Thermo, pH, and ionic-strength-responsive MPEG–PEI copolymer: A reversible soluble–insoluble support promotes glucoamylase immobilization and sustainable catalysis
| dc.authorid | 0000-0002-6657-9663 | |
| dc.authorid | 0000-0001-9750-1028 | |
| dc.contributor.author | Haykır, Nazife Işık | |
| dc.contributor.author | Kosaoğlu, Hacim | |
| dc.contributor.author | Tuncel, Ali | |
| dc.contributor.author | Çelebi, Serdar Suut | |
| dc.date.accessioned | 2026-04-01T13:08:19Z | |
| dc.date.available | 2026-04-01T13:08:19Z | |
| dc.date.issued | 2026 | |
| dc.department | Fakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Kimya Mühendisliği Bölümü | |
| dc.description.abstract | Glucoamylase (GA) plays a crucial role in the saccharification of starch and other related oligo saccharides in the food and fermentation industries. The construction of an immobilized GA using a reversibly soluble/insoluble methoxy polyethylene glycol–polyethyleneimine (MPEG–PEI) copolymer synthesized via carbodiimide chemistry is presented in this work. Various MPEG:GA ratios were assessed to optimize enzyme loading and catalytic activity. Immobilized GA retained up to 12.6% of its theoretical activity but demonstrated excellent operational stability and reusability. Substrate-assisted immobilization, using maltose and maltodextrin to protect GA's active site during synthesis of the carrier, significantly improved enzyme activity—up to a 3.1- fold increase compared to the unprotected system. While maltodextrin yielded higher initial activity, maltose with a lower initial activity offered better long-term stability, maintaining 70% of its initial activity even after 11 reuse cycles. Kinetic analysis using the Lambert-W function revealed increased Km for the immobilized enzyme, indicating decreased substrate affinity due to a possible conformational change on the immobilized form of GA. While a minor decrease in conversion efficiency was observed under high initial substrate concentrations in practice, the reversibly soluble/insoluble MPEG–PEI copolymer exhibited effective thermo-responsive char acteristics, enabling the sustained and recyclable use of GA in starch hydrolysis and offering considerable potential for other future biotechnological applications. | |
| dc.identifier.citation | Haykır, N. I., Kosaoğlu, H., Tuncel, A., & Çelebi, S. S. (2026). Thermo, pH, and ionic-strength-responsive MPEG–PEI copolymer: A reversible soluble–insoluble support promotes glucoamylase immobilization and sustainable catalysis. Biocatalysis and Agricultural Biotechnology, 73, pp. 1-12. https://doi.org/10.1016/j.bcab.2026.103987 | |
| dc.identifier.doi | 10.1016/j.bcab.2026.103987 | |
| dc.identifier.endpage | 12 | |
| dc.identifier.issn | 1878-8181 | |
| dc.identifier.scopus | 2-s2.0-105031378847 | |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.startpage | 1 | |
| dc.identifier.uri | https://doi.org/10.1016/j.bcab.2026.103987 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.13055/1373 | |
| dc.identifier.volume | 73 | |
| dc.identifier.wosquality | Q2 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.indekslendigikaynak.other | ESCI - Emerging Sources Citation Index | |
| dc.institutionauthor | Çelebi, Serdar Suut | |
| dc.institutionauthorid | 0000-0001-9750-1028 | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.relation.ispartof | Biocatalysis and Agricultural Biotechnology | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | Glucoamylase | |
| dc.subject | Immobilization | |
| dc.subject | Thermo-Responsive Polymer | |
| dc.subject | Biocatalysis | |
| dc.subject | Enzyme Stability | |
| dc.title | Thermo, pH, and ionic-strength-responsive MPEG–PEI copolymer: A reversible soluble–insoluble support promotes glucoamylase immobilization and sustainable catalysis | |
| dc.type | Article | |
| dspace.entity.type | Publication |
