Determination of the toxicity preferences of ocular drug delivery system by comparing two different toxicity bioassays
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mary Ann Liebert
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ocular drug delivery methods are highly favored for boosting bioavailability, patient compliance, and lower adverse effects and dose frequency. In addition to preventing adverse effects from the active ingredient, the parts of drug delivery systems must be nontoxic and nonallergic as well. Mitochondrial toxicity test (MTT) and Hen's egg chorioallantois membrane (HET-CAM) assay are the most often utilized tests based on this dilemma. The toxicity of loteprednol etabonate loaded solid lipid nanoparticles, lipid nanostructured carriers, and nanoemulsion were compared. Oleic acid, Precirol®ATO5, and Pluronic® F68 were used in the preparation. Their toxicities were evaluated by using two different toxicity tests (MTT and HET-CAM). The results suggest that there are no significant differences between the HET-CAM and MTT assays. It is noteworthy that the HET-CAM assay offers a more cost-effective and environmentally friendly alternative to the MTT assay, as it does not require cell culture and generates less toxic waste. This information may be useful to consider when selecting between the two assays.
Açıklama
Anahtar Kelimeler
HET-CAM, MTT, Nanoparticular Delivery, Ocular Targeting
Kaynak
ASSAY and Drug Development Technologies
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
21
Sayı
7
Künye
Üner, B., Durgun, M. E., Özdemir, S., Taş, Ç., Üner, M., & Özsoy, Y. (2023). Determination of the toxicity preferences of ocular drug delivery system by comparing two different toxicity bioassays. Assay and Drug Development Technologies, 21(7), pp. 337-343. https://doi.org/10.1089/adt.2023.058
