrik ölçümler ve antimikrobiyal madde etkinliği analiz yöntemleriyle gösterdik. Ayrıca PDI'nin bakteri üremesi üzerindeki etkisi hem kalitatif hem de kantitatif olarak değerlendirilmiştir. Escherichia coli ve Pseudomonas aeruginosa suşları karşılaştırılarak ölüm oranları belirlendi. Koloni oluşturma birimi oranlarında Escherichia coli bakteri suşlarının karanlık ve ışık deneyinde vitamin B kompleksinin 0,25 mg/mL ve 0,125 mg/mL konsantrasyonlarında % 20’lik bir ölüm oranı görülmüştür. Pseudomonas aeruginosa bakteri suşlarının karanlık deneyinde vitamin B kompleksinin 0,5 mg/mL ve 0,125 mg/mL konsantrasyonlarında çok az bir ölüm oranı görülürken lazer maruziyet sonrası ölüm oranları sırasıyla % 60 ve % 50 çıktığı görülmüştür. Bu ölüm oranlarını doğrulama amaçlı akış sitometresi canlılık deneyleri yapılmış ve çıkan sonuçlar birbiriyle paralellik göstermiştir. Bu çalışmadan elde edilen veriler ışığında; kırmızı lazer diyotun, vitamin B kompleksi ile birlikte Pseudomonas aeruginosa bakterisinin inaktivasyonu için uygun bir aday olduğunu öngörmekteyiz. Yaptığımız bu çalışma, bakteriyel enfeksiyonların tedavisi için hastane, tıp ve mikrobiyoloji alanlarında yapılacak alternatif tedavi yöntemlerine ışık tutacaktır.
Role of p53 in human cancers
(IntechOpen, 2022) Açıkalın Çoşkun, Kübra; Tutar, Merve; Al, Mervenur; Gök Yurttaş, Asiye; Abay, Elif Cansu; Yürekli, Nazlıcan; Yeman Kıyak, Bercem; Uçar, Kezban; Tutar, Yusuf; Anwar, Mumtaz
TP53 codes tumor protein 53-p53 that controls the cell cycle through binding DNA directly and induces reversible cell-cycle arrest. The protein activates DNA repair genes if mutated DNA will be repaired or activates apoptotosis if the damaged DNA cannot be fixed. Therefore, p53, so-called the “guardian of the genome,” promote cell survival by allowing for DNA repair. However, the tumor-suppressor function of p53 is either lost or gained through mutations in half of the human cancers. In this work, functional perturbation of the p53 mechanism is elaborated at the breast, bladder, liver, brain, lung cancers, and osteosarcoma. Mutation of wild-type p53 not only diminishes tumor suppressor activity but transforms it into an oncogenic structure. Further, malfunction of the TP53 leads accumulation of additional oncogenic mutations in the cell genome. Thus, disruption of TP53 dependent survival pathways promotes cancer progression. This oncogenic TP53 promotes cell survival, prevents cell death through apoptosis, and contributes to the proliferation and metastasis of tumor cells. The purpose of this chapter is to discuss the contribution of mutant p53 to distinct cancer types.
The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
(Turkish Joint Diseases Foundation, 2022) Daşçı, Mustafa Fatih; Yaprak Saraç, Elif; Gök Yurttaş, Asiye; Atçı, Tolgahan; Uslu, Muhammed; Acar, Abdurrahman; Güleç, Mehmet Akif; Alagöz, Ender
Objectives: In this study, we aimed to evaluate the antioxidant, antiapoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. Materials and methods: A total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/ methylprednisolone (LPS/MPS) group administered 20 μg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed. Results: Micro-computed tomography showed a higher bone mineral density and lower porosity, Tr.Sp and Tr.Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20-deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum. Conclusion: Vascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and antiapoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis.
Biyolojik tanıma elemanlarından proteinoidler kullanılarak empedans ölçüm yaklaşımıyla bakterilerin tespiti
(Bilecik Şeyh Edebali Üniversitesi, 2021) Gök Yurttaş, Asiye
Günümüz dünyasında gelişen teknoloji ile su, gıda ve çevresel kirliliğin artmasıyla bu temel ihtiyaçların sağlık kontrolü oldukça zorlayıcıdır. Bu tür eksiklerin giderilmesi üzerine bir çalışma olmuştur. Bu amaçla iki temel aşamalı bir iş planı oluşturulmuştur. Birincisi; hedef bakteriye seçici tepki veren biyomoleküllerden olan proteinoidler sentezlenip karakterize edilmiştir. Şuana kadar kullanılan biyolojik tanıma elemanlarından ilk defa farklı olarak proteinoidler bu çalışma ile kullanılmıştır. İkinci aşamada ise proteinoidler, yayılım gösteren bakterileri seçici olarak algılayarak elektriksel empedans ile fiziksel parametrelerde değişime neden olarak bioelektrik dönüşümü sağlanmasıdır. Dönüştürücü (transducer) içerisinde elektriksel karakterizasyon yöntemleri ile bakteri uyarımlarında teşhis başarımı elde edilmiştir.

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