Investigation of the effects of melatonin on granulosa cell proliferation and DNA methylation

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dc.authorid0009-0000-2544-9718
dc.authorid0000-0002-9438-6113
dc.authorid0009-0001-4172-4748
dc.authorid0000-0002-6424-7411
dc.authorid0000-0002-1311-6892
dc.contributor.authorTürkmen, Ervanur
dc.contributor.authorGündoğan, Gül İpek
dc.contributor.authorArslan, Halil İbrahim
dc.contributor.authorGök Yurttaş, Asiye
dc.contributor.authorElgün, Tuğba
dc.date.accessioned2025-11-20T09:55:15Z
dc.date.available2025-11-20T09:55:15Z
dc.date.issued2025
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalı
dc.description.abstractMelatonin, a pineal hormone with antioxidant and regulatory functions, has emerged as a key modulator of ovarian physiology. Its presence in fol licular fluid suggests important roles in granulosa cell function, follicle devel opment, and reproductive outcomes. However, its effects on granulosa cell tumour (GCT) biology and epigenetic regulation remain insufficiently defined. This study aimed to investigate the effects of melatonin on proliferation and global DNA methylation in human granulosa tumour cells (COV434) com pared with healthy endothelial controls (HUVECs). COV434 and HUVEC cells were treated with melatonin at 1, 10, 100, and 1000 µM. Cell viabil ity and proliferation were assessed using the MTT [3-(4.5-dimethylthiazol-2- yl)-diphenyl tetrazolium bromide] (Cambridge, UK) assay and xCELLigence RTCA system (Roche), while DNA methylation was quantified with a 5-mC ELISA kit (Epigentek Group Inc, USA). Experimental groups included nega tive, sham, melatonin-treated, and positive controls. Melatonin showed a cell type-dependent effect. In COV434 cells, proliferation was significantly inhib ited, with an IC50 of 10.55 µM, whereas HUVECs displayed increased prolifer ation at higher doses. DNA methylation levels decreased in both cell types in a dose-dependent manner, reaching the highest significance in COV434 cells at 1000 µM (p < 0.001). In conclusion, melatonin demonstrated a dose-dependent inhibitory effect on COV434 cell proliferation while simultaneously reducing global DNA methylation levels.
dc.identifier.citationTürkmen, E., Gündoğan, G. İ., Arslan, H. İ., Gök Yurttaş, A., & Elgün, T. (2025). Investigation of the effects of melatonin on granulosa cell proliferation and DNA methylation. Comptes Rendus de L'Academie Bulgare des Sciences, 78(10), pp. 1463-1470. https://doi.org/10.7546/CRABS.2025.10.05
dc.identifier.doi10.7546/CRABS.2025.10.05
dc.identifier.endpage1470
dc.identifier.issn1310-1331
dc.identifier.issue10
dc.identifier.scopusqualityQ4
dc.identifier.startpage1463
dc.identifier.urihttps://doi.org/10.7546/CRABS.2025.10.05
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1192
dc.identifier.volume78
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorGök Yurttaş, Asiye
dc.institutionauthorid0000-0002-6424-7411
dc.language.isoen
dc.publisherProceedings of the Bulgarian Academy of Sciences
dc.relation.ispartofComptes Rendus de L'Academie Bulgare des Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMelatonin
dc.subjectGranulosa Cell
dc.subjectProliferation
dc.subjectDNA Methylation
dc.titleInvestigation of the effects of melatonin on granulosa cell proliferation and DNA methylation
dc.typeArticle
dspace.entity.typePublication

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