Investigation of the effects of fluoride varnish, silver diamine fluoride and peptide P11-4 on dentin nanostructure in an in-vitro dentin caries model via SEM, FTIR spectroscopy and SAXS methods

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dc.authorid0000-0002-2476-0122
dc.authorid0000-0003-1228-1260
dc.authorid0000-0003-1893-4058
dc.authorid0000-0002-4453-3867
dc.contributor.authorYoğurucu Değerli, Gizem
dc.contributor.authorGüven Polat, Günseli
dc.contributor.authorİde, Semra
dc.contributor.authorBülbül, Onurcan
dc.date.accessioned2025-11-22T11:39:27Z
dc.date.available2025-11-22T11:39:27Z
dc.date.issued2025
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümü, Pedodonti Ana Bilim Dalı
dc.description.abstractBackground: To evaluate and compare the structural effects of fluoride varnish, silver diamine fluoride (SDF), and peptide P11-4 on dentin nanostructure in an in vitro dentin caries model. Methods: Forty dentin discs were demineralized and treated with either SDF, fluoride varnish or P11-4. Structural changes were assessed using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and small angle X-ray scattering (SAXS) to evaluate topographical, molecular and nanoscale modifications. Results: SEM revealed morphological differences across groups: the P11-4 group showed fibrillar structures and narrowed dentinal tubules; the SDF group exhibited blocked tubules with a granular appearance; and the fluoride varnish group presented partially occluded tubules. FTIR analysis showed a reduction in Amide A and Amide I bands in the P11-4 group, suggesting enhanced interaction with dentin collagen and early-stage remineralization. In contrast, the SDF group showed higher Amide A values, indicating limited interaction with the organic matrix. These spectral shifts imply differential impacts on the preservation and reorganization of the dentin matrix. SAXS analysis confirmed that the P11-4 group exhibited the closest nanostructural resemblance to healthy dentin, whereas the SDF group showed the least similarity. Both the P11-4 and fluoride varnish groups demonstrated organized fibrillar alignment and improved mineral patterning. Conclusions: The findings suggest that P11-4, through its biomimetic action, facilitates favorable nanostructural and molecular changes in demineralized dentin. These effects may contribute to enhanced mechanical stability and long-term clinical outcomes. Broader in vivo studies are warranted to validate these results before clinical application.
dc.description.sponsorshipThis work was supported by the Sağlık Bilimleri Univer sity Scientific Research Coordination Unit (Project Number: 2022/027) and is part of the PhD thesis of Dr. Gizem Yoğurucu Değerli. Bu çalışma Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Koordinasyon Birimi tarafından desteklenmiştir (Proje Numarası: 2022/027) ve Dr. Gizem Yoğurucu Değerli'nin doktora tezinin bir parçasıdır.
dc.identifier.citationYoğurucu Değerli, G., Güven Polat, G., İde, S., & Bülbül, O. (2025). Investigation of the effects of fluoride varnish, silver diamine fluoride and peptide P11-4 on dentin nanostructure in an in-vitro dentin caries model via SEM, FTIR spectroscopy and SAXS methods. Journal of Clinical Pediatric Dentistry, 49(6), pp. 215-224. https://doi.org/10.22514/jocpd.2025.144
dc.identifier.doi10.22514/jocpd.2025.144
dc.identifier.endpage224
dc.identifier.issn1053-4628
dc.identifier.issn1557-5268
dc.identifier.issue6
dc.identifier.scopusqualityQ2
dc.identifier.startpage215
dc.identifier.urihttps://doi.org/10.22514/jocpd.2025.144
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1200
dc.identifier.volume49
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorYoğurucu Değerli, Gizem
dc.institutionauthorid0000-0002-2476-0122
dc.language.isoen
dc.publisherMRE Press
dc.relation.ispartofJournal of Clinical Pediatric Dentistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiomimetics
dc.subjectCaries
dc.subjectDentin
dc.subjectSelf-Assembling Peptide
dc.subjectPreventive Dentistry
dc.titleInvestigation of the effects of fluoride varnish, silver diamine fluoride and peptide P11-4 on dentin nanostructure in an in-vitro dentin caries model via SEM, FTIR spectroscopy and SAXS methods
dc.typeArticle
dspace.entity.typePublication

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