Quantification of agnuside in human plasma with a novel high-performance liquid chromatographic method and pharmacokinetic study

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dc.authorid0000-0003-0933-881X
dc.authorid0000-0002-2547-6086
dc.authorid0000-0002-1871-017X
dc.contributor.authorEgeli, Derya
dc.contributor.authorTiris, Gizem
dc.contributor.authorKepekçi Tekkeli, Şerife Evrim
dc.date.accessioned2025-01-30T18:09:28Z
dc.date.available2025-01-30T18:09:28Z
dc.date.issued2025
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractThis study presents a combination of High Performance Liquid Chromatography (HPLC) and ultraviolet (UV) detection that provides the quantification of agnuside in human plasma specimens. Reverse-phase chromatographic separation was carried out with C18 column (150 mm × 4.6 mm × 5 μm), at 25°C with isocratic elution of the mobile phase containing methanol: 0.1% formic acid (35:65 v/v) at 0.6 mL/min flow rate. Experiments were carried out at a wavelength of 258 nm. The retention time of the analyte is 9.70 ± 0.01 min. The developed technique was validated based on the International Conference on Harmonization guideline. The correlation coefficient of the technique was 0.9915, and the calibration range was 5–125 μg/mL. The recovery value of the proposed method was found to be 101.4%, and the precision of the method was calculated as 6.35 with the highest RSD% value. A pharmacokinetic study was performed by administering agnuside to a healthy volunteer.
dc.identifier.citationEgeli, D., Tiris, G., & Kepekçi Tekkeli, Ş. E. (2025). Quantification of agnuside in human plasma with a novel high-performance liquid chromatographic method and pharmacokinetic study. Journal of Chromatographic Science, 63(2), pp. 1-6. https://doi.org/10.1093/chromsci/bmaf007
dc.identifier.doi10.1093/chromsci/bmaf007
dc.identifier.endpage6
dc.identifier.issn1945-239X
dc.identifier.issn0021-9665
dc.identifier.issue2
dc.identifier.pmid39835650
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1093/chromsci/bmaf007
dc.identifier.urihttps://hdl.handle.net/20.500.13055/909
dc.identifier.volume63
dc.identifier.wosWOS:001400632000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorKepekçi Tekkeli, Şerife Evrim
dc.institutionauthorid0000-0002-1871-017X
dc.language.isoen
dc.publisherOxford University Press
dc.relation.ispartofJournal of Chromatographic Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectVıtex-Negundo
dc.subjectRotundıfolıa
dc.subjectConstıtuents
dc.titleQuantification of agnuside in human plasma with a novel high-performance liquid chromatographic method and pharmacokinetic study
dc.typeArticle
dspace.entity.typePublication

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