Therapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model

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dc.authorid0000-0002-2530-7012
dc.authorid0000-0001-7933-4262
dc.authorid0000-0003-3302-5004
dc.authorid0000-0003-1526-2065
dc.authorid0000-0002-8662-5284
dc.authorid0000-0003-4451-7442
dc.authorid0000-0002-7059-1884
dc.contributor.authorŞerefoğlu, Ege Can
dc.contributor.authorKolbaşı, Bircan
dc.contributor.authorBülbül, Muhammet Volkan
dc.contributor.authorKarabulut, Seda
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorGündoğdu Özdemir, Reyhan Zeynep
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2024-10-09T07:50:26Z
dc.date.available2024-10-09T07:50:26Z
dc.date.issued2024
dc.departmentFakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
dc.description.abstractPurpose: This study aimed to examine the therapeutic effects of injection of conditioned medium of adipose-derived mesen chymal stem cells (ADMSC-CM) in a surgically created varicocele model in comparison with varicocelectomy. Materials and Methods: Twenty-eight male Wistar Albino rats were randomly divided into four groups: sham group, varico cele group, varicocelectomy group, and ADMSC-CM injection group. Sperm parameters were analyzed in samples taken from the epididymis after treatment. Malondialdehyde and superoxide dismutase (SOD) levels in blood samples were exam ined by biochemical analysis. The testicular tissues were stained with hematoxylin-eosin for histological examination (John sen’s Score). Additionally, Western Blot analyzes were performed to detect Claudin-11 levels, the functional protein of the blood-testis barrier, in testicular tissues. Results: Varicocelectomy and ADMSC-CM treatments significantly improved mean sperm parameters (concentration, pro gressive motility, motility, normal sperm morphology) (p≤0.05 for all). Both treatment groups had increased SOD levels along with a decrease in malondialdehyde levels (p≤0.05 for all). No significant difference was observed between the ADMSC CM group and the varicocelectomy group in preserving normal testicular histology according to Johnsen’s Score (p=0.114). Levels of Claudin-11 were significantly higher in the varicocelectomy and ADMSC-CM groups compared to the varicocele group (p≤0.05 for all). Conclusions: The therapeutic effects of ADMSC-CM in varicocele model may involve secretion of anti-inflammatory and re generative factors from ADMSC. ADMSC-CM injection appears to be a promising new strategy in the treatment of varicocel
dc.description.sponsorshipIstanbul Medipol University - BAP22-35
dc.identifier.citationŞerefoğlu, E. C., Kolbaşı, B., Bülbül, M. V., Karabulut, S., Çakıcı, Ç., Gündoğdu Özdemir, R. Z., & Keskin, İ. (2024). Therapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model. The World Journal of Men's Health. https://doi.org/10.5534/wjmh.240059
dc.identifier.doi10.5534/wjmh.240059
dc.identifier.issn2287-4208
dc.identifier.issn2287-4690
dc.identifier.pmidPMID: 39344116
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.5534/wjmh.240059
dc.identifier.urihttps://hdl.handle.net/20.500.13055/818
dc.identifier.wosWOS: 001321200800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorGündoğdu Özdemir, Reyhan Zeynep
dc.institutionauthorid0000-0003-4451-7442
dc.language.isoen
dc.publisherKorean Society for Sexual Medicine and Andrology
dc.relation.ispartofThe World Journal of Men's Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectClaudins
dc.subjectCulture Media
dc.subjectConditioned
dc.subjectMesenchymal Stem Cells
dc.subjectInfertility
dc.subjectVaricocele
dc.titleTherapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model
dc.typeArticle
dspace.entity.typePublication

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