A novel differential diagnosis algorithm for chronic lymphocytic leukemia using immunophenotyping with flow cytometry

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dc.authorid0000-0002-0335-2330en_US
dc.authorscopusid57478196200en_US
dc.authorwosidHIR-7894-2022en_US
dc.contributor.authorNarlı Özdemir, Zehra
dc.contributor.authorFalay, Mesude
dc.contributor.authorParmaksız, Ayhan
dc.contributor.authorGenç, Eylem
dc.contributor.authorBeyler, Özlem
dc.contributor.authorGüneş, Ahmet Kürşad
dc.contributor.authorCeran, Funda
dc.contributor.authorDağdaş, Simten
dc.contributor.authorÖzet, Gülsüm
dc.date.accessioned2022-02-28T07:53:10Z
dc.date.available2022-02-28T07:53:10Z
dc.date.issued2023en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik Ana Bilim Dalıen_US
dc.description.abstractIntroduction: The availability of a clinical decision algorithm for diagnosis of chronic lymphocytic leukemia (CLL) may greatly contribute to the diagnosis of CLL, particularly in cases with ambiguous immunophenotypes. Herein we propose a novel differential diagnosis algorithm for the CLL diagnosis using immunophenotyping with flow cytometry. Methods: The hierarchical logistic regression model (Backward LR) was used to build a predictive algorithmfor the diagnosis of CLL, differentiated from other lymphoproliferative disorders (LPDs). Results: A total of 302 patients, of whom 220 (72.8%) had CLL and 82 (27.2%), B-cell lymphoproliferative disorders other than CLL, were included in the study. The Backward LR model comprised the variables CD5, CD43, CD81, ROR1, CD23, CD79b, FMC7, sIg and CD200 in the model development process. The weak expression of CD81 and increased intensity of expression in markers CD5, CD23 and CD200 increased the probability of CLL diagnosis, (p < 0.05). The odd ratio for CD5, C23, CD200 and CD81 was 1.088 (1.050 - 1.126), 1.044 (1.012 - 1.077), 1.039 (1.007 - 1.072) and 0.946 (0.921 - 0.970) [95% C.I.], respectively. Our model provided a novel diagnostic algorithm with 95.27% of sensitivity and 91.46% of specificity. The model prediction for 97.3% (214) of 220 patients diagnosed with CLL, was CLL and for 91.5% (75) of 82 patients diagnosed with an LPD other than CLL, was others. The cases were correctly classified as CLL and others with a 95.7% correctness rate. Conclusions: Our model highlighting 4 markers (CD81, CD5, CD23 and CD200) provided high sensitivity and specificity in the CLL diagnosis and in distinguishing of CLL among other LPDs.en_US
dc.identifier.citationÖzdemir, Z. N., Falay, M., Parmaksız, A., Genç, E., Beyler, O., Güneş, A. K., Ceran, F., Dağdaş, S., & Özet, G. (2023). A novel differential diagnosis algorithm for chronic lymphocytic leukemia using immunophenotyping with flow cytometry. Hematology, Transfusion and Cell Therapy, 45(2), pp. 176-181. https://doi.org/10.1016/j.htct.2021.08.012en_US
dc.identifier.doi10.1016/j.htct.2021.08.012en_US
dc.identifier.endpage181en_US
dc.identifier.issue2en_US
dc.identifier.pmidPMID: 35216960en_US
dc.identifier.scopus2-s2.0-85125817368en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage176en_US
dc.identifier.urihttps://doi.org/10.1016/j.htct.2021.08.012
dc.identifier.urihttps://hdl.handle.net/20.500.13055/164
dc.identifier.volume45en_US
dc.identifier.wosWOS:001019359500001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorParmaksız, Ayhan
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofHematology, Transfusion and Cell Therapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronic Lymphocytic Leukemiaen_US
dc.subjectFlow Cytometryen_US
dc.subjectHierarchical Logistic Regression Modelen_US
dc.titleA novel differential diagnosis algorithm for chronic lymphocytic leukemia using immunophenotyping with flow cytometryen_US
dc.typeArticleen_US
dspace.entity.typePublication

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