Novel dual-fluorescent flow cytometric approach for quantification of macrophages infected with Leishmania infantum parasites

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dc.authorid0000-0003-4789-9095en_US
dc.authorscopusid57212405586en_US
dc.contributor.authorİşlek, Zeynep
dc.contributor.authorÜçışık, Mehmet Hikmet
dc.contributor.authorŞahin, Fikrettin
dc.date.accessioned2021-12-24T12:23:40Z
dc.date.available2021-12-24T12:23:40Z
dc.date.issued2022en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Teknoloji Bölümü, Farmasötik Teknoloji Ana Bilim Dalıen_US
dc.description.abstractFlow cytometry analysis emerges as an alternative methodology to microscopy for determination of the Leishmania-infection rates of macrophages. Various flow cytometric approaches have been established for the quantification of Leishmania parasites within host cells, labelled either directly fluorescent dyes or indirectly with fluorescently conjugated antibodies. Although these techniques allow accurate quantification of infection, they fail at detection of non-infected macrophages specifically. This study introduces a new flow cytometric approach for the determination of infection rates of macrophages infected by Leishmania infantum parasites. Prior to infection, J774A.1 macrophages and L. infantum promastigotes were stained separately with PKH26 and PKH67 dyes, respectively. Dual staining enabled detection of each cell type, where non-infected macrophages were also recorded for the quantification. Dual-PKH staining achieved high success in selective staining of promastigotes (99.71%) and macrophages (99.57%). The percentages of parasite-infected macrophages were determined for initial 1:2.5 and 1:10 infection ratios as 15.68 and 61.70%, respectively; indicating significant increase in infection rate parallel to the initial treatment ratio. These results demonstrated that the introduced dual-fluorescence flow cytometric approach can be successfully used as an accurate and rapid quantification method for L. infantum-infected macrophages and strengthens the hypothesis that flow cytometric approaches could replace conventional microscopic methodologies.en_US
dc.identifier.citationİşlek, Z., Üçışık, M., & Şahin, F. (2022). Novel dual-fluorescent flow cytometric approach for quantification of macrophages infected with Leishmania infantum parasites. Parasitology, 149(1), pp. 44-50. doi:10.1017/S0031182021001530en_US
dc.identifier.doi10.1017/S0031182021001530en_US
dc.identifier.endpage50en_US
dc.identifier.issue1en_US
dc.identifier.pmidPMID: 34488918en_US
dc.identifier.scopus2-s2.0-85114945790en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage44en_US
dc.identifier.urihttps://doi.org/10.1017/S0031182021001530
dc.identifier.urihttps://hdl.handle.net/20.500.13055/116
dc.identifier.volume149en_US
dc.identifier.wosWOS:000758044900007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorİşlek, Zeynep
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.relation.ispartofParasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDetection of Leishmania Infectionen_US
dc.subjectLeishmania Infantumen_US
dc.subjectPKH Dyesen_US
dc.subjectFlow Cytometryen_US
dc.subjectFluorescent Assayen_US
dc.subjectMacrophagesen_US
dc.titleNovel dual-fluorescent flow cytometric approach for quantification of macrophages infected with Leishmania infantum parasitesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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