Effect of Aza-BODIPY-photodynamic therapy on the expression of carcinoma-associated genes and cell death mode

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dc.authorid0000-0002-6424-7411en_US
dc.authorscopusid57366670900en_US
dc.authorwosidCJT-9277-2022en_US
dc.contributor.authorElgün, Tuğba
dc.contributor.authorGök Yurttaş, Asiye
dc.contributor.authorÇınar, Kamil
dc.contributor.authorÖzçelik, Şennur
dc.contributor.authorGül, Ahmet
dc.date.accessioned2023-10-26T12:33:51Z
dc.date.available2023-10-26T12:33:51Z
dc.date.issued2023en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalıen_US
dc.description.abstractBackground: Breast cancer is the most common cancer affecting women worldwide.Photodynamic therapy(PDT) has now proven to be a promising form of cancer therapy due to its targeted and low cytotoxicity to healthy cells and tissues.PDT is a technique used to create cell death localized by light after application of a light-sensitive agent.Aza-BODIPY is a promising photosensitizer for use in PDT. Our results showed that aza-BODIPY-PDT induced apoptosis, probably through p53 and caspase3 in MCF-7 cells. Future studies should delineate the molecular mechanisms underlying aza-BODIPY-PDT-induced cell death for a better understanding of the signaling pathways modulated by the therapy so that this novel technology could be implemented in the clinic for treating breast cancer. Aim: In this study,we aimed to determine the change in the expression levels of 88 carcinoma-associated genes induced by aza-BODIPY-PDT were analyzed so as to understand the specific pathways that are modulated by aza-BODIPY-PDT. Material method: In this study,the molecular basis of the anti-cancer activity of aza-BODIPY-PDT was investigated.Induction of apoptosis and necrosis in MCF-7 breast cancer cells after treatment with aza- BODIPY derivative with phthalonitrile substituents (aza-BODIPY) followed by light exposure was evaluated by Annexin V 7- Aminoactinomycin D (7-AAD) flow cytometry. Results: Aza-BODIPY-PDT induced cell death in MCF-7 cells treated with aza-BODIPY-PDT; flow cytometry revealed that 28% of the cells died by apoptosis. Seven of the 88 carcinoma-associated genes that were assayed were differentially expressed -EGF, LEF1, WNT1, TCF7, and TGFBR2 were downregulated, and CASP3 and TP53 were upregulated - in cells subjected to aza-BODIPY-PDT.This made us think that the aza-BODIPY-PDT induced caspase 3 and p53-mediated apoptosis in MCF7 cells. Conclusion: In our study,it was determined that the application of aza-BODIPY-PDT to MCF7 cells had a negative effect on cell connectivity and cell cycle.The fact that the same effect was not observed in control cells and MCF7 cells in the dark field of aza-BODIPY indicates that aza-BODIPY has a strong phodynamic anticancer effect.en_US
dc.identifier.citationElgün, T., Gök Yurttaş, A., Çınar, K., Özçelik, Ş., & Gül, A. (2023). Effect of Aza-BODIPY-photodynamic therapy on the expression of carcinoma-associated genes and cell death mode. Photodiagnosis and Photodynamic Therapy, 44, pp. 1-12. https://doi.org/10.1016/j.pdpdt.2023.103849en_US
dc.identifier.doi10.1016/j.pdpdt.2023.103849en_US
dc.identifier.endpage12en_US
dc.identifier.issn1572-1000
dc.identifier.pmidPMID: 37863378en_US
dc.identifier.scopus2-s2.0-85176235733en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.pdpdt.2023.103849
dc.identifier.urihttps://hdl.handle.net/20.500.13055/565
dc.identifier.volume44en_US
dc.identifier.wosWOS:001111789700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorGök Yurttaş, Asiye
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhotodynamic Therapyen_US
dc.subjectMCF7 Cancer Cellen_US
dc.subjectAza-BODIPY-PDTen_US
dc.subjectGene Expressionen_US
dc.titleEffect of Aza-BODIPY-photodynamic therapy on the expression of carcinoma-associated genes and cell death modeen_US
dc.typeArticleen_US
dspace.entity.typePublication

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