Synergistic effects of ozone with doxorubicin on the proliferation, apoptosis and metastatic profile of luminal-B type human breast cancer cell line

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dc.authorid0000-0002-6424-7411en_US
dc.authorscopusid57366670900en_US
dc.authorwosidCJT-9277-2022en_US
dc.contributor.authorKaragülle, Onur Olgaç
dc.contributor.authorGök Yurttaş, Asiye
dc.date.accessioned2023-11-14T07:33:30Z
dc.date.available2023-11-14T07:33:30Z
dc.date.issued2023en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalıen_US
dc.description.abstractObjective Luminal-B type human breast cancer cell line (BT-474) to assess the synergistic effects of ozone applied after chemotherapeutic treatment with various dosages of doxorubicin, and compare the results with the effects on L929 fibroblast cell line. Methods Doxorubicin (1–50 M) was added to each cell lines and left to sit for 24 h at 37 °C. Then, as combination groups, half of the groups were incubated with 30 g/mL ozone for 25 min. Tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and matrix metalloproteinase-2 and − 9 (MMP-2 and MMP-9) levels were measured using the MTT test, flow cytometry, and immunocytochemistry, respectively. Results When compared to simply doxorubicin-applied cells without ozone treatment, each dose of doxorubicin + ozone treatment considerably boosted L929 viability but significantly decreased BT-474 viability. Additionally, the combination increased the apoptotic impact of doxorubicin on BT-474 but not L929 (P < 0.001). TGF-, MMP-2, and MMP-9 levels of L929 after combination were substantially higher than those of the other groups (P < 0.01). Doxorubicin's effect on BT-474's protein levels, which had significantly decreased in comparison to those of the other groups, was reversed by the combination treatment (P < 0.05). Conclusion Doxorubicin's anti-proliferative and apoptotic effects were enhanced by ozone treatment in BT-474 cells, but it also repaired and healed healthy fibroblast cells that had been harmed by the cytotoxicity of the chemotherapy drug. If doxorubicin and ozone treatment are coupled, BT-474 cells may develop resistance to it through expressions of TNF-α, TGF-β, MMP-2, and MMP-9.en_US
dc.identifier.citationKaragülle, O. O., & Gök Yurttaş, A. (2023). Synergistic effects of ozone with doxorubicin on the proliferation, apoptosis and metastatic profile of luminal-B type human breast cancer cell line. Tissue and Cell, 85, pp. 1-7. https://doi.org/10.1016/j.tice.2023.102233en_US
dc.identifier.doi10.1016/j.tice.2023.102233en_US
dc.identifier.endpage7en_US
dc.identifier.issn1532-3072
dc.identifier.issn0040-8166
dc.identifier.pmidPMID: 37866151en_US
dc.identifier.scopus2-s2.0-85175201913en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.tice.2023.102233
dc.identifier.urihttps://hdl.handle.net/20.500.13055/582
dc.identifier.volume85en_US
dc.identifier.wosWOS:001098436400038en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorGök Yurttaş, Asiye
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTissue and Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast Canceren_US
dc.subjectDoxorubicinen_US
dc.subjectBT-474 Cellen_US
dc.subjectOzoneen_US
dc.subjectL929 Cellen_US
dc.titleSynergistic effects of ozone with doxorubicin on the proliferation, apoptosis and metastatic profile of luminal-B type human breast cancer cell lineen_US
dc.typeArticleen_US
dspace.entity.typePublication

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