Evaluating a pathogen-specific IgG binding assay for rapid detection of healthcare-associated infections

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dc.authorid0000-0002-7117-5102
dc.authorid0000-0001-9140-2731
dc.authorid0000-0001-8735-5725
dc.authorid0000-0001-6915-9843
dc.authorid0000-0002-3122-5099
dc.contributor.authorKarakullukçu, Asiye
dc.contributor.authorAkker, Mustafa
dc.contributor.authorKuşkucu, Mert Ahmet
dc.contributor.authorAygün, Gökhan
dc.contributor.authorDikmen, Yalım
dc.date.accessioned2026-04-03T14:25:01Z
dc.date.available2026-04-03T14:25:01Z
dc.date.issued2026
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractRapid and accurate diagnosis of healthcare-associated infections (HAIs) is an unmet need for improving outcomes in intensive care units (ICUs). Traditional culture-based methods, while the gold standard, are time-consuming and can delay therapeutic interventions. In this study, we evaluated the diagnostic utility of pathogen-specific immunoglobulin G (IgG) binding levels using an enzyme-linked immunosorbent assay (ELISA). We measured IgG binding against pathogens including Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Enterococcus faecium, and Staphylococcus aureus. Diagnostic performance was evaluated through receiver operating characteristic (ROC) curve analysis, with culture results as the reference. The assay demonstrated an overall diagnostic accuracy of 83.2%, with a sensitivity of 85.4%, a specificity of 81.4%, and an area under the curve (AUC) of 0.910. Pathogen-specific cutoff values ranged from 0.918 to 1.534. Especially, A. baumannii showed the highest performance metrics, achieving a sensitivity of 94.7%, a specificity of 93.6%, and an AUC of 0.975. The pathogen-specific IgG binding levels can offer a novel and effective diagnostic tool for the initial assessment of HAIs, enhancing early detection and improving patient management across healthcare settings.
dc.identifier.citationKarakullukçu, A., Akker, M., Kuşkucu, M. A., Aygün, G., & Dikmen, Y. (2026). Evaluating a pathogen-specific IgG binding assay for rapid detection of healthcare-associated infections. Scientific Reports, 16, pp. 1-9. https://doi.org/10.1038/s41598-025-30459-7
dc.identifier.doi10.1038/s41598-025-30459-7
dc.identifier.endpage9
dc.identifier.issn2045-2322
dc.identifier.pmidPMID: 41723194
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1038/s41598-025-30459-7
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1381
dc.identifier.volume16
dc.identifier.wosWOS:001721730700002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorKarakullukçu, Asiye
dc.institutionauthorid0000-0002-7117-5102
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHealthcare-Associated Infections
dc.subjectPathogen-Specific Igg
dc.subjectIntensive Care Unit
dc.subjectRapid Diagnostic Test
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectAntibiotic Stewardship
dc.titleEvaluating a pathogen-specific IgG binding assay for rapid detection of healthcare-associated infections
dc.typeArticle
dspace.entity.typePublication

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