Magnetic micro solid-phase extraction for a novel UHPLC-DAD method for the determination of atomoxetine (ATX) in breast milk and human plasma

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dc.authorid0000-0001-6227-0346
dc.authorid0000-0003-4500-0373
dc.authorid0000-0002-5840-7386
dc.contributor.authorCeylan, Burhan
dc.contributor.authorKurnaz Yetim, Nurdan
dc.contributor.authorÖzcan, Cemile
dc.contributor.authorKoç, Mümin Mehmet
dc.contributor.authorÖnal, Cem
dc.date.accessioned2024-10-30T09:16:21Z
dc.date.available2024-10-30T09:16:21Z
dc.date.issued2024
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractAtomoxetine is a special pharmaceutics used for attention deficit hyperactivity disorder. It finds application in various age groups, including children, adolescents, and adults. Ultra high-performance liquid chromatographic technique (UHPLC) is an exceptional technique and provides swift, uncomplicated, and highly sensitive results. Such a technique has been formulated to analyse atomoxetine levels in both breast milk and human plasma. Magnetic micro solid phase extraction is simple, rapid, efficient, and precise method which was applied prior to chromatographic separation. In this method, magnetic adsorbents in the form of Fe3O4 magnetic nanoparticles were employed, and the adsorption process underwent optimization. Chromatographic separation was carried out using a reversed-phase C18 analytical column (5 μm × 4.6 mm × 150 mm) with a mobile phase composed of monobasic potassium dihydrogen orthophosphate (pH=6.8) and acetonitrile (50:50 v/v). The flow rate was set at 0.8 mL/min, and investigation was performed using DAD at 215±2 nm. The method's linearity was evaluated within the range of 0.5–20 μg/mL, achieving a correlation coefficient (r) of 0.999. Validation of the method encompassed accuracy, reproducibility, precision, robustness, specificity, quantification limits, and detection limits, adhering to EMA guidelines. The limit of detection (LOD) was found to be 0.03, while the limit of quantification (LOQ) was 0.11 μg/mL for both matrices. Interday and intraday relative standard deviation (RSD) values were determined to be below 2.5% for both assays. The suggested method is deemed to be a useful candidate for the conventional quantification of atomoxetine in human spiked breast milk and plasma.
dc.identifier.citationCeylan, B., Kurnaz Yetim, N., Özcan, C., Koç, M. M., & Önal, C. (2024). Magnetic micro solid-phase extraction for a novel UHPLC-DAD method for the determination of atomoxetine (ATX) in breast milk and human plasma. Revue Roumaine de Chimie, 69(7–8), pp. 423–432.
dc.identifier.doi10.33224/rrch.2024.69.7-8.10
dc.identifier.endpage432
dc.identifier.issn0035-3930
dc.identifier.issue7-8
dc.identifier.scopus2-s2.0-85209227221
dc.identifier.scopusqualityQ4
dc.identifier.startpage423
dc.identifier.urihttps://doi.org/10.33224/rrch.2024.69.7-8.10
dc.identifier.urihttps://hdl.handle.net/20.500.13055/836
dc.identifier.volume69
dc.identifier.wosWOS:001343292800010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorÖnal, Cem
dc.institutionauthorid0000-0002-5840-7386
dc.language.isoen
dc.publisherAcademia Română
dc.relation.ispartofRevue Roumaine de Chimie
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAtomoxetine
dc.subjectMagnetic Micro Solid Phase Extraction
dc.subjectHuman Plasma
dc.subjectBreast Milk
dc.subjectUHPLC-DAD
dc.titleMagnetic micro solid-phase extraction for a novel UHPLC-DAD method for the determination of atomoxetine (ATX) in breast milk and human plasma
dc.typeArticle
dspace.entity.typePublication

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