Determination of carnosic acid by a novel HPLC-UV method in human plasma and application to a prototype pharmacokinetic study
Yükleniyor...
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Oxford Academic
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An HPLC method with UV detection was developed for the determination of carnosic acid in human plasma and applied to a pharmacokinetic study after oral administration of Rosemary extract to a healthy volunteer. Sample preparation depends on liquid-liquid extraction with hexane. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25°C with isocratic elution, mobile phase composed of solution A (methanol), and solution B (2% o-phosphoric acid in water) (90:10, v/v) at flow rate of 1.0 mL/min. The analyte was detected at 230 nm. The retention time is 4.20 ± 0.03 min. The method was validated in terms of accuracy, precision, specificity, robustness and detection and quantification limits, in accordance with European Medicines Agency guidelines. LOD and LOQ were found to be 0.075 and 0.25 ng/mL, respectively. The method was applied to the analysis of carnosic acid in human plasma with good recovery as 91.7%. The plasma concentration-time profile and pharmacokinetic parameters: AUC0-t, AUC0-∞, Cmax, tmax, t1/2 were calculated according to the assays. The method can certainly be used for routine analysis of carnosic acid in human plasma after oral administration of Rosemary extract, and for phase I clinical studies and bioavailability-bioequivalance studies as well.
Açıklama
Anahtar Kelimeler
UV Detection, Carnosic Acid, Diterpenes, Pharmacokinetics, Validation
Kaynak
Journal of Chromatographic Science
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
62
Sayı
1
Künye
Ceylan, B., Tırıs, G., & Kepekçi Tekkeli, Ş. E. (2024). Determination of carnosic acid by a novel HPLC-UV method in human plasma and application to a prototype pharmacokinetic study. Journal of Chromatographic Science, 62(1), pp. 21-26. https://doi.org/10.1093/chromsci/bmad005
