Development of an HPLC method for the determination of mesalazine in human plasma by fluorimetric derivatization and application to a prototype pharmacokinetic study

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dc.authorid0000-0002-5840-7386en_US
dc.authorscopusid6701367993en_US
dc.authorwosidFZV-5400-2022en_US
dc.contributor.authorCeylan, Burhan
dc.contributor.authorKepekçi Tekkeli, Evrim
dc.contributor.authorÖnal, Cem
dc.date.accessioned2021-12-10T13:16:00Z
dc.date.available2021-12-10T13:16:00Z
dc.date.issued2022en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalıen_US
dc.description.abstractIn this study, a new, fast and sensitive HPLC method with fluorometric detection was developed for the determination of mesalazine in human plasma and applied to a pharmacokinetic study. Mesalazine was precolumn derivatized with NBD-Cl and the fluorescent derivative was separated on a C18 (150 x 4.6 mm x 2.6 mu m) analytical column at 30 oC using a mobile phase composed of acetonitrile-0.1% o-phosphoric acid in water (70:30, v/v) by isocratic elution with flow rate of 1.0 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection (lambda(ex) = 280 nm, lambda(em) = 325 nm). The retention time of mesalazine is 3.08 +/- 0.06 min. Nortriptiline was used as internal standard. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 0.25-1.5 mu g mL(-1) with the correlation coefficient of 0.9997. LOD and LOQ were found to be 0.075 and 0.25 mu g mL(-1), respectively. Intraday and interday RSD values were less than 5.92%. The plasma concentration-time profile and pharmacokinetic parameters such as AUC(0-t), AUC(0-infinity), C-max, t(max), t(1/2), were calculated according to the assays. The presented method can certainly be used for bioequivalence and bioavailability investigations and routine analysis of the drug in plasma.en_US
dc.identifier.citationCeylan, B., Tekkeli, E. K. & Önal, C. (2022). Development of An HPLC Method for the Determination of Mesalazine in Human Plasma by Fluorimetric Derivatization and Application to A Prototype Pharmacokinetic Study. Journal of Fluorescence, 32(1), pp. 319-325. https://doi.org/10.1007/s10895-021-02848-4en_US
dc.identifier.doi10.1007/s10895-021-02848-4en_US
dc.identifier.endpage325en_US
dc.identifier.issue1en_US
dc.identifier.pmidPMID: 34797459en_US
dc.identifier.scopus2-s2.0-85119526304en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage319en_US
dc.identifier.urihttps://doi.org/10.1007/s10895-021-02848-4
dc.identifier.urihttps://hdl.handle.net/20.500.13055/79
dc.identifier.volume32en_US
dc.identifier.wosWOS:000720588700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorÖnal, Cem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Fluorescenceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMesalazineen_US
dc.subjectHPLC-FLen_US
dc.subjectPre-column Derivatizationen_US
dc.titleDevelopment of an HPLC method for the determination of mesalazine in human plasma by fluorimetric derivatization and application to a prototype pharmacokinetic studyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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