Determination of carnosic acid by a novel HPLC-UV method in human plasma and application to a prototype pharmacokinetic study

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dc.authorid0000-0002-1871-017Xen_US
dc.authorscopusid49964600400en_US
dc.authorwosidA-3672-2016en_US
dc.contributor.authorCeylan, Burhan
dc.contributor.authorTırıs, Gizem
dc.contributor.authorKepekçi Tekkeli, Şerife Evrim
dc.date.accessioned2023-02-27T06:26:20Z
dc.date.available2023-02-27T06:26:20Z
dc.date.issued2024en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalıen_US
dc.description.abstractAn HPLC method with UV detection was developed for the determination of carnosic acid in human plasma and applied to a pharmacokinetic study after oral administration of Rosemary extract to a healthy volunteer. Sample preparation depends on liquid-liquid extraction with hexane. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25°C with isocratic elution, mobile phase composed of solution A (methanol), and solution B (2% o-phosphoric acid in water) (90:10, v/v) at flow rate of 1.0 mL/min. The analyte was detected at 230 nm. The retention time is 4.20 ± 0.03 min. The method was validated in terms of accuracy, precision, specificity, robustness and detection and quantification limits, in accordance with European Medicines Agency guidelines. LOD and LOQ were found to be 0.075 and 0.25 ng/mL, respectively. The method was applied to the analysis of carnosic acid in human plasma with good recovery as 91.7%. The plasma concentration-time profile and pharmacokinetic parameters: AUC0-t, AUC0-∞, Cmax, tmax, t1/2 were calculated according to the assays. The method can certainly be used for routine analysis of carnosic acid in human plasma after oral administration of Rosemary extract, and for phase I clinical studies and bioavailability-bioequivalance studies as well.en_US
dc.description.sponsorshipBezmialem Vakıf University -- 20200203en_US
dc.identifier.citationCeylan, B., Tırıs, G., & Kepekçi Tekkeli, Ş. E. (2024). Determination of carnosic acid by a novel HPLC-UV method in human plasma and application to a prototype pharmacokinetic study. Journal of Chromatographic Science, 62(1), pp. 21-26. https://doi.org/10.1093/chromsci/bmad005en_US
dc.identifier.doi10.1093/chromsci/bmad005en_US
dc.identifier.endpage26en_US
dc.identifier.issn0021-9665
dc.identifier.issn1945-239X
dc.identifier.issue1en_US
dc.identifier.pmidPMID: 36828782en_US
dc.identifier.scopus2-s2.0-85181587022en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.1093/chromsci/bmad005
dc.identifier.urihttps://hdl.handle.net/20.500.13055/400
dc.identifier.volume62en_US
dc.identifier.wosWOS:000945061000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorKepekçi Tekkeli, Şerife Evrim
dc.language.isoenen_US
dc.publisherOxford Academicen_US
dc.relation.ispartofJournal of Chromatographic Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUV Detectionen_US
dc.subjectCarnosic Aciden_US
dc.subjectDiterpenesen_US
dc.subjectPharmacokineticsen_US
dc.subjectValidationen_US
dc.titleDetermination of carnosic acid by a novel HPLC-UV method in human plasma and application to a prototype pharmacokinetic studyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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