Magnetic micro solid-phase extraction for a novel UHPLC-DAD method for the determination of atomoxetine (ATX) in breast milk and human plasma
Yükleniyor...
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academia Română
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Atomoxetine is a special pharmaceutics used for attention deficit hyperactivity disorder. It finds application in various age groups, including children, adolescents, and adults. Ultra high-performance liquid chromatographic technique (UHPLC) is an exceptional technique and provides swift, uncomplicated, and highly sensitive results. Such a technique has been formulated to analyse atomoxetine levels in both breast milk and human plasma. Magnetic micro solid phase extraction is simple, rapid, efficient, and precise method which was applied prior to chromatographic separation. In this method, magnetic adsorbents in the form of Fe3O4 magnetic nanoparticles were employed, and the adsorption process underwent optimization. Chromatographic separation was carried out using a reversed-phase C18 analytical column (5 μm × 4.6 mm × 150 mm) with a mobile phase composed of monobasic potassium dihydrogen orthophosphate (pH=6.8) and acetonitrile (50:50 v/v). The flow rate was set at 0.8 mL/min, and investigation was performed using DAD at 215±2 nm. The method's linearity was evaluated within the range of 0.5–20 μg/mL, achieving a correlation coefficient (r) of 0.999. Validation of the method encompassed accuracy, reproducibility, precision, robustness, specificity, quantification limits, and detection limits, adhering to EMA guidelines. The limit of detection (LOD) was found to be 0.03, while the limit of quantification (LOQ) was 0.11 μg/mL for both matrices. Interday and intraday relative standard deviation (RSD) values were determined to be below 2.5% for both assays. The suggested method is deemed to be a useful candidate for the conventional quantification of atomoxetine in human spiked breast milk and plasma.
Açıklama
Anahtar Kelimeler
Atomoxetine, Magnetic Micro Solid Phase Extraction, Human Plasma, Breast Milk, UHPLC-DAD
Kaynak
Revue Roumaine de Chimie
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
69
Sayı
7-8
Künye
Ceylan, B., Kurnaz Yetim, N., Özcan, C., Koç, M. M., & Önal, C. (2024). Magnetic micro solid-phase extraction for a novel UHPLC-DAD method for the determination of atomoxetine (ATX) in breast milk and human plasma. Revue Roumaine de Chimie, 69(7–8), pp. 423–432.
