Overexpression of CDC25A, AURKB, and TOP2A genes could be an important clue for luminal a breast cancer

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dc.authorid0000-0003-2241-7088
dc.authorid0000-0003-2869-6620
dc.authorid0000-0003-1954-4190
dc.authorid0009-0008-9041-1028
dc.authorid0009-0000-0481-5555
dc.authorid0000-0001-9333-6926
dc.authorid0000-0002-9435-009X
dc.authorid0000-0002-9420-4543
dc.authorid0000-0002-8809-7462
dc.contributor.authorKaya, Murat
dc.contributor.authorAbuaisha, Asmaa
dc.contributor.authorSüer, İlknur
dc.contributor.authorAlptekin, Melike Sultan
dc.contributor.authorAbanoz, Fahrünnisa
dc.contributor.authorEmiroğlu, Selman
dc.contributor.authorPalanduz, Şükrü
dc.contributor.authorCefle, Kıvanç
dc.contributor.authorÖztürk, Şükrü
dc.date.accessioned2024-10-01T13:49:36Z
dc.date.available2024-10-01T13:49:36Z
dc.date.issued2024
dc.departmentFakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
dc.description.abstractObjective: Breast cancer (BC) is highly heterogeneous and one of the most common cancers. Luminal A (LUM A) is a subtype of BC with a better prognosis than other BC subtypes. The molecular mechanisms underlying the initiation and progression of the LUM A subtype are still unclear. Big data generated from microarray and sequencing systems can be re-analyzed, especially with the help of various in silico tools developed in recent years, and made applicable for in vitro and in vivo research. This work aimed to identify genes that may play a role in the progression of LUM A subtype of BC using both computational and laboratory-based methods. Materials and Methods: Overlapping genes associated with BC were identified from the The Cancer Genome Atlas database, GSE233242, GSE100925 geodata sets, and the geneshot tool. The network functional analysis between overlapping genes was determined with STRING 12.0. Expression levels of overlapping genes in BC were investigated with the TNMplot (https://tnmplot.com/analysis/) in silico tool. The effect of overlapping genes on the overall survival of LUM A cancer patients was defined using the Kaplan-Meier plotter tool. Expressions of genes identified using bioinformatics data were investigated via quantitative real-time -polymerase chain reaction (qRT-PCR) in LUM A tumor and adjacent tissue samples. The data were evaluated using the t-test. Both the sensitivity and specificity of selected genes have been determined using the receiver operating characteristic curve. Results: In silico investigation showed that eleven genes were possibly associated with BC. Among them CDC25A, AURKB, and TOP2A were considerably increased in LUM A samples according to qRT-PCR results. An overall survival analysis also showed that overexpression of these three genes could reduce the overall survival of LUM A patients. Conclusion: The genes CDC25A, AURKB, and TOP2A may play crucial functions in LUM A pathogenesis. Therapeutic strategies that diminish the expression of these connected genes may enhance the prognosis of LUM A patients.
dc.identifier.citationKaya, M., Abuaisha, A., Süer, İ., Alptekin, M. S., Abanoz, F., Emiroğlu, S., Palanduz, Ş., Cefle, K., & Öztürk, Ş. (2024). Overexpression of CDC25A, AURKB, and TOP2A genes could be an important clue for luminal a breast cancer. European Journal of Breast Health, 20(4), 284–291. https://doi.org/10.4274/ejbh.galenos.2024.2024-4-3
dc.identifier.doi10.4274/ejbh.galenos.2024.2024-4-3
dc.identifier.endpage291
dc.identifier.issn2587-0831
dc.identifier.issue4
dc.identifier.pmidPMID: 39323324
dc.identifier.scopus2-s2.0-85207951960
dc.identifier.scopusqualityQ2
dc.identifier.startpage284
dc.identifier.trdizinid1276851
dc.identifier.urihttps://doi.org/10.4274/ejbh.galenos.2024.2024-4-3
dc.identifier.urihttps://hdl.handle.net/20.500.13055/812
dc.identifier.volume20
dc.identifier.wosWOS:001330424900008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynak.otherESCI - Emerging Sources Citation Index
dc.institutionauthorAlptekin, Melike Sultan
dc.institutionauthorid0009-0008-9041-1028
dc.language.isoen
dc.publisherGalenos Publishing House
dc.relation.ispartofEuropean Journal of Breast Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBioinformatics
dc.subjectCDC25A
dc.subjectAURKB
dc.subjectTOP2A
dc.subjectLuminal A
dc.titleOverexpression of CDC25A, AURKB, and TOP2A genes could be an important clue for luminal a breast cancer
dc.typeArticle
dspace.entity.typePublication

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