Exploring the molecular mechanism of linagliptin in osteosarcoma cell lines for anti-cancer activity
Yükleniyor...
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Finding new applications for widely used current drugs is a fast and effective technique for discovering new anticancer chemicals. Osteosarcoma (OS), the most prevalent form of bone cancer, has several side effects that significantly lower patients' quality of life. This study aims to systematically examine the anti-cancer activity of linagliptin (LG) in the osteosarcoma cell line Saos-2. Methods: MTT assays and flow cytometry were used to assess cell viability and apoptosis, respectively. qPCR array experiments were carried out to determine target gene expressions and explain the molecular mechanism of LG's action. Results: Linagliptin treatment significantly decreased the viability of Saos-2 cells and hFOB1.19 cells (p < 0.001). The treatment also induced increased apoptotic effects in both Saos-2 cells (p < 0.001) and hFOB1.19 cells (p < 0.05). qPCR assays were conducted to assess cancer pathway analysis after applying specific quantities of LG to Saos-2 and hFOB1.19 cells. Conclusion: The findings of this study demonstrate that LG inhibits the proliferation of Saos-2 cells and induces cell death. LG supports cell death by suppressing the expression of specific genes involved in cancer pathways.
Açıklama
Anahtar Kelimeler
Anti-Cancer, HFOB1.19, Linagliptin, Osteosarcoma, QPCR, Saos-2
Kaynak
Pathology Research and Practice
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
248
Sayı
Künye
Gök Yurttaş, A., & Daşçı, M. F. (2023). Exploring the molecular mechanism of linagliptin in osteosarcoma cell lines for anti-cancer activity. Pathology Research and Practice, 248, pp. 1-6. https://doi.org/10.1016/j.prp.2023.154640
